Báo cáo khoa học: Structural basis for poor uracil excision from hairpin DNA An NMR study pptx

... Structural basis for poor uracil excision from hairpin DNA An NMR study Mahua Ghosh 1 , Nidhi Rumpal 2 , Umesh Varshney 2 and Kandala V. R. Chary 1 1 Department ... K m are poor for both U1 and U3-hairpins as in the case of U2 -hairpin. On the other hand, as described earlier both the U 12 and U 14 bases in both U1- and U3-hairpins adopt an anti conformation ......

Ngày tải lên: 31/03/2014, 09:20

... Project on Protein Structural and Functional Analyses and a grant-in-aid for Scientific Research (16370048) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. This research ... cleft formed by the two domains. A common functional conformational change has been reported in this protein family, which involves switching from an open form to a sugar-transpo...

Ngày tải lên: 19/02/2014, 00:20

... [2-(2,4-dichlorophen- oxy)propanoic acid], with RdpA transforming the (R)-enantiomers and SdpA being specific for the (S)-enantiomers [33,34]. In addition, the nonheme oxygenase AspH from P. syringae hydroxylates ... than 2%. Research Collaboratory for Structural Bioinformatics protein data bank accession numbers Crystal structures and structure factors were deposited in the Research C...

Ngày tải lên: 16/03/2014, 02:20

... to thank Ms Barbara Smo ´ lska for her excellent technical assistance. We also thank the reviewers for their comments on the manuscript. This work was sup- ported by grant 6 P04A 081 21, from ... nucleotides shorter than in the case of aptamer no. 18). The determined j values were very similar: 4.0 and 3.6 for G27 and G28 in aptamer no. 18 and 3.7 and 3.5 for G25 and G26 in aptamer...

Ngày tải lên: 16/03/2014, 06:20

... original Slovakian government works Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase L ˇ ubica Urba ´ nikova ´ 1 ,Ma ´ ria Vrs ˇ anska ´ 2 , Kristian B. R. Mørkeberg ... Grange D, van Zyl WH & Prior BA (2000) A chromogenic substrate for a b-xylosidase- coupled assay of a-glucuronidase. Anal Biochem 286, 289–294. 29 Ryabova O, Vrs ˇ anska ´...

Ngày tải lên: 22/03/2014, 16:20

... closely related systems, PAL and con A, can be used as a basis for theoretical calculations and force-field valid- ation and may help towards correct prediction and a true understanding of biological ... carbohydrates and ovalbumin to concanavalin A. Biochemistry 33, 1149–1156. 31 Moothoo DN, Canan B, Field RA & Naismith JH (1999) Mana(1–2)Mana-OMe-concanavalin A complex reveals a bala...

Ngày tải lên: 23/03/2014, 11:20

... col- oured red for arginine, blue for glutamate and aspartate, green for histidine, cyan for polar, orange for aromatic and aliphatic side chains, brown for Thr77–Gly79 and dark red for Gly153-Asp176. ... stereospecificity at C4 of NAD(P) for alcohol dehydrogenase from Methanogenium organophilum and for (R)-2-hydroxyglutarate dehydrogenase from Acida- minococcus fermentan...

Ngày tải lên: 23/03/2014, 13:20

... BQU and CSD2006-00023. We would like to thank Fernando Roncal (CBM, Madrid) for synthesis of the pepscan cellulose membranes and Peter Rapali (Budapest, Hungary) for careful revision of the manuscript. References 1 ... and (K ⁄ R)(D ⁄ E)- TGIQVDR [11,14,15]. In both cases, the polypeptide stretches that associate with DYNLL1 adopt an extended conformation and form an additional b-stra...

Ngày tải lên: 29/03/2014, 09:20

... LD 100 for AZT was 300-fold for the double mutant N45D ⁄ N64D and mutant N64D, but only threefold for mutant N45D. Because in human cells AZT is mainly a substrate for TK1 (human TK1; hTK1) we also ... k cat ⁄ K 0.5 (nucleoside analog)] [22]. For the mutants N45D and N64D and the double mutant N45D ⁄ N64D this equation predicts an increase in catalytic efficiency for the phosphor...

Ngày tải lên: 30/03/2014, 20:20

... mapped onto the LRR and AXH domain of Anp32 and Atx1 respectively, and was shown to be stronger for expanded Atx1 [16,18]. The temporal and cell-specific expression pattern of Anp32 in cerebellar ... here a study of the structural determinants for the interactions of Anp32a with other partners. As a prerequisite for binding stud- ies, we first solved the structure in solution of Anp32...

Ngày tải lên: 18/02/2014, 17:20