Báo cáo khoa học: Chaperone and antichaperone activities of trigger factor pdf

... of trigger factor, as was found earlier for trigger factor assisted refolding of GAPDH [24], increase with increasing concentrations of trigger factor. The t 1/2 of Fig. 2. Effect of trigger factor ... presence of CyP. Ó FEBS 2002 Chaperone and antichaperone activities of trigger factor (Eur. J. Biochem. 269) 4519 effect of the solution conditions on f...

Ngày tải lên: 31/03/2014, 09:21

... result of the presence of negatively-charged residues at P2 and P2¢ that are not accommodated within the b2-tryptase active site because of the presence of Asp147 and Asp143 within the S2 and the ... specificity of CG and chymase Crystallographic data show that the S2¢ subsite of CG is highly polar as a result of the presence of three posi- tively-charged residues: Arg4...

Ngày tải lên: 28/03/2014, 22:20

... Department of Biology and Biochemistry, University of Bath, UK 2 Department of Biochemistry, University of Wisconsin–Madison, USA 3 Department of Chemistry, University of Wisconsin–Madison, USA Introduction Upon ... R h F o , where F o and F c are the observed and calculated structure factor amplitudes of reflection h, respectively. R free is equal to R cryst for a random...

Ngày tải lên: 14/02/2014, 22:20

... structure of cN-II showing interfaces A and B and the Mg 2+ site. The inset shows the tertiary structure of each subunit. Effector sites 1 and 2 and the active site are shown. Table 2. Effect of point ... transfer of phosphate esterified in the 5¢ position of 6-hydroxypu- rine monophosphate nucleosides [1]. The transfer of phosphate can lead to phosphorylation of inosine,...

Ngày tải lên: 15/02/2014, 01:20

... HPRT and hypoxanthine. (C) PRTFDC1 and guanine. (D) HPRT and guanine. Table 2. K m and V max values for Hx and G in the presence of 1 mM PRPP, determined using the DE-81 filter paper assay and ... characterization of numerous com- plexes of the human HPRT and several bacterial and protozoan HPRTs have been undertaken [13–17]. The structure of human HPRT can be divided i...

Ngày tải lên: 15/02/2014, 01:20

... performed using T-COFFEE [44]. Docking simulation of substrate on the active site of HYD Js The substrate binding pockets of HYD Js and the mode of interaction between substrate and enzyme were ... [39]. Cloning and expression of putative HYDs The genomic DNA of Jannaschia sp. CCS1 and Pseudomo- nas fluorescens PfO-1 were kindly supplied by Mary Moran (University of Georg...

Ngày tải lên: 18/02/2014, 08:20

... an accurate measure of the k off parameter in CaM-bound eNOS and nNOS [58], and thus prevented assessment of the relative importance of conformational change rates versus rates of FMNH 2 formation ... Model and simulations of cytochrome c reduction by NOS enzymes. (A) Scheme of cytochrome c reduction. The model uses four kinetic rates: dissociation (k 1 ) and association (...

Ngày tải lên: 18/02/2014, 11:20

... & Buckmann AF (1989) Enzymatic-synthesis of 4R-H-2NAD(P)H and 4S-H-2NAD(P)H and determination of the stereo- specificity of 7-alpha-hydroxysteroid and 12-alpha-hy- droxysteroid dehydrogenase. ... isotope effects using PEG-modified variants and glycoforms of glucose oxidase: the relationship of modification of the protein envelope to C–H activation and tunneling. Biochem...

Ngày tải lên: 18/02/2014, 11:20

... and pyridine nucleotides is highly dependent on the approach and colinear orientation of the N5 of the flavin, the hydride to be transferred, and C4 of the nicotinamide. In FNR, displacement of ... mutagen- esis of tyrosine-98 in the flavodoxin from Desulfovib- rio vulgaris (Hildenborough): regulation of oxidation– reduction properties of the bound FMN cofactor by aromatic,...

Ngày tải lên: 18/02/2014, 11:20

... D and E + F), (b) a few hydrogen bonds, and a hydrophobic interaction between A + C, B + E and D + F, and (c) electrostatic forces in the central channel of the hexamer between B, C and F, and ... Phe133 of UpUMPK was mutated to Asn or Ala. The mutant enzymes, F133N and F133A, were expressed, purified and characterized. With 1 mm UMP and ATP as substrates, the activities...

Ngày tải lên: 18/02/2014, 16:20