Báo cáo khoa học: The fate of newly synthesized V-ATPase accessory subunit Ac45 in the secretory pathway doc
Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot
... pro- tein–protein interaction. In this study, Osh6p was demonstrated to bind a pool of phosphatidylinositol phosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P, PtdIns(3,4)P 2 and PtdIns(3,5)P 2 with ... provided invaluable in- sights into understanding the function of this family of proteins in yeast and offered guidance to future research. On the other hand, although the...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Differential involvement of protein kinase C alpha and epsilon in the regulated secretion of soluble amyloid precursor protein docx
... data in the literature that indicate PKCe as the only protein kinase isoform involved in the s ignalling pathway downstream of muscarinic m3 receptors in SK-N-BE(C) neuroblasto ma cells [15]. The Ó ... PKCe in the r egulated processing of APP. There is substantial evidence in the literature for a significant role of PKCe both in the regulation of APP metabol...
Ngày tải lên: 23/03/2014, 13:20
Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx
... coupling of the two calcium binding sites within G2 and G6. Additional calcium binding in other domains then result in the complete opening and activation of the gelsolin molecule. Keywords: actin; ... to G2 domain. Footprint of G2 on gelsolin G4–6 domain Two sites within the G2 domain appear involved in the G2- G4–6 interface in the presence of EGTA (Fig. 3). The...
Ngày tải lên: 31/03/2014, 01:20
Báo cáo khoa học: Identification of a novel inner-core oligosaccharide structure in Neisseria meningitidis lipopolysaccharide docx
... with the observed differences in the NOE contacts for the Glc I to Hep I linkage. In all three strains examined, in which the Hep I residue of the LPS bears the additional Glc II residue at the ... [10]. The remainder of the anomeric resonances in the low-field region (4.45– 6.00 p.p.m.) of the spectrum were all attributable to b-linked residues by virtue o...
Ngày tải lên: 08/03/2014, 02:20
Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt
... determined the X-ray crystal structure of a truncated form of importin-a lacking the importin-b binding domain, bound to a CLIC4 NLS peptide. The NLS peptide binds to the major binding site in an extended ... cargo binding sites, referred to as the major and minor binding sites [1]. These sites are located in the concave face of the protein near regions of invariant...
Ngày tải lên: 14/02/2014, 19:20
Báo cáo khoa học: A single EF-hand isolated from STIM1 forms dimer in the absence and presence of Ca2+ ppt
... two termini of the inserted Ca 2+ -binding sites in the model structure of the EF-hand of STIM1 is within 15 A ˚ . Accordingly, a total of six glycine linkers is suffi- cient to enable the grafted ... the initial mixture. The fluorescence intensity was normalized by subtracting the contribution of the baseline slope using logarithmic fitting. The Tb 3+ -binding affin...
Ngày tải lên: 07/03/2014, 00:20
Báo cáo khoa học: NMR study of complexes between low molecular mass inhibitors and the West Nile virus NS2B–NS3 protease ppt
... structures of WNV NS2B–NS3pro have been reported in the absence of inhibitor [4] and in the presence of peptide inhibitors [5,6] or bovine pancre- atic trypsin inhibitor (BPTI) [4]. In the absence of inhibitor, ... may be because of spin-diffusion during the mix- ing time of the NOESY experiment, movements of the ligand in the binding pocket or differences...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo khoa học: Combined use of selective inhibitors and fluorogenic substrates to study the specificity of somatic wild-type angiotensin-converting enzyme docx
... composition in the binding site of these three ACE species were pinpointed. Together these data demonstrate that the substrate selectivity of the N-domain and C-domain depends on the ACE species. These ... by the N-domain and C-domain (indicated by the arrow in Fig. 1A). Thus, information on the selectivity of both the sub- strate and the inhibitor for the N-doma...
Ngày tải lên: 16/03/2014, 13:20
Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... its S 1 ¢ binding subsite. Substrate binding induces conformational changes involving the ArgA145- PheA146 fragment of the protein chain, acting as a flap, first opening and then closing the groove ... with the O atom of the main chain CO group of the TrpB240; Ng 1 shares H bonding with O atom of the CO group of LeuB387 together with O atom of c-OH of SerB386; Ng 2 i...
Ngày tải lên: 16/03/2014, 16:20
Báo cáo khoa học: Reducing expression of NAD+ synthesizing enzyme NMNAT1 does not affect the rate of Wallerian degeneration ppt
... within the first 16 amino acids, as their removal results in loss of neuroprotec- tive phenotype [20,21]. Interestingly, the only known binding partner of the N-terminal region, AAA ATPase valosin-containing ... The K14 Cre induces a full dele- tion when bred from the female as K14 is expressed in the oocyte [27]. The offspring of this cross had recom- bination between...
Ngày tải lên: 22/03/2014, 16:20