basic dna and rna protocols

... of enzyme required to digest 1 p.g of a standard DNA in 1 h under optimal temperature and buffer conditions. The standard DNA used is normally h DNA. Hence, for EcoRI (for example), there ... bacteriophages and other viruses by agarose gel electrophoresis. J Vzrol 14, 1235-1244. CHAPTER 1 The Simultaneous Isolation of RNA and DNA from Tissues and Cultured Cells Fran...

Ngày tải lên: 10/04/2014, 11:03

... ETS-1 (lanes 3 and 4) or ssDNA L (lanes 1 and 2). (B) EMSA was per- formed with EWS (lanes 2, 4 and 6) and 32 P-labeled Htelo (lanes 3 and 4), dsHtelo (lanes 5 and 6) or ssDNA S (lanes 1 and 2). The structures ... shift assay (EMSA) with EWS and several G-quadruplex or single- or dou- ble-stranded DNA and RNA. Here, we show that EWS specifically targets G-quadruplex DNA...

Ngày tải lên: 15/02/2014, 01:20

... yeast tRNA Phe , induced by Ami and its Cu(II) complex. 5¢-end-labeled tRNA Phe was mixed with unlabeled tRNA Phe to obtain final concentration of 1 l M . Lane 1, untreated tRNA Phe ,lane2,tRNA Phe +50l M Cu(II) ... Biotech, Wrocław, Poland). Isolation of yeast tRNA Phe Yeast tRNA Phe of specific phenylalanine acceptance 1200–1400 pmol per A 260 unit was prepared from crude baker’s yeast tRNA...

Ngày tải lên: 21/02/2014, 01:21

... properties for DNA/ DNA and RNA /DNA duplex formation Peng Wu 1, *, Shu-ichi Nakano 1 and Naoki Sugimoto 1,2 1 High Technology Research Center and 2 Department of Chemistry, Faculty of Science and Engineering, ... para- meters of the 24 DNA/ DNA and 41 RNA /DNA duplexes at standard temperature (25 °C) and physiological temperature (37 °C). Direct comparison shows that the...

Ngày tải lên: 22/02/2014, 07:20

... human telomeres. These include: (a) the structure and dynamics of all possible DNA, RNA and DNA ⁄ RNA hybrid G-quadruplexes formed by short and long human telomeric sequences; (b) the structural ... one strand and only the 3¢-end G-tract of the other strand [30] (Fig. 3D). The core of this structure, called the (3 + 1) core, has three strands oriented in one direction and one...

Ngày tải lên: 06/03/2014, 09:22

... for two alternative protein standards, bovine serum albumin and ovalbumin, are 0.66 and 0.75, respectively. 3. Plastic and glassware used in the assay should be absolutely clean and detergent-free. ... band of about 1 cm width. 4. Notes 1. The reducing agent in the sample solvent reduces mtermolecular disul- fide bridges and so destroys quarternary structure and separates s...

Ngày tải lên: 10/04/2014, 11:03

... protein and DNA partition into the acid phenol phase leaving the RNA in the aqueous phase. The method de- scribed below uses SDS to lyse cells and acidic phenol to remove DNA and proteins, and ... RNA 1s separated from cellular DNA and proteins by centrifugation through 5.7MCsC1, or by acid phenol extraction. In the former, the RNA passes through the 5.7MCsCl but pr...

Ngày tải lên: 11/04/2014, 10:30

... attached to DNA in a covalent manner, and that both DNA- helix distorting and single strand nick forms of DNA damage are important pathways to apoptosis upon expression of NS1. Materials and Methods ... from chromosomal DNA damage caused by the DNA- nicking and DNA- attaching activities of NS1. Upon testing this hypothesis, we found that NS1 covalently binds to cellular...

Ngày tải lên: 25/10/2012, 11:18

... in Bangkok, Thailand. Subjects with and without HAD were recruited and matched for age, gender, education, and CD4 cell count. PBMC and cellular subsets were analyzed for HIV DNA using real-time ... clades A, E, and B; and demonstrated equivalent amplification of the target gene. Separation of PBMC Subsets and HIV DNA Analay- sis. Because HIV DNA is present in both lym...

Ngày tải lên: 31/10/2012, 15:34

... and personnel) in standby mode * Review and update INFOCON ORANGE and INFOCON RED contingency plans Owners and Operators: * Round-the-clock staffing of Sector Center * Report new Category I and ... (software and personnel) in standby mode * Review and update INFOCON ORANGE and INFOCON RED contingency plans Owners and Operators: * Report new Category I, II, and III incident...

Ngày tải lên: 04/11/2013, 13:15