Báo cáo khoa học: The fate of newly synthesized V-ATPase accessory subunit Ac45 in the secretory pathway doc

Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... pro-tein–protein interaction. In this study, Osh6p wasdemonstrated to bind a pool of phosphatidylinositolphosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P,PtdIns(3,4)P2and PtdIns(3,5)P2with ... provided invaluable in- sights into understanding the function of this family of proteins in yeast and offered guidance to futureresearch. On the other hand, although the entire Oshprotein family ... Osh6p may bind phospholipidsthrough a domain other than PH. In support of this, the short Kes1p containing no PH domain was alsoshown to bind PtdIns (4,5)P [15]. Although the role of the conserved...

Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx

... data in the literature thatindicate PKCe as the only protein kinase isoform involved in the s ignalling pathway downstream of muscarinic m3receptors in SK-N-BE(C) neuroblasto ma cells [15]. The Ó ... PKCe in the r egulated processing of APP. Thereis substantial evidence in the literature for a signiﬁcant role of PKCe both in the regulation of APP metabolism [11–14]and in the pharmacology of ... regardless of a fullactivation of MAP-kinase demonstrates that the lattersignalling system is not involved in the carbachol-mediatedregulated processing of APP in these cells. In summary, the results...

Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx

... coupling of the two calcium binding sites within G2and G6. Additional calcium binding in other domains thenresult in the complete opening and activation of the gelsolin molecule.Keywords: actin; ... to G2 domain.Footprint of G2 on gelsolin G4–6 domainTwo sites within the G2 domain appear involved in the G2- G4–6 interface in the presence of EGTA (Fig. 3). The interaction of these two sites ... the C-terminal a-helix of G6 domain. The sequence 159–193, including the second actin interface,appears also in interaction with G6 domain. Therefore, wetested the interaction of G4–6 domain...

Báo cáo khoa học: Identiﬁcation of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx

... with the observed differences in the NOE contacts for the Glc I to Hep I linkage. In all threestrains examined, in which the Hep I residue of the LPSbears the additional Glc II residue at the ... [10]. The remainder of the anomeric resonances in the low-ﬁeld region (4.45–6.00 p.p.m.) of the spectrum were all attributable tob-linked residues by virtue of their chemical shifts and, in the ... conservedinner-core structure of meningococcal LPS has led to the utilization of mutants defective in the enzyme, resulting in truncation of the oligosaccharide chains of the LPS at the glucose residue at...

Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4NLS peptide. The NLS peptide binds to the major binding site in anextended ... cargo binding sites, referred to as the major and minor binding sites [1]. These sites arelocated in the concave face of the protein near regions of invariant Trp and Asn arrays. The major bindingsite ... signiﬁcant interactions with importin-a.This primarily occurs by the formation of a hydrogenbond between the side-chain hydroxyl group of Tyr205and the importin-a main chain at the C-terminus of the...

Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt

... two termini of the inserted Ca2+-binding sites in the model structure of the EF-hand of STIM1 is within15 A˚. Accordingly, a total of six glycine linkers is sufﬁ-cient to enable the grafted ... the initial mixture. The ﬂuorescence intensity wasnormalized by subtracting the contribution of the baselineslope using logarithmic ﬁtting. The Tb3+-binding afﬁnity of the protein was obtained ... Ca2+. According to calculationsusing the spherical shape of macromolecules, the hydrodynamic radius of the protein will increase by27% on formation of the dimer [22]. The increase in size for...

Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt

... structures of WNV NS2B–NS3pro havebeen reported in the absence of inhibitor [4] and in the presence of peptide inhibitors [5,6] or bovine pancre-atic trypsin inhibitor (BPTI) [4]. In the absence of inhibitor, ... may be because of spin-diffusion during the mix-ing time of the NOESY experiment, movements of the ligand in the binding pocket or differences in side-chain orientations between the crystal and ... observed in the crystal structures of the complex with peptidicinhibitors [4–6], in which the C-terminal b-hairpin of NS2B is positioned near the substrate-binding siterather than far away as in the...

Báo cáo khoa học: Combined use of selective inhibitors and ﬂuorogenic substrates to study the speciﬁcity of somatic wild-type angiotensin-converting enzyme docx

... composition in the binding site of thesethree ACE species were pinpointed. Together these data demonstrate that the substrate selectivity of the N-domain and C-domain depends on the ACE species. These ... by the N-domainand C-domain (indicated by the arrow in Fig. 1A).Thus, information on the selectivity of both the sub-strate and the inhibitor for the N-domain andC-domain can be determined ... RXP407. At theseconcentrations, RXP compounds mainly inhibit the activity of one active site, allowing the determination of the kineticparameters of the other active site, free of inhibitor....

Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the speciﬁcity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

... its S1¢ binding subsite. Substrate bindinginduces conformational changes involving the ArgA145-PheA146 fragment of the protein chain, acting as a ﬂap, ﬁrstopening and then closing the groove ... with the O atom of the main chain CO group of the TrpB240;Ng1shares H bonding with O atom of the COgroup of LeuB387 together with O atom of c-OH of SerB386; Ng2is in H bonding withOd1 of ... X-raycoordinates of amino acid residues in penicillinacylase [19] and AM1 docking calculations. The three N atoms of the side chain d-guanidinogroup of this residue are involved in H-bonding as...

Báo cáo khoa học: Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration ppt

... within the ﬁrst 16 aminoacids, as their removal results in loss of neuroprotec-tive phenotype [20,21]. Interestingly, the only knownbinding partner of the N-terminal region, AAAATPase valosin-containing ... The K14 Cre induces a full dele-tion when bred from the female as K14 is expressed in the oocyte [27]. The offspring of this cross had recom-bination between the two loxP sites; therefore the 2.3 ... decrease in NMNATactivity in our mutant mice underlines the lack of anyadverse phenotype. The embryonic lethality of NMNAT1 full inactivation precludes the possibility of testing the rate of Wallerian...

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