structural levels of the iliad

... levels to the war. These structural levels are of special interest, because they help define the consequences and outcomes of the war. The universal war of the gods, social war of the Greeks ... Trojans, and the private war of Achilles' honor are structural levels of the Trojan war. These structural levels seem to influence and shape each other...

Ngày tải lên: 02/04/2014, 17:58

... b ath for the required amount of time. At the end of the heat treatment the mixtures were placed in an ice/water bath, and the enzymatic activity w as stopped by lowering the pH of the r eaction ... BLG/protease mixture was then placed in a water bath thermostatted at 60 °C for the given amount of time . At the end of the heat treatment the mixture s were place...

Ngày tải lên: 21/02/2014, 15:20

... from the relative rates of fragment formation, the cleavages of the sites were in the order: Tyr146/Asn147 ! Phe114 ! other sites. The weakness of bands of fragments I and IV and the appearance of ... other; and (c) the hydrolysis of the Phe114-Ser115 bond was the rate determining step in autolytic inactivation. Thus, it is the cleavage of the interdomain l...

Ngày tải lên: 22/02/2014, 07:20

... were inserted between the third letter of one codon and the first letter of the following codon. Molecular modeling of the C. sepium b-amylase The amino-acid sequence of the b-amylase from C. ... built for the b-amylase from C. sepium using the X-ray coordinates of the soybean b-amylase (Fig. 4). According to the Ramachandran plot of this model the f and c ang...

Ngày tải lên: 22/02/2014, 07:20

... confirmed by the kinetic analysis of a purified GPI-anchorless soluble version of the mutant protein. The K cat ⁄ K m value of the mutant TNSALP was less than one-tenth of the K cat ⁄ K m value of the ... TNSALP (R433C). The rate of the intracellular transport of the mutant protein was similar to that of the wild-type protein, as assessed by the acquisition...

Ngày tải lên: 07/03/2014, 05:20

... than the value of 0.79 expected from theoretical consider- ations at a field strength of 11.7 T [37]. These results (together with those of the R 2 described above) suggest a high flexibility of the ... [31]. The residues with low NOE values (< 0.65) in CACW40A were Ile9 (at the C-cap of the 3 10 -helix); Tyr20 (at the beginning of the first helix); Lys26 and Ala30...

Ngày tải lên: 07/03/2014, 06:20

... of Cyn d 24. (A) Nucleotide and deduced amino acid sequences of Cyn d 24. The numbers on the right of the figure indicate the positions of the nucleotide sequence. The numbers on the left of the ... [32,33]. Formation of this type of oligosaccharide in BG60 has been suggested to be the result of degradative reac- tions, rather than imperfect biosynthesis [13]....

Ngày tải lên: 07/03/2014, 12:20

... identified by the mass increase of 42 mass units. The relative level of acetylation of a peptide was estimated on the basis of the intensity ratio of the native and acetylated species. From the data ... a-helix in the whole protein, and the 1–70 region is poorly structured. SDS increases the a-helix content of proteins revealing the helical potential. The a-he...

Ngày tải lên: 07/03/2014, 14:20

... the theoretical mass of the human PKIb (8468.2 Da) [11]. The identification of the gene product of human PKIb excluded it from the interference of human PKIb-70, which was translated from another ... m M sodium acetate buffer. The pooled fraction was stored at )20 °C for further study. The concentration of the proteins was determined by the method of Lowry et al....

Ngày tải lên: 07/03/2014, 15:20

... thought they were able to bind the ST peptide (Fig. 2). Therefore, there appeared to be a correlation between the presence of the 145 kDa form of GC-C, which represents the mature glycosylated form of ... representing the amount of GC-C detected by ST binding (Fig. 2C). Therefore, the fraction of GC-C present in the membranes of desensitized cells possessed guanylyl...

Ngày tải lên: 08/03/2014, 08:20