Báo cáo Y học: Solution structure of a hydrophobic analogue of the winter flounder antifreeze protein doc

... strongly hydrophobic side chains; this feature has been aptly described by Rajan et al. as a Teflon coating that can surround a helix [16] in the case of a mixture of water and hexafluoroacetone hydrate, ... was adopted for the elution of the peptide. The fraction containing the pure peptide was lyophilized twice and the purity assessed by a MALDI-TOF analysis using...

Ngày tải lên: 08/03/2014, 09:20

... chemical shift changes were Table 1. Sequence alignment of TTTT and VVVV2KE. 1 2 13 24 35 TTTT D TASDAAAAAAL TAANAKAAAEL TAANAAAAAAA TAR VVVV2KE D VASDAKAAAEL VAANAKAAAEL VAANAKAAAEA VARCONH 2 1260 ... carboxyl group of Asp1 and the backbone amide of Ser4. The C-terminal cap structure, however, makes use of the Arg37 side chain to form a hydrogen bond to the backbone carbon...

Ngày tải lên: 31/03/2014, 21:21

... set of solutions reflects any uncertainty in the calibration as well as alternative fits to the set of distances. The main advantages of the procedure are simple: the details of the calibration are ... of theoretical cross relaxation rates. These values may be replaced by scaled experimental values and the logarithm of the matrix may be taken to obtain distances that...

Ngày tải lên: 22/02/2014, 07:20

... shown by Fig. 5A, the amino terminal end of the C5 domain would be attached to remaining domains of gp120 in the native structure. The carboxy terminal end of the C5 domain would be attached to the ... helix. The tertiary structure of HIV gp120 C5 was determined by an iterative method of torsion angle molecular dynam- ics followed by simulated annealing [18]. A su...

Ngày tải lên: 08/03/2014, 16:20

... inhibitor of the sodium pump, has been identified in blood plasma, adrenal glands, and the hypothalamus of mammals. The adrenal gland as a source of ouabain The surprising observation that a plant toxin ... Ouabain is also taken up by cultured adrenal cells [28]. Consistent with the adrenal gland being a major place of synthesis and/or storage of ouabain, adrenalectom...

Ngày tải lên: 24/03/2014, 00:21

... wild- type protein [20]. A quantitative analysis of the ribonuclease activity of a- sarcin has been developed by using the dinucleotide ApA as substrate [18]. Both mutant variants hydrolysed ApA. They ... connectivity of the secondary structure elements in the three proteins. The catalytic mechanisms of a- sarcin and RNase T1 are similar [18–20] and related to that...

Ngày tải lên: 31/03/2014, 23:20

... secondary structure are shown; b-strands are represented by arrows and the a- helix by a spiral. Table 1A. NMR structural calculations summary: restraints used in the structure calculation, and type ... del Pozo A & Gavilanes JG (2001) RNase U2 and alpha- sarcin: a study of relationships. Meth Enzymol 341, 335–351. 3 Lacadena J, A ´ lvarez-Garcı ´ a E, Carreras-Sangra...

Ngày tải lên: 18/02/2014, 08:20

... as a type-I. The +2x, )1 topology [47] of the triple-stranded antiparallel b-sheet was Fig. 3. Summary of data used in the determination of the secondary structure of crotamine. (A) Primary sequence ... Biochemistry, University of Parma, Italy; 2 Centro Interfacolta ´ Misure, University of Parma, Italy; 3 Department of Biochemistry and Immunology, University of Sa...

Ngày tải lên: 20/02/2014, 11:20

... hTRXL-N may account for the formation of a monomer, instead of a dimer in the case of TRX. Furthermore, the loss of intermolecular disulfide-bonds and the disbandment of the hydrophobic patch may also ... Zhou 2 , Mark Bartlam 1 , Qing Guo 1 , Yiwei Liu 1 , Yixin Sun 1 , Yu Gao 1,2 , Sheng Ye 1 , Guangtao Li 2 , Zihe Rao 1 , Boqin Qiang 2 and Jiangang Yuan 2 1 Laborat...

Ngày tải lên: 08/03/2014, 22:20

... properties requires structure and dynamic characterization in solution of the various functionally relevant states. To achieve this aim, we initiated a structural and dynamics analysis of Ca 2+ -saturated NSCP ... by x-ray crys- tallography. J Mol Biol 223, 601–606. 16 McPhalen CA, Sielecki AR, Santarsiero BD & James MN (1994) Refined crystal structure of rat parvalbumin,...

Ngày tải lên: 23/03/2014, 13:20