Báo cáo Y học: NMR structure of the HIV-1 regulatory protein Vpr in H2O/trifluoroethanol Comparison with the Vpr N-terminal (1–51) and C-terminal (52–96) domains pot
... modifications
when comparing the isolated domains of (1–51 )Vpr [25] and
(52–96 )Vpr [26] with the intact Vpr protein. The differences
in structure are localized to the N-terminal domain. The
secondary structures ... portion of
the protein and allow its tight binding to Lys-tRNA
synthetase [15].
The C-terminal domain (52–96 )Vpr and not the
N-term...
... pointing in opposite directions. The stabilization of
the folded conformation can be explained by the stacking of
the Val3 side chain with the Pro7 ring and by a hydrophobic
cluster formed by the ... purifying and handling integral
membrane proteins. The instability of these proteins in
environments lacking phospholipids and the tendency for
them to aggregate...
... by modification of the standard
cysteine residue in the
DYANA library [53]. A pseudoatom
was included instead of the HG atom and a bridge was
formed by superimposition of the pseudoatoms on the ... reduced mobility with respect to
the rest of the structure. Further studies of the receptor
bound forms of the chimera and other related ligands will be
necess...
... surprising similarity of this structure, as
well as the sequence of the C-terminal moiety, with those
of the fusion domain of in uenza hemagglutinin suggests a
direct mechanism of neurotoxicity.
Keywords: ... been
proposed for this structure [37].
TheoverallshapeofAb-(1–42) is strongly reminiscent of
the structure of the fusion domain of in uenza hemagglu-...
... remaining domains of gp120 in the
native structure. The carboxy terminal end of the C5
domain would be attached to the amino terminus of gp41
in the unprocessed form (termed gp160). Note that the
last ... splittingÕ in the TOCSY spectra of C5 indicates that
the trans isomer is the major isomer. Interestingly, the H
N
and H
a
line-widths of the region...
... 1ERT
(crystal structure of oxidized and reduced human thioredoxin, 1–105), 1DBY (NMR structure of thioredoxin in Chlamydomonas reinhardtii, 1–107),
1MEK (NMR structure of thioredoxin domain of protein- disulfide ... forms and
dissimilar intermolecular contacts near the active site in the
crystal, the conformation of the active site (-Cys-Gly-Pro-
Cys-) of...
... extracellular
domain. The second step will then be the deposition of
the ligand into the TM pocket causing the conformation
change of the receptor and triggering the coupling of the
receptor with the G protein ... protein in the intracellular domain s.
The first step of binding determines the ligand selectively
and the second step of binding is r...
... study,
which can be performed in solution, seems particularly
interesting in view of the fact that the interaction of water
with the putative ice-binding surface of TTTT in the single
crystal ... interactions, has been
reported very recently [24]. This study has allowed a
comparison of the hydrogen bonding between the protein in
water and the protein i...
... contains five Ig-like domains.
A comparison of the first three domains of DM64 and a
1
B-
glycoprotein shows that they are homologous to the three
DM43 domains [24]. Each of these domains in the ... a
1
B-glycoprotein, indicating the presence of
five immunoglobulin-like domains. DM64 neutralized both
the in vivo myotoxicity and the in vitro cytotoxicity...
... liver. Furthermore, we investigated the
speci®city of the human CBG with r espect to the glycone
and aglycone moieties, and in particular characterized the
ef®ciency of the enzyme in hydrolysing a ... (4NPGlc), incubating for 30 min at 37 °C, and
measuring the rel ease of 4NP.
Protein assays and protein sequencing
Total protein in crude and semipuri®e...