Báo cáo khoa học: Purification and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx

... pi-class GST. Studies of aquatic species such as salmonids [26], Bufo bufo [27] and M. edulis [28], showed that their GSTs belong to the pi class. The GST isoforms 1-1 and 2-2 from R. decussatus ... deals with the purification and the partial char- acterization of glutathione S- transferase (GST) isoforms from the clam Ruditapes decussatus. For the fi...

Ngày tải lên: 31/03/2014, 09:20

... 1977 effect of the d-Phe on structure, was also studied. The unusual structure of conomarphin further demon- strates the high diversity of conotoxins. Results Purification and primary sequence of conomarphin As ... [24–27]. Most NOESY crosspeaks were assigned and inte- grated, with concomitant cycles of structure calcula- tions for evaluation of distance and angle con...

Ngày tải lên: 18/02/2014, 17:20

... possessing a higher amplitude change in the stopped-flow measure- ments compared to phase 1. Otherwise, the k cat values from the substrate conversion assays were likely to be in the range of the ... 11-deoxy- corticosterone (DOC) to the products B (cor- ticosterone) and 18OH-B using cortisol (F) as internal standard. (B) shows the conver- sion of the substrate 11-deoxy...

Ngày tải lên: 30/03/2014, 04:20

... the bacterial species producing insecticidal toxins isolated from the larvae of M. bore is a strain of B. cereus. The B. cereus strain has been isolated repeatedly from the digestive system of ... 603 intensity data were fitted using PRISM software (Graphpad software Inc., San Diego, 5 CA, USA). Results and discussion The SCD culture broth of a Gram-positive bacterial s...

Ngày tải lên: 30/03/2014, 13:20

... the cDNAsindicateprimersusedforthePCR.LengthsofthecDNAsare shown in the parentheses. (B) Structure of the genomic fragment, Hdcel-1 DNA. Open and closed boxes represent exons and introns, respectively. The sequence ... region of the HdEG66 was amplified. The Hdcel-1 DNA consisted of four exons and three introns (Fig. 4B) and the coding sequence of the exons was...

Ngày tải lên: 20/02/2014, 23:20

... diagnosis and therapy, and an understand- ing of the structure of these allergens is essential to the improvement of diagnosis and the design of adequate therapeutic treatment. We previously identified ... sequences. Identical amino acids are shown as dots. The highly conserved and consensus amino acid residues involved in six Cys residues are indicated by aster- isks....

Ngày tải lên: 07/03/2014, 12:20

... further the structure of human PKI and understand the nature of the protein, here we report the purification and structural characteristics of human PKIb. Experimental procedures Materials Unless ... 1636 and 1625 cm )1 can be assigned to extended chain structures; the bands at 1683, 1669 and 1663 cm )1 are possibly assigned to turns or bends; and the band at 1647...

Ngày tải lên: 07/03/2014, 15:20

... help us to address this issue. The presence of two plastidic isoforms with different kinetic behavior raised the question of their respective physiological roles. The presence of several plastidic isoforms ... domains. The aim of the present study was to conduct a biochemical compar- ison of these two isoforms of arogenate dehydro- genase and a detailed kinetic a...

Ngày tải lên: 17/03/2014, 11:20

... concentrations, which becomes positive as the substrate levels are increased (data not shown). Similar results were obtained by Hill analysis. DISCUSSION The results describe the first purification of human ... loss of activity. At 4 °C the enzyme is stable for up to 2 weeks in the presence of PLP, and this is how crude enzyme solution was stored between successive steps. Ac...

Ngày tải lên: 08/03/2014, 22:20

... compositions of various cp-19ks and their deviations from standard compositions. The amino acid compositions of mature cp-19ks are presented as the number of residues per protein in columns 1–4. The ... surfaces versus time from the surface plasmon resonance (SPR) mea- surement. The proteins showed rapid adsorption to the sensor surfaces that corresponded to sharp incr...

Ngày tải lên: 16/03/2014, 05:20