Báo cáo Y học: Purification and characterization of the thyrotropin-releasing hormone (TRH)-degrading serum enzyme and its identification as a product of liver origin doc

... derivatives as indicated in the table. The molar masses and the absolute amounts of the inhibitors and the maltodextrins added to the filter paper discs are indicated. The size of the zones of growth ... to about half the area of the total cross-section [6]. Removal of the globular domain may increase the flexibility of loop 4 and other surface expose...

Ngày tải lên: 31/03/2014, 08:20

... they are also known as the E-NTPDases. Ecto-ATP-diphosphohydrolase (ecto-ATPDases) and ecto-ATPases are two different subfamilies of the E-ATPases. E-type ATPases are ATPases that have similar ... Lewis-Carl,S.&Kirley,T.L.(1997)Immunolocalizationofthe ecto-ATPase and ecto-apyrase (ATPDase) in chicken gizzard and stomach: purification and N-terminal sequence of the stom...

Ngày tải lên: 22/02/2014, 04:20

... soybean b-amylase (Cys82, Cys97, Cys208 and Cys343). On the analogy of the soybean b-amylase, the active site of the C. sepium b-amylase most probably consists of a cleft located between the barrel ... against b-amylase and C. sepium RNase-related protein (CalsepRRP) Polyclonal antibodies were raised against b-amylase and the unglycosylated isoform of CalsepRRP [14...

Ngày tải lên: 22/02/2014, 07:20

... neuroprotection; quinolinic acid; tryptophan metabolism. Rapid progress in the pathophysiology of human diseases has always been hampered by the availability of human tissue, aesthetics, and ethical considerations. The ... strong anionic and cationic sepa- rations. The purity of the enzyme was determined by SDS/ PAGE, and the molecular mass verified by MALDI-TOF MS....

Ngày tải lên: 08/03/2014, 22:20

... assay After each chromatographic step the inhibitory activity of collected fractions was determined using papain as the enzyme in the assay [6]. The same assay was used to determine inhibitory activites ... kininogen carries biantennary and triantennary N-glycans that are terminated by a2 ,3-linked sialic acids, a high number of which are O-acetylated. About 1/3 of the...

Ngày tải lên: 08/03/2014, 23:20

... buffer containing 0.2 M galactose. Protein and carbohydrate analysis The concentration of protein was measured by the method of Lowry et al. [13] using BSA as the standard. The presence of sugar in the ... Purification and characterization of three galactose specific lectins from Mulberry seeds ( Morus sp.) Tanzima Yeasmin, Md Abul Kashem Tang, Abdur Razzaque and N...

Ngày tải lên: 18/03/2014, 01:20

... polymerase (Stratagene) was used to amplify vanXY C usingpAT70 4as template with primers A (5¢-GCTA GGTCTCAATGAAC ACATTACAATT-3¢)andB(5¢-TATG GAATTCTCATG CGAACTGCCTCA-3¢) that included BsaIandEcoRI restriction ... bi[tris(hydroxymethyl)-meth- ylamino] propane and 10 lL10m MD -Ala- D -Ala, incuba- ted at 37 °C for 30 min, and assayed for D , D -dipeptidase activity using the D -amino...

Ngày tải lên: 18/03/2014, 01:20

... enzyme required the adenosylcobalamin coenzyme for catalytic activity and the K m for the cofactor was 8 l M . Inactivation of the enzyme was observed by both glycerol and cyano- cobalamin. The ... °C. Dehydratase assays Two methods were used for the determination of the activity. During the purification and characterization enzyme steps, the assay was car...

Ngày tải lên: 23/03/2014, 21:20

... SE of n ¼ 3 separate determinations. Table 2. Effect of various divalent metal cations and EDTA on the activity of SAP1 and SAP2. The standard assay A was used except that the phosphoenolpyruvate ... the hydrolysis of 1 lmol of substrate per min at 25 °C. Phosphatase assay A For routine measurements of AP activity, the hydrolysis of phosphoenolpyruvate to pyruva...

Ngày tải lên: 23/03/2014, 21:20

... °C. Synthesis of XAC-agarose gel The ligand af®nity gel was prepared based on the method described by Nakata [15]. XAC was dissolved in dimethyl- sulfoxide at a con centration of 0 .5 mgámL )1 .Af®-Gel10 resin ... N-terminal protein sequencer (Procise 494, Applied Biosystems). Protein assay and amino-acid analysis Protein concentrations were determined by the Amido black assay [...

Ngày tải lên: 24/03/2014, 00:21