Báo cáo khoa học: Xanthosine and xanthine Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems docx
Báo cáo khoa học: Xanthosine and xanthine Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems docx
... Xanth(os)ine and purine nucleoside phosphorylases (Eur. J. Biochem. 269) 4057 Xanthosine and xanthine Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems Gerasim ... aqueous medium [26]. Hitherto, thioxanthines and their nucleosides have not been examined as potential substrates of PNP, and studies of their...
Ngày tải lên: 31/03/2014, 09:20
Báo cáo khoa học: tmRNA from Thermus thermophilus Interaction with alanyl-tRNA synthetase and elongation factor Tu pptx
... interaction with similarstructural patterns (the alanylated acceptor stem with the specifically recognizable GU base pair, and the T loop). On the other hand, in an established complex with the enzyme, ... interesting to compare k f and k rev values, which characterize the enzyme binding to uncharged and alanyl- ated RNA, respectively. At the given conditions, both Ala$tmR...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... 2003 seen to interact with the N-terminal autoregulatory sequence (Fig. 4D and Table 1). The dopamine nitrogen interacts with the carbonyl oxygen of Ser23 and the dopamine Ca atom interacts with the ... cofactor onto the structure of the ligand- free rPAH (containing the regulatory and catalytic domains) [19] revealed that both the reduced and the oxidized cofactor interact w...
Ngày tải lên: 17/03/2014, 09:20
Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx
... 3A), causing the sulfur atom to point into the enzyme interior. The ribose O2¢ atom of exo-2¢,3¢-GCPT forms a hydrogen bond with the Glu56 OE1, and O3¢ forms a hydrogen bond with the side chain of ... hydrogen-bonding network with the enzyme, and this seems to be very important for proper enzyme base binding. Other bases form a lower number of hydrogen bonds, up to two,...
Ngày tải lên: 18/02/2014, 11:20
Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot
... site-directed mutagenesis [10–12]. The lysine residue is thought to be protonated in the free enzyme and unprotonated in the enzyme substrate complex, where it receives a pro- ton from the 3-hydroxyl group of 6PG as ... required to donate a proton to the C3 car- bonyl group of the keto-intermediate to facilitate decarboxylation. Both a base and an acid are needed in the tautom...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis pdf
... b strand 4 (catalytic proton donor) and the o ther after the b strand 7 (catalytic nucleophile). Family 1 comprises enzymes with 14 d ifferent EC numbers 3 , catalyzing hydro- lysis of substrates ... hydroxyls 4 and 6 were designated 4 and 6, where ÔeÕ stands for an equatorial hydroxyl and ÔaÕ for a n axial one. Therefore, the interaction between any residue at position 39 and an...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf
... this molecule. Substrate binding The substrate dT binds to the enzyme in a similar way to the dT moiety of the feedback inhibitor dTTP (Figs 1B and 2) and has the same interactions with the main ... results with hTK1 showed a pattern, with MeT, FIAU and AZMT form- ing a group of quite poor inhibitors with IC 50 values of 60–100 lm. The other analogues tested with h...
Ngày tải lên: 30/03/2014, 11:20
Báo cáo khoa học: Molecular basis for substrate recognition and drug ˚ resistance from 1.1 to 1.6 A resolution crystal structures of HIV-1 protease mutants with substrate analogs pptx
... the p1-p6 substrate [21]. PR interactions with substrate analogs compared to those with clinical inhibitors Substrate analogs showed more flexibility than clinical inhibitors in binding to the mutant ... extended interactions within S4 to S4¢. UIC-94017 is smaller than the substrate analogs but formed similar hydro- gen bonds to PR main chain atoms. Compared with indinavi...
Ngày tải lên: 30/03/2014, 20:20
Báo cáo khoa học: The heterogeneity of mast cell tryptase from human lung and skin Differences in size, charge and substrate affinity ppt
... lung and skin Differences in size, charge and substrate affinity Qi Peng 1 , Alan R. McEuen 1 , R. Christopher Benyon 2 and Andrew F. Walls 1 1 Immunopharmacology Group and 2 Tissue Remodelling and ... cell origin with trypsin-like substrate specificity [1,2]. Upon activation of these cells with allergen or other stimuli, it is released along with other potent mediators...
Ngày tải lên: 31/03/2014, 07:20
Báo cáo khoa học: " Medication errors: a prospective cohort study of hand-written and computerised physician order entry in the intensive care unit"
... component but without decision support. Prior to this, hand-written drug charts were used. With both prescribing systems, prescribing was restricted to intensive care medical staff only. To compare both ... and the ICU clinical director, according to an adapted scale [9-11]. Minor errors were classified as those causing no harm or an increase in patient monitoring with no chang...
Ngày tải lên: 25/10/2012, 10:39