Báo cáo khoa học: Redox-sensitive loops D and E regulate NADP(H) binding in domain III and domain I–domain III interactions in proton-translocating Escherichia coli transhydrogenase potx
Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc
... cleavages generated in vitro occurring mainly within domain III of ETA-A. Cell-free endosomes preloaded in vivo with ETA intraluminally processed and extraluminally released intact ETA and ETA-A in vitro ... cathepsins B and D degraded ETA in a pH-dependent manner, with maximal degradation being observed at pH 4. The ETA fragments generated by the pure cathepsins (especial...
Ngày tải lên: 18/02/2014, 04:20
Báo cáo khoa học: Structure–function relationship of novel X4 HIV-1 entry inhibitors – L- and D-arginine peptide-aminoglycoside conjugates pptx
... that Neam-r9 and Neo-r9 may interfere with the binding of the viral envelope to the cell. D- arginine (6- and 9-mers) peptide- aminoglycosides are readily internalized and concentrated in the cell ... to the desired compound. Solvents were removed under reduced pressure, and extracted three times with warm ethyl acetate. The ethyl acetate layer was washed twice with water, dried...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redefinition of the I-conotoxin superfamily pot
... electrodes were located in wells 1 and 2, and a ground electrode in well 2. Recording electrodes were located in wells 3 and 4. Peptide was added by exchange of the fluid contents of well 4. To eliminate ... d- phenylalanine residue in r11a is underlined). There is a notable difference in the sequence diver- gence from r11a at the C-terminal 12 amino acids (boxed residues in...
Ngày tải lên: 16/03/2014, 22:20
Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt
... ligand is engulfed in the cleft and held in place by extensive hydrogen-bonding inter- actions with residues in the L -domain (residues N204, D2 05, N231, E2 56 and E2 90) and the S -domain (resi- dues ... studies by demonstrating that the binding of Glc at the active site indeed induces a large-scale domain move- ment that closes the active site cleft and creates the s...
Ngày tải lên: 30/03/2014, 04:20
Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"
... is further confirmed by the fact that there were no more medications ordered or diagnostic tests interpreted in the traditional schedule per patient-day, and there were in fact fewer procedures performed ... other studies have suggested [8,9]. Despite these relatively minor limitations, these two studies offer the best evidence to date that sleepy interns provide bad patient care, and...
Ngày tải lên: 25/10/2012, 10:45
Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx
... and p25aDC were incubated with the homogenate and treated like B-p25a, except they were detected with using p25a-1 antibody. Representative data from one of three independent experiments are ... cloned into pET1 1d vector, expressed in E. coli and purified. The purified proteins were subjected to reducing SDS ⁄ PAGE and Coomassie blue staining. Molecular size markers are shown on...
Ngày tải lên: 14/02/2014, 21:20
Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf
... the enzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivase complexes (upper and lower) appeared above them (Fig. 5A, lane c). When apoenzyme was used ... the reactivase eluted separately at their respective retention times. In contrast, a peak of the free reactivase decreased and a new peak appeared in the presence of ADP. The latter p...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf
... the predicted target genes are in large quantity and most have not been validated experimentally. Therefore, we used a cDNA microarray to search for downregulated genes in gastric adenocarcinoma ... tissue samples. The genes that were predicted by two of the algorithm programs (pictar and targetscan release 5.1) and were also downregulated in our cDNA microarray were selected as c...
Ngày tải lên: 18/02/2014, 04:20
Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding specificity and target candidates doc
... measured. For each assay, at least three independent yeast colonies were used. Direct interaction between APUM and RNA in the yeast three-hybrid system To investigate direct interaction of the ... with Klenow fragment of Escherichia coli DNA polymerase. After extension, the double-stranded DNA were digested with XbaI and SmaI and ligated to an AvrII-SmaI-digested pRH5¢ vector. The...
Ngày tải lên: 18/02/2014, 06:20
Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
... similar central structured region connected by loops of different length. Indeed, extended loops and the N-terminal b-hairpin have been proposed to be the structural determinants responsible for ... C57–C108. The long-range NOEs between residues surrounding the cysteines confirm the cysteine pairing defined here. This pattern agrees with the arrangement present in other ribotoxins, and...
Ngày tải lên: 18/02/2014, 08:20