Báo cáo khoa học: Redox-sensitive loops D and E regulate NADP(H) binding in domain III and domain I–domain III interactions in proton-translocating Escherichia coli transhydrogenase potx

Tài liệu Báo cáo khoa học: Proteolysis of Pseudomonas exotoxin A within hepatic endosomes by cathepsins B and D produces fragments displaying in vitro ADP-ribosylating and apoptotic effects doc

... cleavages generated in vitro occurring mainly within domain III ofETA-A. Cell-free endosomes preloaded in vivo with ETA intraluminallyprocessed and extraluminally released intact ETA and ETA-A in vitro ... cathepsins B and D degraded ETA in apH-dependent manner, with maximal degradationbeing observed at pH 4. The ETA fragments generatedby the pure cathepsins (especially cathepsin B at pH 4)had ... the endosomal proteolysis of internalizedETA under conditions that maintained endosomeintegrity, we used cell-free endosomes containing in vivo internalized ETA (Fig. 3A,B). Endosomes wereisolated...

Báo cáo khoa học: Structure–function relationship of novel X4 HIV-1 entry inhibitors – L- and D-arginine peptide-aminoglycoside conjugates pptx

... that Neam-r9 and Neo-r9 may interfere with the binding of the viralenvelope to the cell. D- arginine (6- and 9-mers) peptide-aminoglycosides are readily internalized and concentrated in the cell ... to the desired compound. Solvents wereremoved under reduced pressure, and extracted three timeswith warm ethyl acetate. The ethyl acetate layer waswashed twice with water, dried over sodium ... byMALDI-TOF.Peptide synthesisArginine peptides l-, d- and l ⁄ d (6- and 9-mers), and theirN-terminal acetylated derivatives were synthesized manuallyby standard solid phase peptide synthesis technique...

Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redeﬁnition of the I-conotoxin superfamily pot

... electrodes were located in wells 1 and 2, and aground electrode in well 2. Recording electrodes werelocated in wells 3 and 4. Peptide was added by exchange ofthe ﬂuid contents of well 4. To eliminate ... d- phenylalanine residue in r11a is underlined).There is a notable difference in the sequence diver-gence from r11a at the C-terminal 12 amino acids(boxed residues in Table 1): the sequences of r11a and r11b ... stimulatingone end of the muscle every 30 s while recording from the other end, as described in Experimental procedures. Recordings are presentedas in Fig. 4. Thus, the lowest trace in each panel...

Báo cáo khoa học: Catalytic activation of human glucokinase by substrate binding – residue contacts involved in the binding of D-glucose to the super-open form and conformational transitions ppt

... ligand is engulfed in the cleft and held in place by extensive hydrogen-bonding inter-actions with residues in the L -domain (residues N204, D2 05, N231, E2 56 and E2 90) and the S -domain (resi-dues ... studies by demonstrating that the binding of Glc atthe active site indeed induces a large-scale domain move-ment that closes the active site cleft and creates thestereochemical environment ... was hindered by the low signal amplitude [24].Therefore, they also studied the equilibrium binding ofPNP-AMP to the enzyme, and reported a relativelylarge decrease in ﬂuorescence (DFeq⁄...

Báo cáo khoa học: "Effect of intern’s consecutive work hours on safety, medical education and professionalism"

... isfurther confirmed by the fact that there were no moremedications ordered or diagnostic tests interpreted in thetraditional schedule per patient-day, and there were in factfewer procedures performed ... other studies have suggested [8,9].Despite these relatively minor limitations, these two studiesoffer the best evidence to date that sleepy interns providebad patient care, and we applaud the ... differences theyobserved, because these data were presented as rates(errors/1000 patient-days). That the rates of medicationorders and test interpretations did not differ between groupscertainly...

Tài liệu Báo cáo khoa học: P25a ⁄ TPPP expression increases plasma membrane presentation of the dopamine transporter and enhances cellular sensitivity to dopamine toxicity pptx

... and p25aDC were incubated with the homogenate and treated like B-p25a, except they were detected with using p25a-1 antibody. Representative data from one of three independent experimentsare ... cloned into pET1 1d vector, expressed in E. coli and puriﬁed. The puriﬁed proteins were subjected toreducing SDS ⁄ PAGE and Coomassie blue staining. Molecular size markers are shown on the left. ... folded core domain possesses thestructure necessary for the vesicle binding function.However, direct evidence could not be obtained becausethe recombinant p25a core protein was insoluble at...

Tài liệu Báo cáo khoa học: Diol dehydratase-reactivating factor is a reactivase – evidence for multiple turnovers and subunit swapping with diol dehydratase pdf

... theenzyme and the reactivase were markedly reduced in density, and two bands of the enzymeÆreactivasecomplexes (upper and lower) appeared above them(Fig. 5A, lane c). When apoenzyme was used ... the reactivase eluted separately at theirrespective retention times. In contrast, a peak of the freereactivase decreased and a new peak appeared in thepresence of ADP. The latter peak was eluted ... the reactivase. One reason might be itslarger size, and the other possible reason might be thatthe additional interaction between its adenine moiety and the enzymes’s adenine -binding pocket stabilizesthe...

Tài liệu Báo cáo khoa học: MicroRNA-23a promotes the growth of gastric adenocarcinoma cell line MGC803 and downregulates interleukin-6 receptor pdf

... the predictedtarget genes are in large quantity and most have notbeen validated experimentally. Therefore, we used acDNA microarray to search for downregulated genes in gastric adenocarcinoma ... tissue samples. The genes thatwere predicted by two of the algorithm programs(pictar and targetscan release 5.1) and were alsodownregulated in our cDNA microarray were selectedas candidate targets ... the understanding and written consent of each subject.The study methodologies conformed to the standards set bythe Declaration of Helsinki, and were approved by the localethics committee.L...

Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... measured. For each assay, at least threeindependent yeast colonies were used.Direct interaction between APUM and RNA in theyeast three-hybrid systemTo investigate direct interaction of the ... withKlenow fragment of Escherichia coli DNA polymerase.After extension, the double-stranded DNA were digestedwith XbaI and SmaI and ligated to an AvrII-SmaI-digestedpRH5¢ vector. The sequences ... These four transcriptshave been described to code for proteins involved in diverse developmental processes, including shoot meri-stem organization, stem cell maintenance and mainte-nance of...

Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx

... similar central structuredregion connected by loops of different length. Indeed,extended loops and the N-terminal b-hairpin have beenproposed to be the structural determinants responsiblefor ... C57–C108. Thelong-range NOEs between residues surrounding thecysteines conﬁrm the cysteine pairing deﬁned here.This pattern agrees with the arrangement present in other ribotoxins, and is fully ... acid and a base on each side of the hydrolyzed bond [16]. E9 6 and H137 in a-sarcin and E5 8 and H92 in RNaseT1 act as the base and acid groups, respectively. Com-paring the 3D structures, E6 6 and...

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