Báo cáo khoa học: Puriﬁcation and partial characterization of seven glutathione S -transferase isoforms from the clam Ruditapes decussatus pptx

... pi-class GST.Studies of aquatic species such as salmonids [26], Bufobufo [27] and M. edulis [28], showed that their GSTsbelong to the pi class. The GST isoforms 1-1 and 2-2 from R. decussatus ... deals with the puriﬁcation and the partial char-acterization of glutathione S- transferase (GST) isoforms from the clam Ruditapes decussatus. For the ﬁrst step of puriﬁcation, two aﬃnity columns, ... characteristics of R. decussatus GST isoforms. Results are expressed as means ± SD. Reverse phase HPLC retention time(Rt) of subunits expressed in minutes; apparent molecular mass of each subunit...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... 1977effect of the d-Phe on structure, was also studied. The unusual structure of conomarphin further demon-strates the high diversity of conotoxins.Results Puriﬁcation and primary sequence of conomarphinAs ... [24–27].Most NOESY crosspeaks were assigned and inte-grated, with concomitant cycles of structure calcula-tions for evaluation of distance and angle constraintviolations as well as assignments of ... wereperformed using the parm03 force ﬁeld and the GB ⁄ SAimplicit solvation system [48]. The visual analysis of cono-marphin was done using molmol [49] software, and the geometric qualities of the obtained...

Báo cáo khoa học: Puriﬁcation and functional characterization of human 11b hydroxylase expressed in Escherichia coli doc

... possessing ahigher amplitude change in the stopped-ﬂow measure-ments compared to phase 1. Otherwise, the kcatvalues from the substrate conversion assays were likely to bein the range of the ... 11-deoxy-corticosterone (DOC) to the products B (cor-ticosterone) and 18OH-B using cortisol (F)as internal standard. (B) shows the conver-sion of the substrate 11-deoxycortisol (RSS)to F using 11-deoxycorticosterone ... velocity and the small amplitude change, it was not possible todetermine the Adx dependency of this ﬁrst process.Both the second phase and the third phase displayedan Adx dependency of the kobsrate,...

Báo cáo khóa học: Puriﬁcation and functional characterization of insecticidal sphingomyelinase C produced by Bacillus cereus ppt

... the bacterialspecies producing insecticidal toxins isolated from the larvae of M. bore is a strain of B. cereus. The B. cereus strain has been isolated repeatedly from the digestive system of ... 603intensity data were ﬁtted usingPRISMsoftware (Graphpadsoftware Inc., San Diego,5CA, USA).Results and discussion The SCD culture broth of a Gram-positive bacterial strainisolated from the ... also the insecticidal activity of the recombinant SMC was markedly reduced, suggesting aclose link between the insecticidal and enzyme activities.Materials and methodsInsectsLast instars of...

Tài liệu Báo cáo khoa học: Puriﬁcation and cDNA cloning of a cellulase from abalone Haliotis discus hannai ppt

... the cDNAsindicateprimersusedforthePCR.LengthsofthecDNAsareshown in the parentheses. (B) Structure of the genomic fragment,Hdcel-1 DNA. Open and closed boxes represent exons and introns,respectively. The sequence ... region of the HdEG66 was ampliﬁed. The Hdcel-1 DNA consisted of four exons and three introns(Fig. 4B) and the coding sequence of the exons wasconsistent with that of cDNA. This strongly suggests ... the mussel cellulase was also investigated by genomic PCRsimilar to the case of arthropod cellulases. According to the primary structure analysis, the mussel cellulase is classiﬁedinto the GHF45...

Báo cáo khoa học: Puriﬁcation and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot

... diagnosis and therapy, and an understand-ing of the structure of these allergens is essential to the improvement of diagnosis and the design of adequatetherapeutic treatment. We previously identiﬁed ... sequences. Identicalamino acids are shown as dots. The highly conserved and consensus amino acid residues involved in six Cys residues are indicated by aster-isks. The glycosylation site is ... Biosystems,Foster City, CA, USA). MALDI mass-spectroscopic analy-sis was performed on a Voyager DE-STR mass spectro-meter (PerSeptive Biosystems, Framingham, MA, USA).PCR-based cloning strategy...

Báo cáo khoa học: Puriﬁcation and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf

... further the structure of human PKI and understand the nature of the protein, here we report the puriﬁcation and structural characteristics of human PKIb.Experimental proceduresMaterialsUnless ... 1636 and 1625 cm)1can be assigned to extendedchain structures; the bands at 1683, 1669 and 1663 cm)1arepossibly assigned to turns or bends; and the band at1647 cm)1is likely to be the random ... [3,6]. Based onthese elements of sequence homology, the new protein isdesignated as human PKIb. Puriﬁcation and characteristics of recombinanthuman PKIbProtein expression and puriﬁcation was applied...

Báo cáo khoa học: Puriﬁcation and kinetic analysis of the two recombinant arogenate dehydrogenase isoforms of Arabidopsis thaliana pptx

... help us to addressthis issue. The presence of two plastidic isoforms with differentkinetic behavior raised the question of their respectivephysiological roles. The presence of several plastidic isoforms ... domains. The aim of the present study was to conduct a biochemical compar-ison of these two isoforms of arogenate dehydro-genase and a detailed kinetic analysis of their substratespeciﬁcity and ... than for their arogenate substrate. Thisstrong feedback inhibition arises from the necessity of the plant cell, to regulate the ﬂux of arogenate between the synthesis of tyrosine and that of phenylalanine....

Báo cáo Y học: Puriﬁcation and biochemical characterization of some of the properties of recombinant human kynureninase pptx

... concentrations, whichbecomes positive as the substrate levels are increased (datanot shown). Similar results were obtained by Hill analysis.DISCUSSION The results describe the ﬁrst puriﬁcation of human ... loss of activity. At4 °C the enzyme is stable for up to 2 weeks in the presence of PLP, and this is how crude enzyme solution was storedbetween successive steps.Activity assaysKynureninase ... metabolism.Rapid progress in the pathophysiology of human diseaseshas always been hampered by the availability of humantissue, aesthetics, and ethical considerations. The principleaim of this study...

Báo cáo khoa học: Identiﬁcation and functional characterization of a novel barnacle cement protein pptx

... compositions of various cp-19ks and their deviations from standard compositions. The amino acid compositions of mature cp-19ks are presented as the number of residues per protein in columns 1–4. The ... surfaces versustime from the surface plasmon resonance (SPR) mea-surement. The proteins showed rapid adsorption to the sensor surfaces that corresponded to sharp increases in the SPR shift. ... various advantages fororganisms; such organisms therefore have developed avariety of molecular systems to hold themselves on the boundary during their evolution. Marine sessile organ-isms possess...

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