molecular analysis of cancer - carrie fidler
molecular analysis of cancer - carrie fidler
... of Molecular Cancer Genetics 1
1
From:
Methods in Molecular Medicine, vol. 68: Molecular Analysis of Cancer
Edited by: J. Boultwood and C. Fidler © Humana Press Inc., Totowa, NJ
1
Molecular Analysis ... buffer: 0.5 M Tris-HCl, pH 7.5, 50 mM MgCl
2
, 0.5 mg/mL
of nuclease-free bovine serum albumin (BSA).
3. 1 mM Biotin-16-dUTP (bio-16-dUTP), 1 mM digoxigenin-11-dUTP (di...
Tài liệu MULTICOLOR QUANTUM DOTS FOR MOLECULAR DIAGNOSTICS OF CANCER doc
... judgment is com-
monly left to the expert opinion of a patho-
logist who studies the tumor biopsy. With the
advent of high-throughput data analysis of
genomic and proteomic classifications of
cancer ... into
one of various subtypes, each of which can have
drastically different prognoses and preferred
methods of treatment. Diagnosis of cancer sub-
types is vitally importa...
Báo cáo khoa học: Molecular analysis of the interaction between cardosin A and phospholipase Da Identification of RGD/KGE sequences as binding motifs for C2 domains pdf
... heterotrimeric G-protein a-sub-
unit through a motif analogous to the DRY motif in
G-protein-coupled receptors. J Biol Chem 279, 1794–
1800.
47 Cruz de Carvalho MH, d’Arcy-Lameta A, Roy-Macau-
ley H, ... the buffer used for S-300 chroma-
tography. Elution was carried out with a linear gradient of
NaCl (0–0.5 m) at a flow rate of 1.0 mLÆmin
)1
. The wild-
type and mutated forms of recombi...
A gene expression database for the molecular pharmacology of cancer pptx
... HL-60(TB) and K-562; non-small cell lung cancer NCI-
H226; colon cancer COLO 205; CNS cancer SNB-19; melanoma LOX-IMVI;
ovarian cancers OVCAR-3 and OVCAR–4; renal cancer CAKI-1; prostate can-
cer ... cells. MDA-N and MDA-MB-435 clustered with
three of the melanoma lines (M14, UACC-62 and SK-MEL-5).
Breast cancer lines HS 578T, MDA-MB-231 and BT-549 clus-
tered together, but far fr...
Tài liệu Báo cáo khoa học: EGF receptor in relation to tumor development: molecular basis of responsiveness of cancer cells to EGFR-targeting tyrosine kinase inhibitors docx
... of Akt- and ERK1 ⁄ 2-dependent pathways eventu-
ally change the expression level of one or more of
these Bcl-2 family members.
Bad
Bad is one of the ‘death-promoting’ members of the
Bcl-2 family, ... induction of Bim in
gefitinib-triggered apoptosis of NSCLC cells.
Bax
Bax is a 21-kDa multi-BH domain pro-apoptotic pro-
tein and acts downstream of BH3-only proteins. The
inductio...
Tài liệu Báo cáo khoa học: Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching doc
... primers
5¢-GGAGATCTAAAATGGACGACTGGGAGGAAGA-3¢
and 5¢-GCGAATTCGTTGAAAACTTTTAATTATCA
GGAGAAAAC-3¢,5¢-CGAGATCTAGCATGTCAGACG
TGGAGTCTGGA-3¢ and 5¢-GCGAATTCGCAACCAAA
GACAACCTGGTTTTAATGTTTTGA-3¢, and 5¢-CGAG
ATCTGAAATGAACGAGCTGCGTATGCCGAA-3¢ ... HeLa cells. Time-
lapse LSM image analysis of the Olvas–GFP distribu-
tion reveals that the shape of the nuage changes in a
matter of minutes in mi...
Tài liệu Báo cáo Y học: NMR-based determination of the binding epitope and conformational analysis of MUC-1 glycopeptides and peptides bound to the breast cancer-selective monoclonal antibody SM3 pptx
... 3.05
Arg4-a/Arg4-dd¢ 3.10 3.42
Arg4-a/Arg4-NH 2.57 2.84
Arg4-a/Pro5-dd¢ 2.30 2.55
Arg4-cc¢/Arg4-NH 2.97 3.28
Arg4-NH/Arg4-bb¢ 2.75 3.04
Thr3-c/Thr3-a 3.02 3.34
Thr3-a/Thr3-NH 3.26 3.60
Thr3-a/Arg4-NH ... (A
˚
)
Asp2-a/Thr3-NH* 2.35 2.60
GalNAc-H1/Thr3-NH* 2.35 2.60
Pro5-dd¢/Arg4-cc¢ 3.03 3.35
Arg4-a/Arg4-dd¢ 3.17 3.51
Arg4-a/Arg4-cc¢ 2.48 2.74
Arg4-a/Pro5-dd¢ 2.33 2.58
Arg4-bb¢/Arg4-dd¢ 2.99...
Báo cáo khoa học: Molecular cloning of the Matrix Gla Protein gene from Xenopus laevis Functional analysis of the promoter identifies a calcium sensitive region required for basal activity doc
... oligonucleotide (5¢-CACGC
AAGCTTCT
CTTGAGTCTCTATGAAGG-3¢)andthe5¢ specific oli-
gonucleotides (5¢-CCGGAGCTC
GAGACTCTTAGTAA
ATGTGCCCC-3¢) for amplification of the fragment from
)94 9 to + 33 (5 ¢-CCG
GAGCTCGAGCCGCTAAAGA
1948 ... particular, calcium-
containing-mineral such as hydroxyapatite [2].
Although the exact m ode of action of MGP at the
molecular level is currently unknown, the s...
Analysis of Assembly Bill 1774 Health Care Coverage: Gynecological Cancer Screening Tests potx
... types of
gynecological cancers: cervical cancer, ovarian cancer, and endometrial or uterine cancer. As
indicated in the Introduction, these cancers account for 90% of all gynecological cancers ... University of California’s Office of the President supports a task
force of faculty from several campuses of the University of California, as well as Loma Linda
University, the...
Impact of Molecular Biology on Cancer Treatment: I Therapeutic Targets potx
... several cancers their activity is up-regulated by
gain -of- function mutations or over- e x p ression. PTK
activity can be up-regulated by several mechanisms:
genomic re-arrangements e.g. B C R - A ... selectively suppresses the
proliferation of BCR-ABL-positive cells and is an
example of a rationally designed, molecular-
targeted drug for the treatment of a specific cancer
( C...
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