Báo cáo Y học: Antiproliferative proteins of the BTG/Tob family are degraded by the ubiquitin-proteasome system docx

... Antiproliferative proteins of the BTG/Tob family are degraded by the ubiquitin-proteasome system Hitoshi Sasajima, Koji Nakagawa and Hideyoshi Yokosawa Department of Biochemistry, Graduate ... of BTG/Tob family proteins are situated on the conserved N-terminal BTG/Tob homology domains, because BTG1, BTG2, Tob, and Tob2 proteins are all degraded b...

Ngày tải lên: 31/03/2014, 21:21

... decreased dramatically, which seemed to be caused by irreversible denaturation of the enzyme. With the exception of the temperature- activity profile, the characteristics of the enzyme were determined ... specificity and enzyme activity The substrate specificity of Gro1P dehydrogenase was examined using the purified enzyme. No activity w as observed t oward glyceraldehyde...

Ngày tải lên: 22/02/2014, 04:20

... analyzed by SDS/ PAGE. Therefore, we focused on the purification and study of the rBla g 1 expressed in methanol-induced Pichia. Origin of expression of rBla g 1 multiple forms The expression of ... fractions from another culture grown under the same conditions. (B) ELISA activity of rBla g 1 expressed by meth- anol-induced P. pastoris and purified by affinity chromatograp...

Ngày tải lên: 08/03/2014, 23:20

... the functional core of the MREs and the Sp1 binding element, and the mutated bases are indicated by italic type). Western blot analysis The MREa-binding proteins purified from HepG2 cells by the ... the export of copper from the cytosol, similar to the P-type ATPase [14]. On the basis of the fact that MREs mediate the transcriptional response of theMTgen...

Ngày tải lên: 17/03/2014, 23:20

... identified in humans [15, 18]. Most of them are located near the N-terminal portion of the proteins followed by zinc-fingers, and are involved in the reg- ulation of cell division and differentiation ... over the progression of cancer; and the second question is whether the PR domain alone possesses anticancer activity. In this study, we quan- titatively analyz...

Ngày tải lên: 25/10/2012, 11:15

... During the therapeutic process, real-time estimation of the therapeutic effect was carried out by the computer system through the graphic changes in the target field and the hyperecho of the ... the care and use of laboratory animals from the Ministry of Science and Technology of the People’s Republic of China. Instruments HIFU ablation was performed...

Ngày tải lên: 25/10/2012, 11:18

... 3 days of culture, the medium was refreshed, and then the medium changed every other day. Cell passaging was per- formed when cell confluence reached 85%. The purity and immunophenotype of ... decompression surgery, etc. The efficacy of these strategies is inconsistent and they have dis- advantages of difficult manipulation. In addition, surgery may cause new trauma and in...

Ngày tải lên: 25/10/2012, 11:18

... cytopenias. The use of these agents is costly and has not been studied rigorously in a randomized controlled format to assess their value and impact on efficacy of therapy although they are ... with the latest combination therapy. However, existing data support therapy of the patient with compensated cirrhosis. Cirrhotic patients may tolerate therapy less well, given the...

Ngày tải lên: 31/10/2012, 17:11

... proteins implicated in the biosynthesis of the aminonucleoside moiety of A201A. Here we report the sequencing of a total of 6946 bp, which include the ata genes most likely implicated in the biosynthesis of this ... be related to their counterparts of the puromycin and hygromycin A biosynthetic pathways, respectively. The puromycin biosynthetic gene cluster (pur)fr...

Ngày tải lên: 21/02/2014, 01:21

... as the high- affinity peak of wild-type LPL. The LPL in the eluate was detected by Western blot with LPL polyclonal antibody 7640. The fraction number of the high-affinity peak was the same for the ... orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other,...

Ngày tải lên: 21/02/2014, 15:20