... Chemistry, Vol. 42 ( 1), P. 115 - 120, 2004 isomeranzin against Herpes simplex virus in vitro from clausena heptaphylla (Roxb. ) W. & Arn. : isolation, structure and biological assay Received ... coumarin was isolated from the leaves of Clausena heptaphylla. The structure was elucidated by IR, MS, 1 H and 1 3 C-NMR. For the first ti...
Ngày tải lên: 03/04/2014, 15:20
... TS, Lindsay KL, Levine AM & Lai MM (200 3) Establishment of B-cell lymphoma cell lines persistently infected with hepatitis C virus in vivo and in vitro: the apoptotic effects of virus infection. ... Notvall L & Bigger C (200 3) Antiviral effect and virus host interactions in response to alpha interferon, gamma interferon, poly(I)–poly(C), tumor necrosis factor a...
Ngày tải lên: 16/03/2014, 05:20
Báo cáo khoa học: 7,8-Diaminoperlargonic acid aminotransferase from Mycobacterium tuberculosis, a potential therapeutic target Characterization and inhibition studies pptx
... 1), were shown to be suicide sub- strates of this enzyme, with the following inactivation parameters: K i ¼ 12±2lm, k inact ¼ 0.35 ± 0.05 min )1 , and K i ¼ 20±2lm, k inact ¼ 0.56 ± 0.05 min )1 , ... apply, and the following kinetic parameters, K i and k inact , were derived from a Kitz–Wilson plot (Fig. 6B), for amiclenomycin and compound 1, respectively: K i ¼ 12±2lm, k...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: B1–Proteases as Molecular Targets of Drug Development B1-001 DPP-IV structure and inhibitor design ppt
... mediated by WW-domain 3. SGK also has a PY-motif, therefore we tested whether the WW domains of Nedd4 and Nedd4-2 mediate bind- ing to SGK. We show that single or tandem WW domains of Nedd4 and Nedd4-2 ... Oceanography (PIN- RO), Murmansk, Russian Federation. E-mail: vmukhin@pinro.ru Dry hydrolysate was prepared from protein-containing waste of Icelandic scallop Chlamys islandicus proce...
Ngày tải lên: 23/03/2014, 15:20
Tài liệu Báo cáo khoa học: Role for nectin-1 in herpes simplex virus 1 entry and spread in human retinal pigment epithelial cells docx
... changes in the cytoskeleton that may occur during the initial 6 h window of infection. We infected RPE cells with K26GFP [27], and stained cells for F-actin, using phalloidin at 30 min and 6 h postinfection, ... with red plasmid (DS-Red; BD Biosciences). At 18 h postmixing, the cells were fixed and permeabilized, and this was followed by F-actin staining with 10 nm rhodamine-conjugat...
Ngày tải lên: 18/02/2014, 14:20
Báo cáo khoa học: Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry potx
... double- stranded DNA viruses divided into a, b and c subgroups [1]. Only eight herpesviruses are known to commonly infect humans and the remainder are animal herpesviruses infecting a wide variety ... viral entry. J Clin Invest 108, 503–510. 2 Whitley RJ & Roizman B (200 1) Herpes simplex virus infections. Lancet 357, 1513–1518. 3 Spear PG (200 4) Herpes simplex virus...
Ngày tải lên: 23/03/2014, 04:20
Báo cáo khoa học: Adipophilin protein expression in muscle – a possible protective role against insulin resistance pot
... Flier JS (200 0) Obesity and insulin resistance. J Clin Invest 106, 473–481. 6 Taniguchi CM, Emanuelli B & Kahn CR (200 6) Critical nodes in signalling pathways: insights into insulin action. ... Adfp in muscle cells, we cultured C2C12 cells in differentiation medium containing one of the following agonists: GW7647 (PPARa), WY14643 (PPARa), GW501516 (PPARb ⁄ d) and rosigli...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: A structured RNA in hepatitis B virus post-transcriptional regulatory element represses alternative splicing in a sequence-independent and position-dependent manner pot
... exclusively, most splicing enhancers tend to bind SR proteins, a family of proteins containing one or two RNA-binding domains and a signature RS domain rich in Arg ⁄ Ser dipeptides, and splicing silencers ... contains two stems (S1 and S 2) and a hexanucleotide loop (L 1), whereas HP2 contains a 9-bp stem (S 3) and a 25-nucle- otide large loop (L 2). HP1 and HP2 are j...
Ngày tải lên: 28/03/2014, 23:20
Báo cáo khoa học: Prohibitin is expressed in pancreatic b-cells and protects against oxidative and proapoptotic effects of ethanol pdf
... experi- ments). (A) Hoechst 33342 (nuclei) staining; (B) Mitofluor Red 589 (mitochondria) stain- ing; (C) anti-His (exogenous PHB)-FITC stain- ing; (D) merge. The arrows indicate staining of His-tagged ... ethanol and PHB in RINm5F cells. RINm5F cells were incubated for 24 h with or without ethanol (E, 80 m M) and with or without PHB (P, 10 n M) in the presence of glucose (G 1: 5.5...
Ngày tải lên: 29/03/2014, 08:20
Báo cáo khoa học: Adeno-associated virus gene transfer in Morquio A disease – effect of promoters and sulfatase-modifying factor 1 pot
... particular cases, including our study, remains unknown. However, in vitro [60] and in vivo [61] studies have shown that AAV vectors induce a change in gene expression profile. Genes involving cellular ... fetal bovine serum 15%, penicillin 100 UÆmL )1 and streptomycin 100 UÆmL )1 ] was removed immediately before starting the transfection. Plasmids were mixed in 18 : 18...
Ngày tải lên: 29/03/2014, 21:20