Báo cáo Y học: C-labelling experiments potx
Báo cáo Y học: C-labelling experiments potx
... N-ethoxycarbonyl amino acid ethyl esters and N-(N¢,N¢-dimethyl)methylene amino acid ethyl esters, were synthesized and analysed by GC-MS [4,13]. The mass spectra obtained from the GC-MS analysis ... phosphate pathway (PP pathway) constitutes a highly complex system that may seriously affect the estimation of the physiologically important flux through the oxidative PP pathway [7]. Considerable...
Ngày tải lên: 31/03/2014, 23:20
Tài liệu Báo cáo Y học: Structure of the O-polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3 potx
... f 2-amino-2-deoxygalactose and lysine. The D configuration of GalA and GalN was determined by GLC of the acetylated (S)-2-butyl glycosides and the L configuration of lysine by GLC of the acetylated (S)-2-butyl ... Shashkov 2 and Yuriy A. Knirel 2 1 Department of General Microbiology, Institute of Microbiology and Immunology, University of Lodz, Poland; 2 N.D. Zelinsky Institute of Organic Chemi...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: The insert within the catalytic domain of tripeptidyl-peptidase II is important for the formation of the active complex potx
... describe d in Materials a nd methods. Enzyme activity was a nalysed by the standard assay and the i mmunoreactivity was detected by Western blot analysis and quantitated as described i n Materials ... described previously usin g a ffinity purifi ed polyclonal chicken anti-(human TPP II) Ig [25]. The immunoreactivity was quantitated from scanned X-ray films by use of the MOLECULAR ANALYST softwar...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: CK2btes gene encodes a testis-specific isoform of the regulatory subunit of casein kinase 2 in Drosophila melanogaster potx
... radio- activity in this substrate. The addition of polylysine practically has n o effect on the phosphorylation of calmodulin by free a subu nit (Fig. 3, lane 4), but drastically stimulates activity of ... m M ). Fig. 3. Effect of polylysine on the phosphorylation of calmodulin by catalytic subunit or by holoenzyme reconstituted from CK2a and CK2btes proteins. Calmodulin was phosphorylated in...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Novel bradykinins and their precursor cDNAs from European yellow-bellied toad (Bombina variegata) skin potx
... This sensitivity is well within the capability of the ES-MS system employed. The primary structural diversity displayed within amphibian skin bradykinins may not simply be due to phylogenetic differences ... of each synthetic peptide was achieved by acid hydrolysis of a known gravimetric quantity of lyophilisate followed by amino acid analysis using an Applied Biosystems PTH-amino acid analys...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Steady-state kinetics of the glutaminase reaction of CTP synthase from Lactococcus lactis The role of the allosteric activator GTP in coupling between glutamine hydrolysis and CTP synthesis potx
... (enthalpo- gram) obtained for glutamine hydrolysis by CTP synthase. The complete hydrolysis of glutamine necessary for calcu- lating the molar enthalpy DH, and subsequently the rate of 4774 M. Willemoe ¨ s ... lactis enzyme, a plausible explanation may be that 4-phosphorylated UTP by itself acts as a weak activator of glutamine hydrolysis. This activation is greatly enhanced by GTP binding to...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Novel complexes of mammalian translation elongation factor eEF1AÆGDP with uncharged tRNA and aminoacyl-tRNA synthetase potx
... ousted by newly synthesized aminoacyl-tRNA (E) [32]. The finding that the complex of eEF1A, eEF1Ba and nonhydrolyzable analog of GTP could be dissociated by aminoacyl-tRNA rather than by deacylated ... °C. The carboxymethyl dextran matrix of the sensor chip was activated by a 30-lL injection of the mixture of 0.2 M 1-ethyl-3-[(3-dimethylamino)propyl]carbodiimide and 0.05 M N-hydroxysuccinimide...
Ngày tải lên: 21/02/2014, 15:20
Tài liệu Báo cáo Y học: Characterization of a novel silkworm (Bombyx mori ) phenol UDP-glucosyltransferase potx
... ith a specific enz yme activity. Keywords: Bombyx mori; detoxication; insect; UDP- glycosyltransferase. The UDP-glycosyltransferases ( UGTs) are a superfamily of enzymes that p lay a central role ... parental virus (vEGTSyngal+); BmUGT1, vSynBmUGT1; T, 48 h p.i. cells t reate d with tunicamycin. The mo lecular masses of g lycosy- lated and no nglycosylated BmUGT 1 a re in dicated by a rrows o...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: Kinetic study of sn-glycerol-1-phosphate dehydrogenase from the aerobic hyperthermophilic archaeon, Aeropyrum pernix K1 potx
... specificity and enzyme activity The substrate specificity of Gro1P dehydrogenase was examined using the purified enzyme. No activity w as observed t oward glyceraldehyde phosphate, Gro3P,glycer- ol-2-phosphate ... sn-glycerol-1-phosphate; Gro3P, sn-glycerol- 3-phosphate, Gro, glycerol. Enzymes: glycerol-3-phosphate dehydrogenase (NAD) (EC 1.1.1.8); glycerol de hydrogenase [NAD(P)] (EC 1.1.1.172)...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: Divergent members of a soybean (Glycine max L.) 4-coumarate:coenzyme A ligase gene family potx
... suitably ring-substituted cinnamoyl CoA esters as substrates (Fig. 6 ). However, phenyl ring modification involving hydroxylation and O-methylation can basically occur by different pathways, namely by modifications ... have been described, including cytochrome P450-dependent hydroxylations [11,21] as well as O-methylations that operate on caffeoyl-CoA and 5-hydroxyconiferyl aldehyde [12,22,23]....
Ngày tải lên: 22/02/2014, 04:20