... antimicrobial assayand in planta studies demonstrated that NtKTI1 is anantifungal protein that increases the resistance oftobacco to fungal attack.ResultsIsolation and characterization of a cDNA encod-ing ... Planta 183,528–535.27 Yamagata H, Kunimatsu K, Kamasaka H, KuramotoT & Iwasaki T (1998) Rice biofunctional alpha-amy-lase ⁄ subtilisin inhibitor: characterization, localization,and changes ... shown as a loading control.SQRT-PCR analysisTotal RNA was extracted from tobacco seedlings using the RNeasy Plant Mini kit and treated with RNase-freeDNase-I (Takara, Dalian, China) to remove...