Báo cáo khoa học: Directed evolution of a glutaryl acylase into an adipyl acylase potx

... a- aminoadipyl side chains varies greatly. As the glutaryl, adipyl and a- aminoadipyl side chains are all very similar, it can be envisaged that a glutaryl acylase is a good starting point for directed ... The enzyme deacylated glutaryl- 7-ACA with a catalytic con- stant k cat of 8.1 s )1 and with a Michaelis constant K m of 0.08 m M . Adipyl- 7-ADCA is deacylated at...

Ngày tải lên: 31/03/2014, 09:20

... his- tone acetyltransferase domain of the human PCAF transcriptional regulator bound to coenzyme A. EMBO J 18, 3521–3532. 35 Ahmad S, Gromiha MM, Fawareh H & Sarai A (2004) ASAView: solvent accessibility ... substrate specific- ity. P ⁄ CAF, p300 ⁄ CBP-associating factor, is a trans criptional coactivator with a variable N-terminal, a central HAT domain and a C-terminal bromod...

Ngày tải lên: 23/03/2014, 06:20

... compounds [12,13], and numerous studies of DHFR from many organisms [11]. The breadth of information available on DHFR and the significance of it in the treatment of a range of conditions makes it an ideal subject ... selection of drug targets. Along with a clear indication of the ease with which antibiotic resistance can develop and the means by which it can do so, direct...

Ngày tải lên: 19/02/2014, 02:20

... 4.1 aaa fi aga Variants of second generation E53V ⁄ K56R ⁄ C23S gaa fi gta 120% a 1600 42 aaa fi aga tgt fi agt a Residual activity was measured in 15% AA ⁄ 0.1% TEMED. Stabilisation of FDH for entrapment ... step of evolution. Stability of mutant FDHs in PAA Finally, the performance of the FDH variants after entrapment in PAA was investigated by measuring the activity in comparis...

Ngày tải lên: 16/03/2014, 13:20

... hydrolysis of the critical b-lactam ring and render the antibiotic inactive against its original cellular target, the cell wall transpeptidase. b-Lactamases of Keywords class C b-lactamase; cold adaptation; psychrophile; ... Lie ` ge-Sart, Tilman, Belgium b-Lactamases are the major causes of bacterial resis- tance to the b-lactam family of antibiotics, such as penicillins and cephalos...

Ngày tải lên: 23/03/2014, 07:20

... mutations Mousumi Banerjee 1 , Hemalatha Balaram 2 and Padmanabhan Balaram 1 1 Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India 2 Molecular Biology and Genetics Unit, Jawaharlal ... in enzymes: a study of triosephosphate isomerase and comparison with methyl glyoxalsynthase. Adv Protein Chem 66, 315–372. 45 Gunasekaran K, Ramakrishnan C & Balaram P (1996) Disa...

Ngày tải lên: 18/02/2014, 11:20

... by invertebrates, and has three crystal phases: calcite, ara- gonite and vaterite. Although calcite is the most stable crystal thermodynamically, many organisms can form metastable aragonite crystals ... region was decreased and a new band was detected at 64 kDa, but only after treatment with heparitinase II (Fig. 5A) . Although the same band was obtained after digestion Fig. 3. 45 Ca overl...

Ngày tải lên: 18/02/2014, 17:20

... absorption bands. Heme catabolism by HOs of mammals, pathogenic bacteria, cyanobacteria and probably insects is considered to have a similar mechanism, because the characteristic absorption bands of verdoheme ... higher plants have been identified in a few moss plants, several angiosperms (tobacco, tomato, pea, soybean, rice plant, sorghum, Arabidopsis thaliana), and a gym- nosperm (...

Ngày tải lên: 19/02/2014, 05:20

... smaller compound PEA (Fig. 1B) and a water molecule are present and PEX and PEA refined satisfactorily with occupancies of 0.7 Table 1. Data and refinement statistics. Values in parentheses pertain ... 25% of the accessible surface area of a monomer contributes to Ll PDH dimer formation. Whereas LlPDH and OaPDH have a buried surface area of  5500 A ˚ 2 , TbPDH has a larger in...

Ngày tải lên: 19/02/2014, 05:20

... change of the Fe(II) spin state and no replacement of any of the two axial ligands of the iron, His18 or RNO, by an amino acid side-chain of the antibody protein. Binding site topology of antibody ... the S-oxidation of thioanisole associated H 2 O 2 as an oxidant with MP8 as a catalyst in the presence of tBuOH as an organic co-solvent (Table 2), the stereoselective S-...

Ngày tải lên: 19/02/2014, 12:20