Báo cáo y học: Identification of an element within the promoter of human selenoprotein P responsive to transforming growth factor-b ppt
... demonstrated an involvement of Smad 3 and 4 in transcriptional regulation of SeP by TGF-b 1 and we were able to identify the TGF-b -responsive element in the SeP promoter. Keywords: selenoprotein P; transforming ... Identification of an element within the promoter of human selenoprotein P responsive to transforming growth factor-b Volker Mo...
Ngày tải lên: 31/03/2014, 23:20
... Uppsala. The O-phosphopeptides GRRPsLFG and APDyTTPEMY-NH 2 were obtained from Dr A ˚ ke Engstro ¨ m at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and ... muscle. These results provide a new tool for studying eukaryotic histidine phosphorylation/dephosphorylation. Keywords: dephosphorylation; N-phosphorylation; phos- phoamidase; phosphope...
Ngày tải lên: 21/02/2014, 01:21
... sequencing of the ata cluster could provide an insight into the biosynthetic pathway of the other moieties of A201A and provide probes which may be useful to identify genes of the hygromycin A biosynthetic ... unit to the ketosynthetase domain of the PKSs for polyketide biosynthesis [35]. Therefore, AtaPKS1 could be an acyltransferase, which should be implicated...
Ngày tải lên: 21/02/2014, 01:21
Tài liệu Báo cáo Y học: Identification of syntaxin-1A sites of phosphorylation by casein kinase I and casein kinase II ppt
... deter- mined by C erenkov counting and the phosphopeptide fractions were analysed by ion-trap mass spectrometry. Mass spectrometry (MS) of phosphorylated peptides Ion-trap MS of in-gel digested phosphoprotein ... with trypsin and the peptides were separated by HPLC (data not shown). The mass spectrom etry of r adioactive peptides showed the presence of a doubly charged phospho...
Ngày tải lên: 22/02/2014, 04:20
Tài liệu Báo cáo Y học: Identification and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx
... Fig. 5, the addition of DNP or CCCP to exponential cultures led to an immediate arrest of growth followed by an increased expression of UP12. In the presence of DNP, the induction of UP12 was ... undergo a transition from a rapid growth phase to a stationary phase, which is accompanied by a variety of physiological changes that affect gene expression, the str...
Ngày tải lên: 22/02/2014, 07:20
Báo cáo Y học: Identification of the 19S regulatory particle subunits from the rice 26S proteasome potx
... in the lysylendopeptidase digestion buffer to digest into p eptides. The resultant peptides were collected and subjected to ESI-Q-TOF MS and MS/MS analyses. In th is analysis, the products of ... 000 and 110 000 D a. Identification of the rice RP subunit by ESI-Q-TOF mass spectrometry Prior to identification of the RP subunits by ESI-Q-TOF MS, we separated the subu...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf
... possibly a metal- dependent enzyme. Substrate specificity analysis To study the substrate specificity of the N-D-AAase protein, the activity of the enzyme against N-acyl- D -or L -amino acids and ... and D -tryptophan N-acetyl deri- vates and that there is chiral specificity. A comparison of N-acetyl- D -phenylalanine and N-chloroacetyl- D -phenylala- nine showed an increased a...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification of residues in the PXR ligand binding domain critical for species specific and constitutive activation docx
... genomic response elements in CYP3A4 and CYP3A7 as PXR [9,13]. PXR has been shown to bind phenobarbital response elements in the CYP2B gene promoter and to be a regulator of CYP2B10 [14] and CYP2B6 ... dramatically affects the basal activity of both the human and mouse receptors. MATERIALS AND METHODS Plasmid constructs, human PXR The full length cloning of human nucl...
Ngày tải lên: 08/03/2014, 16:20
Báo cáo Y học: Identification of residues critical for activity of the wound-induced leucine aminopeptidase (LAP-A) of tomato pptx
... nd porcine LAPs were noted [17]. Phe and Tyr in the P1 position of dipeptides promoted dipeptide cleavage by the porcine LAP and E. coli PepA, but reduced tomato LAP-A hydrolysis r ates by 65 and ... FEBS 2002 Lys and Gly slowed the hydrolysis rates of the tomato LAP-A, porcine LAP, and E. coli PepA markedly. An alysis of tripeptides showed that more diversity was tolerated i...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Identification of novel membrane proteins by searching for patterns in hydropathy profiles potx
... closely spaced triplet of hydropathy peaks bracketed by deep valleys. When applied to the human proteome the search procedure retrieved 153 profiles with a receptor-like triplet of peaks. The approach ... characteristic pattern of peaks in their hydropathy profiles. (A) The hydropathy profile of the human AChR alpha-1 subunit reveals a typical cluster of three peaks bra...
Ngày tải lên: 17/03/2014, 23:20