... tissues contain twoEcR isoforms, EcR-A and EcR-B1, EcR-A isoform ispredominantly expressed in the ASGs at pupation inBombyx [26], and ther efore we used anti-EcR-A s erum for the Western blotting. ... expressionrequired for completion of PCD is accomplished during t hefirst 8 h of 20E challenge, but withdrawal of 20E before30 h of the culture interferes with t he PCD sequence [14]. If the genomic theory of ... transcriptionalactivity of target genes [ 6]. I n addition, a nongenomic action of 20E has been supposed for decades. 20E increases the cellular c AMP level in the prothoracic glands of Manducasexta [7] and in the...