Báo cáo Y học: Puri®cation and properties of an alkaline proteinase of Fusarium culmorum doc

... [6]. LipA demonstrates hydrolytic activity toward emulsions of both medium and long chain triacylglycerols (Fig. 3). Areas of olive oil and tricaprylin hydrolysis are clearly seen and correspond with ... sp.RAG-1;protein purification; zymogram. Lipases are glycerol ester hydrolases (EC 3.1.1.3) that catalyze the hydrolysis of triacylglycerols to free fatty acids and glycerol. The...

Ngày tải lên: 23/03/2014, 21:20

... 807 Puri®cation and properties of an alkaline proteinase of Fusarium culmorum Anja I. Pekkarinen 1,2 , Berne L. Jones 3 and Marja-Leena Niku-Paavola 2 1 Department of Agronomy, University of Wisconsin-Madison, ... espe- cially in Europe [1]. FHB causes severe yield losses in w heat and barley and reduces the crop quality by destroying some of the necessary grai...

Ngày tải lên: 31/03/2014, 21:21

... enzyme which catalyses the b,c- hydrolytic cleavage of the amino acids L -kynurenine (1 )and L -3-hydroxykynurenine (2)togive L -alanine (3) and either anthranilic acid (4) or 3-hydroxyanthranilic ... Purification and biochemical characterization of some of the properties of recombinant human kynureninase Harold A. Walsh and Nigel P. Botting School of Chemistry, University...

Ngày tải lên: 08/03/2014, 22:20

... considerably smaller than that of ÔstandardÕ [NiFe] hydrogenases and contains only the cysteine ligands for the proximal [4Fe)4S]cluster.The large and small subunits of the membrane-bound hydro- genases ... of a membrane-bound enzyme complex from C. hydro- genoformans composed of a hydrogenase and a CO dehydrogenases. The complex catalyzes the conversion of CO to CO 2 and H...

Ngày tải lên: 23/03/2014, 21:20

... (1999) Purification and characterization of S-adenosyl- L -methionine: desoxyhemi gossypol-6-O-methyltransferase from cotton plants. An enzyme capable of methylating the defense terpenoids of cotton. Plant Physiol. ... ascertained. Keywords: S-adenosyl- L -methionine:flavonoid 4¢-O-methyl- transferase; O-methyltransferase; Fusarium oxysporum f. sp. dianthi; Dianthus caryophyllus; ca...

Ngày tải lên: 08/03/2014, 08:20

... (PerSeptive Biosystems, Framingham MA, USA) tip, and a sample of the flow- through carrying the glycans was drop-dialysed and analysed by MALDI-TOF MS. RESULTS Isolation of cysteine proteinase inhibitors The ... a2,3-sialylated biantennary and triantennary N-glycans, with extensive sialic acid O-acetylation. Also O-glycosidic glycans were recovered from wolffish kininogen. The perme...

Ngày tải lên: 08/03/2014, 23:20

... lymphocyte transformation, inactivation of certain types of tumor cells and precipitation of certain polysaccharides and glycoproteins [1,2]. Plant lectins isolated from a wide variety of plants ... methyl-b-D-galactopyrano- side, lactose and raffinose. The lectins MSL-1, MSL-2 and MSL-3 contained 5.7, 5.4 and 4.5% neutral sugars, respec- tively, and the sugar composition...

Ngày tải lên: 18/03/2014, 01:20

... mutagenesis [20], and later confirmed by analysis of the crystal structure [19]. Comparison of the active site of VanXY C with VanX- and VanY-type enzymes indicated that all of these enzymes contained ... standards; lane 2, cytoplasm containing MBP-VanXY C ;Lane3,MBP–VanXY C after amylose resin chroma- tography. (C) Purification of His 6 –VanXY C . Lane 1, molecular mass standards...

Ngày tải lên: 18/03/2014, 01:20

... purification of turtle liver GSTs using Matrix Red dye ligand chromatography, Sephacryl S-200 gel filtration and hydroxylapatite ion exchange chromatography. GST activity from liver of both aerobic and anoxic ... several forms and, hence, this study of turtle liver GST was undertaken to identify any anoxia-responsive changes in isozymic forms, specific activities, and kinetic prope...

Ngày tải lên: 23/03/2014, 15:20

... competitive. Inhibition of SAP1 and SAP2 by molybdate and fluoride was competitive and mixed, respectively (Table 4). More- over, I 50 and K i values of SAP2 for molybdate, fluoride, and P i were generally lower than ... slices containing 3 lgofSAP1(lane1)andSAP2(lane2)andanalyzedon an SDS/14% PAGE minigel as previously described [40]. Peptides were stained with SYPRO Red and...

Ngày tải lên: 23/03/2014, 21:20