cdna preparation and characterization

... Northern blots containing a variety of candidate cDNAs, or cDNA arrays containing from just a few to several thousand candidate cDNAs. 3°31 cDNA Synthesis and Antisense RNA Amplification of mRNA ... treatment, phenol-chloroform and chloroform extraction, and ethanol precipitation following standard procedures; first- and second-strand cDNA primers (see text for sequence and...

Ngày tải lên: 10/04/2014, 11:12

... doped polybenzimidazole and its composites with inorganic proton conductors. J. Membr. Sci, 2003, 226(1-2):169-184 [15] C. W. Lin, K. C. Fan, R. Thangmuthu. Preparation and characterization of ... polymerization and phase inversion, and all of the other reagents were analytical grade. 2.2 Preparation of the PVDF-based composite membrane The PVDF powders were basified fir...

Ngày tải lên: 05/09/2013, 16:11

... VNU Journal of Science, Mathematics - Physics 24 (2008) 42-46 42 Preparation and characterization of nanocomposite TiO 2 /SnO 2 films Pham Van Nho 1,* , Tran Kim Cuong 2 ... Abstract: This work presents the preparation of nanocomposite TiO 2 /SnO 2 films by using spray pyrolysis and followed by sol-gel technique from TiCl 4 and SnCl 4 solutions. Obtained films ... a comb...

Ngày tải lên: 13/02/2014, 04:20

... the first, second and third EF-hand domains in oyster CaM and CaLP, while it reflected Ca 2+ -binding to the first and second EF-hand domains in the case of VanScyoc et al. CaLP and CaM chromatography ... in the sec- ond and the fourth EF-hand domains are more con- served than those in the first and the third EF-hand domains compared with oyster CaM. Structural varia- tions in EF-hand...

Ngày tải lên: 16/03/2014, 23:20

... Preparation and characterization of CNTs–TiO 2 composites Li Chen * , Bai-Lan Zhang, Mei-Zhen Qu, Zuo-Long ... such as C–O, C=O and O – C=O which might stimulate formation of the composites, and enhance the interfacial combination of TiO 2 with carbon nanotubes. The formation of TiO 2 and its compounding ... ethanol for 2 min and then a drop of dispersion was deposited on a Cu/R...

Ngày tải lên: 20/03/2014, 13:06

... Introduction The synthesis and characterization of nanostructured materi- als (nanotubes, nanorods, nanowires, and nanosheet) have received considerable attention due to their unique properties and novel applications ... samples were characterized by XRD, SEM, TEM, SAED, and BET surface area. The nanosheet structure was slightly curved and approximately 50–100 nm in width and se...

Ngày tải lên: 20/03/2014, 13:06

... Dohna ´ lek, in preparation. [34] O. Bondarchuk, J. Kim, J.M. White, B.D. Kay, Z. Dohna ´ lek, in preparation. J. Kim et al. / Catalysis Today 120 (2007) 186–195 195 Preparation and characterization ... Cyclic trimers 1. Introduction The preparation and characterization of nanoclusters on supporting surfaces remain significant challenges for nanoscience in general and especia...

Ngày tải lên: 20/03/2014, 13:06

... and moulds, and then very early recruited in the metazo- ans. Here we report the expression and purification of recombinant geodin and its thermodynamic and spect- roscopic characterization, and ... investigated: the soluble fraction (S preparation) and the pelleted fraction, after solubilization and renaturation (see Methods), called IB preparation. The S and IB preparati...

Ngày tải lên: 30/03/2014, 15:20

... 5¢-or 3¢-RACE reaction, the cDNA was amplified by RT-PCR with the latex poly(A) + RNA as the template and then sequenced. Finally, two cDNAs were obtained and desig- nated HRT1 and HRT2, respectively. DNA ... containing YPD medium and incubated at 23 °Cor37°C. Results Isolation and characterization of HRT cDNAs A pair of degenerate primers designed from two highly conserved regio...

Ngày tải lên: 07/03/2014, 21:20

... inhibitor. Results Cloning and sequence analysis of full-length tryptase cDNAs A partial cDNA (690 bp) encoding a new bovine tryptase isoform (BLT) was obtained from lung mRNA by RT-PCR, using primers N9 and N10, and ... (1989) Cloning and characterization of complementary DNA for human tryptase. J. Clin. Invest. 84, 1188–1195. 24. Miller, J.S., Moxley, G. & Schwartz, L.B. (19...

Ngày tải lên: 17/03/2014, 09:20