Báo cáo khoa học: Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2) potx

... cture of n NOS-syntrophin complex. Science 28 4,8 12 815.1876 A. T. Nies et al. (Eur. J. Biochem. 26 9) Ó FEBS 20 02 Structural requirements for the apical sorting of human multidrug resistance protein ... part of MRP2 containsinformation for apical sorting of MRP2. Apical localization of GFP–MRP2 in polarized HepG2and MDCKII cellsA s equence alignment of the C-terminal ends of human MRP1, MRP2, ... PCR-fragment that enabledÓ FEBS 20 02 Apical sorting of human MRP2 (Eur. J. Biochem. 26 9) 1867 the in-frame insertion of GFP a t the N-terminus of MRP2(pMRP2.1). The sense primer for this PCR reaction...

Báo cáo khoa học: Structural basis for the erythro-stereospeciﬁcity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

... 6 421 28 22 0 12 Tel: +49 0 6 421 28 22 0 32 E-mail: essen@chemie.uni-marburg.de;marahiel@staff.uni-marburg.de(Received 31 March 20 09, revised 30 April 20 09, accepted 5 May 20 09)doi:10.1111/j.17 42- 4658 .20 09.07085.x The ... deposited in the Research Collaboratory for Structural Bioinformaticsunder accession numbers 2WBO for the substrate•tar-trate•iron complex, 2WBQ for the complex with hArg, and2WBP for the complex ... 0.063 (0 .24 9)Wilson B-factor (A˚ 2 ) 12. 4 9.07 9. 62 ReﬁnementRworka0.161 (0.305) 0.143 (0 .29 3) 0.1 32 (0 .25 2)Rfreea0 .21 2 (0.304) 0.178 (0.336) 0.171 (0 .29 3)Used reﬂections 74 865 121 303...

Báo cáo khoa học: Structural requirements for antimicrobial versus chemoattractant activities for dermaseptin S9 pptx

... Universidad 20 01, Col Chamilpa, Cuernavaca, Mor. 622 70, MexicoFax: + 52 731 723 88Tel: + 52 5 55 66 22 76 63E-mail: yvonne@ibt.unam.mx(Received 29 April 20 08, revised 11 June 20 08, accepted 16 June 20 08)doi:10.1111/j.17 42- 4658 .20 08.06554.xDermaseptin ... asurface area of approximately 5 nm 2 ; in the b-structureformation, the peptide has a contact area of 10– 12 nm 2 [25 ]. Consequently, the hydrophobic surfacecould bind 0.9 ngÆmm )2 of helical ... Further-0 5 10 150 20 406080100D 2 OPBSNH content (%)Time (min)0 50 100 150 20 0 25 0 3000 25 05007501000 125 015001750Resonance units (RU)PS9 concentration (µM) 20 0 22 0 24 0–40 20 0 20 4060PBSPBH 2 OΔε...

Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt

... 2. 85 ⁄ 2. 78 2. 85 ⁄ 2. 75 2. 78 ⁄ 2. 77 2. 75 ⁄ 2. 84Gly106(N) Man(O3) 2. 86 ⁄ 2. 94 2. 97 ⁄ 2. 99 2. 83 ⁄ 2. 80 2. 96 ⁄ 2. 84Asn138(ND2) Man(O4) 3.00 ⁄ 2. 92 2.93 ⁄ 2. 93 2. 95 ⁄ 2. 93 3.15 ⁄ 2. 98Glu 221 (N) ... statistics.Ligand-removed MannoseGlcNAc(b1 2) ManGlcNAc(b1 2) Man(a1–3)ManOMe M5 92 NA2FSpace group P21 2 1 2 1P21 2 1 2 1P21 2 1 2 1P21 2 1 2 1P21 2 1 2 1P21 2 1 2 1Beamline X11 X11 X11 X11 X11 ... the corresponding orientation of the side chain of Glu 221 . The second conformation of the side chain of Glu 221 , which also adopts twoconformations but not correlated with the anomeric form of...

Báo cáo khoa học: Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A docx

... side of the dimer surface, and extends the b-sheet dimer interface.Residues R 421 –C 427 of the GRINL1A peptide adopta b-strand conformation as assumed by residuesK 229 –V235 of nNOS peptide, and the ... area.Figure 4A shows the 20 lowest-energy conformersfrom the selected cluster modelled using the Haddockdocking calculations. The rmsd values for the 20 lowest-energy conformers of the best cluster ... interactionFEBS Journal 27 7 (20 10) 23 40 23 50 ª 20 10 The Authors Journal compilation ª 20 10 FEBS 23 43 The exchange regime for the residues mentionedabove is slow in the NMR time scale. Two reso-nances...

Báo cáo khoa học: Structural requirements for Caenorhabditis elegans DcpS substrates based on ﬂuorescence and HPLC enzyme kinetic studies pdf

... at the 2 or 3¢ oxygen of m7Guo The kinetic parameters obtained for m 2 7 ,2 -OGpppGand m 2 7,3¢-OGpppG demonstrated the crucial role of the 2 -OH and 3¢-OH groups of the m7Guo moiety for C. ... 0.18 3. 12 ± 1.45 5.09 ± 2. 80bn7GpppG bn7GMP + GDP 1.83 ± 0.15 3.06 ± 0. 12 1.67 ± 0.15m3 2, 2,7GpppG m3 2, 2,7GMP + GDP 3.85 ± 0.41 4.65 ± 0 .26 1 .21 ± 0.15m3 2, 2,7GpppA m3 2, 2,7GMP ... thatm3 2, 2,7GpppA and m 2 7,3¢-OGpppG compete for the same binding site in the inhibitory experiment [26 ]. The kinetic parameters obtained for m 2 7 ,2 -OGpppGand m 2 7,3¢-OGpppG indicate that the...

Báo cáo khoa học: Structural basis for the changed substrate speciﬁcity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx

... K0.5 of the mutants compared tokcat⁄ K0.5 of Dm-dNK (-fold)dTN45D 0.9 5.3 1 2. 6 2. 9 · 1064.1N64D 23 .2 1.7 0.8 0. 82 35344 473N45D ⁄ N64Da 24 .2 2.5 1 1 .22 50000 24 0Wildtypeb1 .2 29.5 ... 6N64D 3 820 0. 82 0.9 0.4 105 876N45D ⁄ N64Da3166 1.7 1 0. 828 26 0 354Wildtypeb 22 5 42. 7 1 20 .6 920 00 1dGN45D 4 12 2.7 1 1.3 3155 7.3N64D 20 350 0 .24 0.5 0. 12 5.9 3898N45D ⁄ N64Da 20 04 0.156 ... Inc.,USA. 29 Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein util-izing the principle of protein- dye binding. Anal Biochem 72, 24 8 25 4.30...

Báo cáo khoa học: Structural basis for stereo-speciﬁc catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans pptx

... NAD+-dependentD -2- hydroxyisocaproate dehydrogenase.FEBS Journal 27 2 (20 05) 26 9 28 1 ª 20 04 FEBS 26 9(Prelog–Helmchen Re ⁄ Si-system [5]), at the C4N atom of the nicotinamide moiety of the reduced ... aresolution of 1.98 A˚. The structural analysis of HGDHtogether with the comparison with other structurallycharacterized members of the D -2- hydroxyacid dehy-drogenase protein family further contributes ... bottom of the inter-domain cleft at approximately 12 A˚below the surface of the protein with the catalytic triad Arg235, Glu264and His297 facing into the cleft (Fig. 4, and Fig. 5A for a closer...

Báo cáo khoa học: "A Program for the Machine Translation of Natural Languages" pdf

... eliminate the cause of the incorrect translation. Before the account of the program itself we give a short sketch of the considerations which led to the program, to- gether with a statement of the ... letting the sequence translate as the sequence (in the same order) of the translations of the symbols. The fact that there are only finitely many symbols, together with the uniqueness of the repre- ... sequences of elements of U. In other words, U is the set of generators of the free semigroup that is the domain of T. Suppose that a and b are two of the units of U, and that T (a) = T(b) = If then,...

Báo cáo khoa học: "CONTEXT-FREENESS AND THE COMPUTER PROCESSING OF HUMAN LANGUAGES" pot

... interested in the computational properties of either kind of language, we will be interested in their language-theoretic properties, as well as properties of the grammars that define them and the parsers ... undeclared vari- ables make no sense either to the compiler or to the CPU. But they are still programs, provided they conform in all other ways to the syntax of the language in question, just as ... combinations of values for all other features). Because economical techniques for rapid checking of relevant feature values are built into the parsers normally used for such grammars, the size of the...

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