Báo cáo khoa học: "The detection and representation of ambiguities of intension and description" pptx

... The detection and representation of ambiguities of intension and description Brenda Fawcett and Graeme Hirst Department of Computer Science University of Toronto Toronto, ... the beliefs of external agents. We propose that a unified approach to the representation of the alternative readings of intension- related ambiguities can be based on the notion of a descriptor ... evaluated with respect to intensionality, the beliefs of agents, and a time of application. We describe such a representation, built on a standard modal logic, and show how it may be used...

Báo cáo khoa học: The N-terminal hybrid binding domain of RNase HI from Thermotoga maritima is important for substrate binding and Mg2+-dependent activity pot

... of 1mm MgCl2 and MnCl2were 410-fold lower and 1.6-fold higher than those of Tma-RNase HI. The Kmvalues of Tma-CD determined in the presence of 1 mmMgCl2 and MnCl2were 5.1- and 6.8-fold ... partial sequences of D13-R4-D12 (C) and R9-D9 (E) are indicated along thegel. (B, D, F) Schematic representation of the sites and extents of cleavage by various RNases H. Cleavage sites of R12 ⁄ D12 ... puriﬁed and biochemically characterized. Tma-RNase HI prefersMg2+to Mn2+for activity, and speciﬁcally loses most of the Mg2+-depen-dent activity on removal of the HBD and 87% of it by...

Báo cáo khoa học: The structural basis for catalytic function of GMD and RMD, two closely related enzymes from the GDP-D-rhamnose biosynthesis pathway pdf

... RMD monomer. The cofactor-binding domain and the substrate-binding domain are shown in aqua and light sand, respectively; the APPR portion of the cofactor (dark gray) and the ligand analog GDP-D-Man ... integration of the anomeric signals, the4-keto and gem-diol forms of this compound coexist inequilibrium at an approximately 5 : 2 ratio. Fullassignment of the NMR spectra of compounds B and C and ... toassign C4 of compounds B and C. It is of importancethat a signal corresponding to C4 of compound B wasobserved at dC= 208.8 p.p.m. and was indicative of acarbonyl group, whereas that of compound...

Báo cáo khoa học: The carbohydrate-binding module family 20 – diversity, structure, and function doc

... bioengineering using SBDs. One of the veryobvious possibilities is the engineering of the size and properties of starch granules by overexpression of isolated SBDs, tandem repeats thereof, or SBD fusionswith ... metabolism and structural characterization, and investigation of the thermodynamic ﬁngerprint of arepresentative from this SBD group would advanceour understanding of their mode of action. ... enzymes of GH13, GH14, and GH15, the plant GWD3 [18], and the mammalianproteins laforin (EC 3.1.3.16/48) [39] and genethonin-1[40]. CBM21s from GH13 and GH15 and regulatory su-bunits of protein...

Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx

... 177 and is involved in stabilizing strands b8 and b9.Ca4 is the weak binding site in PRK, which is formed bythe side chain of Asp260 and the carbonyl oxygen of Thr16, and stabilizes the N- and ... VPRK and SPRK have aninsert of three residues. Ca3 is the so-called strong bind-ing site found in PRK and formed by the side chain of Asp200 and the carbonyl oxygen atoms of residues 175 and ... coordinated to the carboxyl oxygen atoms of the side chains of Asp11, Asp14 and Asp21, the amideoxygen atom of Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig. 3) in an arrangement similarto...

Tài liệu Báo cáo khóa học: The PAS fold A redeﬁnition of the PAS domain based upon structural prediction ppt

... regulators of responses to hypoxia [16] and embryologicaldevelopment of the central nervous system [17]. Many PASdomains bind cofactors or ligands, which are required forthe detection of sensory ... binding of oxygen and other strong-ﬁeldligands. The heme domain permits kinase activity in theabsence of bound ligand, but when the appropriateexogenous ligand is bound, this domain turns off ... E. coli, A. vin-elandii and C. elegans, to analyse their complement of PASdomains. These species have been studied extensively and many details of their gene expression and function areknown.The...

Báo cáo khoa học: The product chain length determination mechanism of type II geranylgeranyl diphosphate synthase requires subunit interaction pptx

... wild-type and mutant P. ananatisGGPSs contained, in a ﬁnal volume of 200 lL, 0.5 nmol of [1-14C]IPP (2 GBqÆmmol)1), 0.5 nmol of an allylic primer(GPP, FPP or GGPP), 1 lmol of MgCl2,2lmol of ... made with type 1 FPSs from human [11] and pro-tozoa [6,12], and some of the structures [e.g. FPS fromhuman (2F94 and 3B7L) and Trypanosoma brucei(2P1C and 2I19) binding bisphosphonate inhibitorswith ... center of the geranyl-geranyl-chain and bends it towards the bottom of thecavity formed by L185 in a-helix E, I216 in a-helix F, and D182¢ and S186¢ in a-helix E¢ (Fig. 8B, left). Thefourth and...

Báo cáo khoa học: The three typical aspartic proteinase genes of Arabidopsis thaliana are differentially expressed docx

... areexpressed in many cells of the seeds and developing seedpods. The A1 and A3 genes are expressed in the sepals and petals of ﬂowers as well as the outer layer of the style, but arenot expressed ... goal of understanding their function inthe whole plant. Aspartic proteinases (EC 3.4.23) are one of the major classes of proteolytic enzymes found in plant and animal viruses, microbes and plant ... The appearance of the aspartic proteinase in the seivecells of barley stems [6], in degenerating lily petals [7] and senescing leaves of B. napus [5] suggest an expanded role of these enzymes...

Báo cáo khoa học: The consensus motif for N-myristoylation of plant proteins in a wheat germ cell-free translation system ppt

... N-myristoylation of GFP and DHFR fused to a myri-stoylation consensus motif. (A) Structures and N-terminal aminoacid sequences of wild-type (wt) and G2A mutant forms of Myr–GFP and Myr–DHFR. ... of the expected size. The positions of pro-tein size standards are shown on the left of the gel.Table 1. Observed and calculated mass values for the trypticN-terminal peptides of ARF1A1c and ... set of empirical rules for a myristoylation motif has beenproposed on the basis of the structure of Saccharomy-ces cerevisiae NMT and of in vitro kinetic analysis of the puriﬁed protein and...

Báo cáo khoa học: The cellular oxygen tension regulates expression of the endoplasmic oxidoreductase ERO1-La ppt

... that the expression of the rat,mouse and human homologues of ERO1-Like protein a butnot of the isoform ERO1-Lb are stimulated by hypoxia inrats vivo and in rat, mouse and human cell cultures. ... meaning of the oxygen regulation of ERO1-Laexpression likely is to maintain the transfer rate of oxidizingequivalents to PDI in situations of an altered cellular redoxstate induced by changes of ... theNational Institutes of Health Guide for the Use of Laboratory Animals and the German Law on the protec-tion of Animals. Male Sprague–Dawley rats (200–250 g)that had free access to food and water were...

Xem thêm