Báo cáo Y học: Puriﬁcation and characterization of novel cho

Báo cáo Y học: Puriﬁcation and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal anaerobic bacterium pptx

... the purification and characterization of chondroitin ABC and AC lyases, particulary chondroitin AC lyase, from an anaerobic bacterium (B. stercoris) in human intestine. The substrate specificity and ... Purification and characterization of novel chondroitin ABC and AC lyases from Bacteroides stercoris HJ-15, a human intestinal a...

Ngày tải lên : 31/03/2014, 23:20

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Báo cáo khoa học: Puriﬁcation and characterization of novel salt-active acharan sulfate lyase from Bacteroides stercoris HJ-15 ppt

... well as acharan sulfate (Table 5). The salt-active acharan sulfate lyase was the most active to acharan sulfate. When the salt-active acharan sulfate lyase activity for heparan was taken as 100%, ... Fr-b and Fr-c) containing the activity of these enzymes were collected separately and assayed for the activities degrading acharan sulfate and heparan sulfate. Fr-s had acharan sulfate l...

Ngày tải lên : 23/03/2014, 21:20

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Tài liệu Báo cáo Y học: Puriﬁcation, characterization, cloning, and expression of the chicken liver ecto-ATP-diphosphohydrolase pot

... Lewis-Carl,S.&Kirley,T.L.(1997)Immunolocalizationofthe ecto-ATPase and ecto-apyrase (ATPDase) in chicken gizzard and stomach: puriﬁcation and N-terminal sequence of the stomach ecto-apyrase. ... 7.4. On the other hand, the CaADPase activity is  80% of the MgADPase activity at a divalent ion-ADP concentration of 5 m M (data not shown). The ATPase and ADPase activities o...

Ngày tải lên : 22/02/2014, 04:20

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Tài liệu Báo cáo Y học: Puriﬁcation, characterization, immunolocalization and structural analysis of the abundant cytoplasmic b-amylase from Calystegia sepium (hedge bindweed) rhizomes ppt

... (Carlsbad, CA, USA). Preparation of speciﬁc antibodies against b-amylase and C. sepium RNase-related protein (CalsepRRP) Polyclonal antibodies were raised against b-amylase and the unglycosylated ... solvent accessible surfaces of the modelled amylase were calculated using the GRASP facilities. RESULTS Puriﬁcation and partial characterization of the b-amylase from C. sepium...

Ngày tải lên : 22/02/2014, 07:20

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Báo cáo Y học: Puriﬁcation and biochemical characterization of some of the properties of recombinant human kynureninase pptx

... Puriﬁcation and biochemical characterization of some of the properties of recombinant human kynureninase Harold A. Walsh and Nigel P. Botting School of Chemistry, University of St Andrews, ... metabolism. Rapid progress in the pathophysiology of human diseases has always been hampered by the availability of human tissue, aesthetics, and ethical considerations. T...

Ngày tải lên : 08/03/2014, 22:20

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Báo cáo Y học: Puriﬁcation and characterization of novel kininogens from spotted wolfﬁsh and Atlantic cod pdf

... biantennary and triantennary N-glycans that are terminated by a2 ,3-linked sialic acids, a high number of which are O-acetylated. About 1/3 of the glycans carry sulfate at N-acetylglucosamine units of ... water and analysed by MALDI-TOF MS. Phosphatase treatment Dry N-glycan samples were dissolved in 50 m M ammonium bicarbonate, and 1 U of calf intestinal alkaline phosphata...

Ngày tải lên : 08/03/2014, 23:20

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Báo cáo Y học: Puriﬁcation and characterization of three galactose speciﬁc lectins from Mulberry seeds (Morus sp.) ppt

... Puriﬁcation and characterization of three galactose speciﬁc lectins from Mulberry seeds ( Morus sp.) Tanzima Yeasmin, Md Abul Kashem Tang, Abdur Razzaque and Nurul Absar Department of Biochemistry, ... NI D-Galactose – 25 I N-Acetyl D-glucosamine 110 – NI Methyl -a- D-galactopyranoside – 30 I Methyl-b- D-galactopyranoside – 20 I MSL-3 N-Acetyl-galactosamine 110 – NI D-glucosami...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Puriﬁcation and characterization of VanXYC, a D,D-dipeptidase/D,D-carboxypeptidase in vancomycin-resistant Enterococcus gallinarum BM4174 docx

... polymerase (Stratagene) was used to amplify vanXY C usingpAT704as template with primers A (5¢-GCTA GGTCTCAATGAAC ACATTACAATT-3¢)andB(5¢-TATG GAATTCTCATG CGAACTGCCTCA-3¢) that included BsaIandEcoRI restriction ... the VanC phenotype, VanXY C must speciﬁc- ally hydrolyse D -Ala- D -Ala with minimal activity against D -Ala- D -Ser, a very different type of speciﬁcity. VanXY C , VanX-type...

Ngày tải lên : 18/03/2014, 01:20

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Báo cáo Y học: Puriﬁcation, characterization and subunits identiﬁcation of the diol dehydratase of Lactobacillus collinoides pot

... The enzyme required the adenosylcobalamin coenzyme for catalytic activity and the K m for the cofactor was 8 l M . Inactivation of the enzyme was observed by both glycerol and cyano- cobalamin. ... 1,2-ethanediol and glycerol to propionaldehyde, acetaldehyde and 3-hydroxy- propionaldehyde, respectively [1]. This dehydration reaction is the ﬁrst step of an anaerobic metabolism pa...

Ngày tải lên : 23/03/2014, 21:20

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Báo cáo Y học: Puriﬁcation and characterization of two secreted purple acid phosphatase isozymes from phosphate-starved tomato (Lycopersicon esculentum) cell cultures ppt

... various divalent metal cations and EDTA on the activity of SAP1 and SAP2. The standard assay A was used except that the phosphoenolpyruvate concentration was subsaturating (4 m M ). Enzyme activity ... kDa), BSA (66 kDa), ovalbumin (45 kDa), and carbonic anhydrase (29 kDa). AP was also visualized in acrylamide gels by staining for AP activity. SDS/PAGE was Fig. 1. Separation of se...

Ngày tải lên : 23/03/2014, 21:20

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