basic dna and rna protocols
basic dna and rna protocols
... of enzyme required to digest 1 p.g
of a standard DNA in 1 h under optimal temperature and buffer conditions.
The standard DNA used is normally h DNA. Hence, for EcoRI (for
example), there ... bacteriophages and other viruses by agarose gel
electrophoresis. J Vzrol 14, 1235-1244.
CHAPTER
1
The Simultaneous Isolation
of RNA and DNA
from Tissues and Cultured Cells
Fran...
Tài liệu Báo cáo khoa học: Identification of Ewing’s sarcoma protein as a G-quadruplex DNA- and RNA-binding protein ppt
... ETS-1
(lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per-
formed with EWS (lanes 2, 4 and 6) and
32
P-labeled Htelo (lanes 3
and 4), dsHtelo (lanes 5 and 6) or ssDNA S (lanes 1 and 2). The
structures ... shift assay (EMSA)
with EWS and several G-quadruplex or single- or dou-
ble-stranded DNA and RNA. Here, we show that
EWS specifically targets G-quadruplex DNA...
Tài liệu Báo cáo Y học: DNA and RNA damage by Cu(II)-amikacin complex docx
... yeast tRNA
Phe
, induced by Ami and its
Cu(II) complex. 5¢-end-labeled tRNA
Phe
was mixed with unlabeled
tRNA
Phe
to obtain final concentration of 1 l
M
. Lane 1, untreated
tRNA
Phe
,lane2,tRNA
Phe
+50l
M
Cu(II) ... Biotech, Wrocław,
Poland).
Isolation of yeast tRNA
Phe
Yeast tRNA
Phe
of specific phenylalanine acceptance
1200–1400 pmol per A
260
unit was prepared from crude
baker’s yeast tRNA...
Tài liệu Báo cáo Y học: Temperature dependence of thermodynamic properties for DNA/DNA and RNA/DNA duplex formation pdf
... properties
for DNA/ DNA and RNA /DNA duplex formation
Peng Wu
1,
*, Shu-ichi Nakano
1
and Naoki Sugimoto
1,2
1
High Technology Research Center and
2
Department of Chemistry, Faculty of Science and Engineering, ... para-
meters of the 24 DNA/ DNA and 41 RNA /DNA duplexes
at standard temperature (25 °C) and physiological
temperature (37 °C). Direct comparison shows that the...
Báo cáo khoa học: Human telomeric G-quadruplex: structures of DNA and RNA sequences ppt
... human telomeres. These include: (a)
the structure and dynamics of all possible DNA, RNA
and DNA ⁄ RNA hybrid G-quadruplexes formed by
short and long human telomeric sequences; (b) the
structural ... one strand and only
the 3¢-end G-tract of the other strand [30] (Fig. 3D).
The core of this structure, called the (3 + 1) core, has
three strands oriented in one direction and one...
basic protein and peptide protocols
... for two alternative protein standards, bovine serum albumin and
ovalbumin, are 0.66 and 0.75, respectively.
3. Plastic and glassware used in the assay should be absolutely clean and
detergent-free. ...
band of about 1 cm width.
4. Notes
1. The reducing agent in the sample solvent reduces mtermolecular disul-
fide bridges and so destroys quarternary structure and separates s...
rna isolation and characterization protocols
... protein and DNA partition into
the acid phenol phase leaving the RNA in the aqueous phase. The method de-
scribed below uses SDS to lyse cells and acidic phenol to remove DNA and
proteins, and ... RNA 1s separated from cellular DNA and
proteins by centrifugation through 5.7MCsC1, or by acid phenol extraction. In
the former, the RNA passes through the 5.7MCsCl but pr...
Báo cáo y học: "Parvovirus B19 Nonstructural Protein-Induced Damage of Cellular DNA and Resultant Apoptosis"
... attached to DNA in a covalent manner,
and that both DNA- helix distorting and single strand
nick forms of DNA damage are important pathways
to apoptosis upon expression of NS1.
Materials and Methods ... from chromosomal DNA damage caused by the
DNA- nicking and DNA- attaching activities of NS1. Upon testing this hypothesis, we found
that NS1 covalently binds to cellular...
Báo cáo y học: "HIV DNA and Dementia in Treatment-Naïve HIV-1-Infected Individuals in Bangkok, Thailand"
... in Bangkok, Thailand. Subjects with and without HAD were recruited and matched for age,
gender, education, and CD4 cell count. PBMC and cellular subsets were analyzed for HIV DNA using real-time ... clades A, E,
and B; and demonstrated equivalent amplification of
the target gene.
Separation of PBMC Subsets and HIV DNA Analay-
sis.
Because HIV DNA is present in both lym...
Introduction to the basic approaches and issues of Intrusion Detection
... and personnel) in standby mode
* Review and update INFOCON ORANGE and INFOCON RED contingency plans
Owners and Operators:
* Round-the-clock staffing of Sector Center
* Report new Category I and ... (software and personnel) in standby mode
* Review and update INFOCON ORANGE and INFOCON RED contingency plans
Owners and Operators:
* Report new Category I, II, and III incident...