Báo cáo Y học: Kinetic studies of human tyrosyl-DNA phosphodiesterase, an enzyme in the topoisomerase I DNA repair pathway pot

... linking the active site tyrosine residue of topoisomerase I with the 3¢ terminus of DNA in topo- isomerase I DNA complexes which accumulate during treatment of cancer with camptothecin. In yeast, ... torsion- al strain of DNA during replication, repair and transcrip- tion processes by making single stranded breaks on DNA, unwinding and religating the DNA ends...

Ngày tải lên: 31/03/2014, 21:21

... competitive inhibitor against dihydroxyacetone phosphate and NAD(P)H. However, NAD(P) + acted as a competitive inhibitor against NAD(P)H and as a noncompetitive inhibitor against dihydroxyacetone ... distinct features from eubacteria and eukaryotes, including the unique stereo- chemical backbones of phospholipids in their cellular membrane. The core lipid of the phospholipids and...

Ngày tải lên: 22/02/2014, 04:20

... (rutin) Quercetin-4¢-GlA Kaempferol-3-Glc Isorhamnetin-3-Glc Flavanones Naringenin-7-GlcRha (naringin) Hesperetin-7-GlcRha (hesperidin) Dihydrochalcones Phloretin-7-Glc (phlorizin) Secoiridoids Oleuropein Anthocyanidins Cyanidin-3-Glc ... similarity and 83.6 % ami no- acid similarity with gu inea pig C BG, and showed homol- ogy w ith domains III and I V o f mammalian LPH (56 and 57% amino-a...

Ngày tải lên: 08/03/2014, 16:20

... consistent with titration of the catalytic histidine, H438(440). The persistence of activity in the presence of the protonated form of the catalytic histidine is inconsistent with the generally ... catalytic site (i. e. a catalytic triad of Ser-His-Glu, an oxyanion hole, a p-cation-binding site, and an acyl-binding pocket) are the same in AChE and BuChE (Fig. 1...

Ngày tải lên: 20/02/2014, 23:20

... cells is the same size as the polypeptide identified in crude extracts of spinach leaves by antibodies raised against the recombinant protein. The size of this protein is very similar to t hat of ... for Fru-2,6-P 2 ase activities in the liver isoform of t he mamma- lian enzyme are conserved in the same relative positions within the spinach leaf sequence (Fig. 1)....

Ngày tải lên: 22/02/2014, 04:20

... rod linker, which resulted in the disintegration of the rod complexes. The two bilins present in b-phycocyanin give a greater contribution to the UV absorption than the s ingle bilin of the other ... components, and the individual biliproteins, either with or without attached linkers, are obtained [6]. The relative stability of biliprotein- linker complexes varies am...

Ngày tải lên: 08/03/2014, 16:20

... first obvious difference in their catalytic properties to be identified. Kinetic data were consistent with the inhibition due to binding of the second substrate. We discuss the inhibition mechanism ... contribu- tion of the aliphatic chain of fatty acids to their binding, as compared with P450BM3. In other words, the interaction of P450foxy with the carboxylate anion o...

Ngày tải lên: 08/03/2014, 22:20

... sensitivities found in our study were higher in comparison to the study in question but specificity is lower. This situation may be related with the phase of the brucella infection. In a study ... agglutination tests have an important role in the diagnosis of brucellosis. Main antigenic structure which is imported in the diagnosis of bru- cellosis is the s...

Ngày tải lên: 25/10/2012, 10:56

... currently an associate professor in Department of Biochemistry and Nutrition in Iran University of Medical Sciences. He is interested in study of nutrition and trace elements in human blood. Manuchehr ... Imamian obtained BSc in Chemistry from Payam-Nour University of IRAN in 1996 and MSc in Biochemistry from Iran University of Medical Sciences in 2001. His a...

Ngày tải lên: 03/11/2012, 09:49

... iron and lipids [14,56,57] may be relevant in a variety of liver diseases. It is anticipated that iron overload in hepatic cells in clinical situations in vivo might cause changes in lipid metabolism and ... accumulation and (c) increased the prooxidant state and the level of oxidatively modified proteins. These results are in accordance with previous in vitro and in vi...

Ngày tải lên: 19/02/2014, 16:20