Báo cáo khoa học: Xanthosine and xanthine Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems docx
Báo cáo khoa học: Xanthosine and xanthine Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems docx
... Xanth(os)ine and purine nucleoside phosphorylases (Eur. J. Biochem. 269) 4057 Xanthosine and xanthine Substrate properties with purine nucleoside phosphorylases, and relevance to other enzyme systems Gerasim ... aqueousmedium [26].Hitherto, thioxanthines and their nucleosides have notbeen examined as potential substrates of PNP, and studiesof their substrates’ properties with other enzymes have notconsidered ... erythrocytes and calf spleen with both Xao and Xanis in the range 5–6, whereas those with Guo and Gua, and Ino and Hx, are in the range 7–8. The pH-dependence of substrate properties of Xao and Xan...
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Báo cáo khoa học: tmRNA from Thermus thermophilus Interaction with alanyl-tRNA synthetase and elongation factor Tu pptx
... interaction with similarstructural patterns (the alanylatedacceptor stem with the specifically recognizable GU basepair, and the T loop). On the other hand, in an establishedcomplex with the enzyme, ... interesting to compare kf and krevvalues, whichcharacterize the enzyme binding to uncharged and alanyl-ated RNA, respectively. At the given conditions, bothAla$tmRNA and Ala$tRNAAlaassociate with ... tmRNAcharging with alanyl-tRNA synthetase in vitro in theabsence of other proteins was found to be slow and inefficient in comparison with tRNA alanylation. SmpBimproves significantly the substrate properties...
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Báo cáo khoa học: Studies on the regulatory properties of the pterin cofactor and dopamine bound at the active site of human phenylalanine hydroxylase pptx
... 2003seen to interact with the N-terminal autoregulatorysequence (Fig. 4D and Table 1). The dopamine nitrogeninteracts with the carbonyl oxygen of Ser23 and thedopamine Ca atom interacts with the ... cofactor onto the structure of the ligand-free rPAH (containing the regulatory and catalytic domains)[19] revealed that both the reduced and the oxidized cofactorinteract with the N-terminal autoregulatory ... dihydroxypropyl side-chain and Ser23O inthe autoregulatory sequence (Fig. 4C and Table 1) and thusexplaining the complete reversal of the pterin cofactor as anegative effector (Figs 1 and 3A,B).When...
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Tài liệu Báo cáo khoa học: Structure of RNase Sa2 complexes with mononucleotides – new aspects of catalytic reaction and substrate recognition pptx
... 3A), causing the sulfur atom to point into the enzyme interior. The ribose O2¢ atomof exo-2¢,3¢-GCPT forms a hydrogen bond with theGlu56 OE1, and O3¢ forms a hydrogen bond with theside chain of ... hydrogen-bonding network with the enzyme, and this seems to be very important forproper enzyme base binding. Other bases form a lowernumber of hydrogen bonds, up to two, and have worsefits in ... base-binding site appears to be too large;for cytosine and uracil, we observed both horizontalshifts and rotation of the base with respect to theplane of the guanine, by up to 40°, disrupting...
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Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot
... site-directedmutagenesis [10–12]. The lysine residue is thought to be protonated in the free enzyme and unprotonated inthe enzyme substrate complex, where it receives a pro-ton from the 3-hydroxyl group of 6PG as ... required to donate a proton to the C3 car-bonyl group of the keto-intermediate to facilitatedecarboxylation. Both a base and an acid are neededin the tautomerization of the enediol intermediate to yield ... thanthe substrate per se. To better understand why these analogues have highaffinity and to help in rational drug design, we under-took a thermodynamic characterization of substrate and analogue...
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Báo cáo khoa học: Investigation of the substrate specificity of a b-glycosidase from Spodoptera frugiperda using site-directed mutagenesis and bioenergetics analysis pdf
... b strand 4 (catalytic proton donor) and the o ther afterthe b strand 7 (catalytic nucleophile). Family 1 comprisesenzymes with 14 d ifferent EC numbers3, catalyzing hydro-lysis of substrates ... hydroxyls 4 and 6 were designated 4 and 6, where ÔeÕ stands for an equatorialhydroxyl and ÔaÕ for a n axial one. Therefore, the interactionbetween any residue at position 39 and an equatorial 4-OHwas ... steps (DDGà), corresponding to a pair ofdifferent substrates hydrolyzed by the same enzyme, or to a pair of different enzymes hydrolyzing the same substrate (Figs 2 and 3), were calculated by...
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Báo cáo khoa học: Structure of the substrate complex of thymidine kinase from Ureaplasma urealyticum and investigations of possible drug targets for the enzyme pdf
... this molecule. Substrate bindingThe substrate dT binds to the enzyme in a similar way to the dT moiety of the feedback inhibitor dTTP(Figs 1B and 2) and has the same interactions with themain ... results with hTK1showed a pattern, with MeT, FIAU and AZMT form-ing a group of quite poor inhibitors with IC50valuesof 60–100 lm. The other analogues tested with hTK1fell into a group with ... hTK1, with specificity for pyrimidine deoxynucleosides and with dTTP serving as a feedback inhibitor [11,13]. Uu-TK isless fastidious than hTK1 with regard to phosphatedonors, using all nucleoside...
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Báo cáo khoa học: Molecular basis for substrate recognition and drug ˚ resistance from 1.1 to 1.6 A resolution crystal structures of HIV-1 protease mutants with substrate analogs pptx
... thep1-p6 substrate [21].PR interactions with substrate analogs compared to those with clinical inhibitors Substrate analogs showed more flexibility than clinicalinhibitors in binding to the mutant ... extendedinteractions within S4 to S4¢. UIC-94017 is smallerthan the substrate analogs but formed similar hydro-gen bonds to PR main chain atoms. Compared with indinavir and other clinical inhibitors, UIC-94017formed ... PRV82A and P1¢ and P1 of the substrate analog compared with those of PR (Fig. 6D). Theimproved interactions with p1-p6 were consistent with the threefold better inhibition of PRV82Athan PR, and with...
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Báo cáo khoa học: The heterogeneity of mast cell tryptase from human lung and skin Differences in size, charge and substrate affinity ppt
... lung and skinDifferences in size, charge and substrate affinityQi Peng1, Alan R. McEuen1, R. Christopher Benyon2 and Andrew F. Walls11Immunopharmacology Group and 2Tissue Remodelling and ... cellorigin with trypsin-like substrate specificity [1,2]. Uponactivation of these cells with allergen or other stimuli, it isreleased along with other potent mediators of inflammationincluding other ... protonated arginines, to interact with the heparin to delay inactivation of the enzyme, but bypH 10.5, too many of the lysine residues have becomedeprotonated for heparin to afford any stability.Although...
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Báo cáo khoa học: " Medication errors: a prospective cohort study of hand-written and computerised physician order entry in the intensive care unit"
... component butwithout decision support. Prior to this, hand-written drugcharts were used. With both prescribing systems, prescribingwas restricted to intensive care medical staff only. To compareboth ... and the ICU clinical director, according to anadapted scale [9-11]. Minor errors were classified as thosecausing no harm or an increase in patient monitoring with nochange in vital signs and ... have been to give 1 g and then to repeat when the plasma levels fell below 10 mg/L. Thedose as prescribed would have lead to nephrotoxicity.There were many cases of minor errors with CPOE that...
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Từ khóa: Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngchuyên đề điện xoay chiều theo dạngNghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THzNghiên cứu tổ chức chạy tàu hàng cố định theo thời gian trên đường sắt việt namBiện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển du lịch bền vững trên cơ sở bảo vệ môi trường tự nhiên vịnh hạ longĐịnh tội danh từ thực tiễn huyện Cần Giuộc, tỉnh Long An (Luận văn thạc sĩ)Thiết kế và chế tạo mô hình biến tần (inverter) cho máy điều hòa không khíSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXKiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)BT Tieng anh 6 UNIT 2Tăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Tranh tụng tại phiên tòa hình sự sơ thẩm theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn xét xử của các Tòa án quân sự Quân khu (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vậtchuong 1 tong quan quan tri rui roGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtGiáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vậtĐổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam