... indicated clonal expansion of < /b> T-cells bearinghuman Vb3, Vb12, Vb17 and < /b> Vb19 [20]. Others showedproliferation of < /b> human T-cells bearing Vb1, V b2 , Vb3,Vb5.2, Vb12, Vb15 and < /b> Vb17 domains and < /b> found ... treatment of < /b> several pathologies and < /b> because of < /b> the< /b> potential use of < /b> the < /b> toxins as biological weapons. Alteration of < /b> their MHC and < /b> TCR binding capacity by site directedmutagenesis could be useful ... 5¢-CATGCCATGGCCAGTAGTCAGCCTGACCCTACTCCAG-3¢ and < /b> 3¢ primer, 5¢-GGTTATCATATAAAGATCTCAAATTACCC-3¢, for the < /b> second PCR the < /b> primers were: 5¢ primer, 5¢-GGGTAATTTGAGATCTTTATATGATAACC-3¢ and < /b> 3¢ primer, 5¢-CGCGCGGGATCCTTAGTGATGGTGATGGTGATGGGTGACCGGTTTTTTGGTAGGTGAAC-3¢.ThethirdPCRwascarried...