Báo cáo khoa học: ISC1-encoded inositol phosphosphingolipid phospholipase C is involved in Na+/Li+ halotolerance of Saccharomyces cerevisiae pptx

... ISC1-encoded inositol phosphosphingolipid phospholipase C is involved in Na + /Li + halotolerance of Saccharomyces cerevisiae Christian Betz 1 , Dirk Zajonc 1 , Matthias Moll 2 and Eckhart Schweizer 1 1 Lehrstuhl ... pathway being affected in the isc1D mutant. In agreement with this conclusion, cellular glycerol levels increased to comparable levels in wild-type...

Ngày tải lên: 31/03/2014, 09:20

... [22]. This mechanism is, however, hard to consider as the main underlying mechan- ism of LPS-induced IFN -c production in vivo,inviewofthe large amount of IFN -c (detectable as protein level) secreted in ... be effective for inducing the binding of IRF-1toregion2,leadingtoiNOS gene expression. The important role of IFN -c in the pathogenesis of LPS- induced shock was confi...

Ngày tải lên: 17/03/2014, 10:20

... Synoviolin C3 07A mutant was gener- ated by overlap PCR using the following primers: 5¢-AAATGTGGTTGGCGGGCAGTCTCTTGGC-3¢ and 5¢-ACTGCCCGCCAACCACATTTTCC-3¢. The PCR pro- duct was verified by sequencing. ... change in the intracellular trafficking of NCT. Effect of Synoviolin on the production of Ab NCT is one of the essential cofactors of the c- secretase complex. We therefore inv...

Ngày tải lên: 23/03/2014, 04:20

... collected by centrifuga- tion, and counted in a c- counter. Speci c [ 125 I]IGF-II bind- ing was determined by subtracting c. p.m. ligand bound in replicate reactions carried out in the presence ... receptor in clathrin- coated pits compared to microsomes [37,38]. During receptor-mediated endocytosis, when there is a high accumulation of receptors into one location, it is pos...

Ngày tải lên: 18/02/2014, 08:20

... was incubated for 24 h at 37 C with membranes isolated from colchicine treated pHb-1 cells or recombi- nant human MT1-MMP catalytic domain in the presence of increas- ing concentrations of TIMP-1 ... hard compositions of bone to the soft structures of connective tissue. Collagens are the main components of ECM, and type I collagen is the most abundant form in bone and connect...

Ngày tải lên: 18/02/2014, 11:20

... change in the isoelectric point, likely caused by the modification of a few amino acid residues upon binding of aminopentynal. This binding seems to be nonspeci c, as the existence of some micro- heterogeneity ... Science, Palacky ´ University, Olomouc, Czech Republic; 4 Department of Analytical Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic; 5 Department...

Ngày tải lên: 19/02/2014, 16:20

... efficiency of error correction process of this workbench. The re- sult shows that about 63.2 % of tagging errors can be corrected. 1 Introduction Natural language processing system using cor- ... 1993) is as follows: 1. Train initial tagger on initial training cor- pus Co. 2. Make Confusion matrix with the result of comparing the current training corpus Ci (initially, i 0)...

Ngày tải lên: 08/03/2014, 05:21

... shown, changed nucleo- tides are underlined. Variant Oligonucleotide sequence (5¢ to 3¢) L56A CCCGTTGGATCGGGT GCCTCGCCTAATGGGATTG L5 6C CCCGTTGGATCGGGT TGCTCGCCTAATGGGATTG L56F CCCGTTGGATCGGGT TTTTCGCCTAATGGGATTG L56H ... M (1997) Isolation of a cDNA coding for l-galactono -c- lactone dehydrogenase, an enzyme involved in the biosynthesis of ascorbic acid in plants. Purification, cha...

Ngày tải lên: 23/03/2014, 07:20

... 5¢-CACGCAAACAAGTGATTCAGC AGTGCG[FAM]G-3¢,5¢-CAGCCGAAACATCATCAGA GCAATGGC[FAM]G-3¢,5¢-CGGATTTCCGCTCACTCT TTATC[FAM]G-3¢ and 5¢-GACCTCCCATAACACCAG TATGTCGAGG[JOE ]C- 3¢ (Invitrogen Corporation, Carls- bad, ... 5¢-GACGACGACAAGATGGACGACGTCG TTGCACTCACAGAG-3¢ and 5¢-GAGGAGAAGCCCGGT TCAAGCAAAGATAGATAAGATGTTC-3¢ for GmPDIS-2, 5¢-GACGACGACAAGATGAAGGAGG AAGCCACCAA GTTGGGG-3¢ and 5¢-GAGGAGAAGCCCGGTCTAG...

Ngày tải lên: 23/03/2014, 09:21

... four binding motifs for [4Fe)4S] clusters. HdrC was shown to contain two binding motifs for [4Fe)4S] clusters while in subunit HdrB no characteristic binding motif of any known cofactor could ... of low molecular mass fractions of cell extracts to purified HmeCD or Mvh:Hdl in the presence of an oxidant. This could result in higher spin concentrations of the paramagnetic species...

Ngày tải lên: 30/03/2014, 13:20