Báo cáo Y học: Cloning, expression and characterization of a

Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes pot

... Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes Jihui Zhang, Wenbo Ma* and Huarong Tan Institute of Microbiology, ... another catalytic pathway. Mg 2+ and Ca 2+ did not affect NaoA activity, and are therefore probably not necessary for the oxidation. However, Cu 2+ strongly inhibited t...

Ngày tải lên : 31/03/2014, 08:20

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Báo cáo khoa học: Cloning, expression and characterization of a new aspartate aminotransferase from Bacillus subtilis B3 docx

... also have AAT activity, the enzymatic activity of the recombinant AATB3 expressed and puriﬁed from E. coli was analyzed. Native PAGE analysis showed that the wild-type AATB3 had AAT activity ... substrate. Aromatic amino acids such as l-tryptophan, l-tyrosine and l-phenylalanine were weakly active as amino donors, and the activity of transamination activity toward l-tryptophan...

Ngày tải lên : 14/03/2014, 23:20

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Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobiﬁda fusca pptx

... (http://genome.ornl.gov/ microbial/tfus/) and includes many genes coding for a variety of glycosyl hydrolases. One such gene codes for a glycosyl family-74 hydrolase with a typical bacterial family-2 cellulose-binding ... that Xeg74 and presumably all family-74 enzymes catalyze hydrolysis with inversion of the anomeric conﬁguration. This is in contrast to the family-12 A. acu...

Ngày tải lên : 23/03/2014, 21:20

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Báo cáo Y học: Identiﬁcation and characterization of a novel activated RhoB binding protein containing a PDZ domain whose expression is speciﬁcally modulated in thyroid cells by cAMP pot

... epithe- lial morphology correlated with a profound redistribution of both actin microﬁlaments and cytokeratin intermediate ﬁlaments, and with the appearance of a marked cytokeratin and actin immunoreactivity ... rho-binding domain. J. Biol. Chem 271, 13556–13560. 15. Ishizaki, T., Maekawa, M., Fujisawa, K., Okawa, K., Iwamatsu, A. ,Fujita ,A. ,Watanabe,N.,Saito ,Y. ,Kakizuka ,A...

Ngày tải lên : 23/03/2014, 21:20

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Tài liệu Báo cáo Y học: Identiﬁcation and characterization of a mammalian 14-kDa phosphohistidine phosphatase pdf

... Switzerland. Phenylmethanesulfonyl ﬂuoride and okadaic acid were from Sigma. Trypsin of modiﬁed sequencing grade was obtained from Promega, and LysC of Achromobacter lytii from Wako. The O-phosphorylated phosphopeptides ... the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 primer 5¢-ATT...

Ngày tải lên : 21/02/2014, 01:21

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Báo cáo Y học: Identiﬁcation and characterization of a new gene from Variovorax paradoxus Iso1 encoding N -acyl-D-amino acid amidohydrolase responsible for D-amino acid production pdf

... amidohydrolase; A- 6-D45919: Alcaligenes xylosoxydans ssp. xylosoxydans A- 6 N-acyl- D -Asparate amidohydrolase; A- 6-D50061: Alcaligenes xylosoxydans ssp. xylosoxydans A- 6 N-acyl- D -glutamate amidohydrolase; Alicaligenes faecalis-DA1: ... identity and 63.6% similarity), N-acyl- D -glutamate amidohydrolase (44.8% identity and 51.2% similarity) and N-acyl- D -asparate amidohydrolas...

Ngày tải lên : 08/03/2014, 16:20

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Báo cáo Y học: Puriﬁcation and characterization of three galactose speciﬁc lectins from Mulberry seeds (Morus sp.) ppt

... Puriﬁcation and characterization of three galactose speciﬁc lectins from Mulberry seeds ( Morus sp.) Tanzima Yeasmin, Md Abul Kashem Tang, Abdur Razzaque and Nurul Absar Department of Biochemistry, ... the family Moraceae, part of the genus Morus. It is a deep rooted perennial plant, widely distributed in Asia, Europe, Africa and Latin America in a wide range of climatic...

Ngày tải lên : 18/03/2014, 01:20

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Tài liệu Báo cáo Y học: Identiﬁcation and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

... coli. Escherichia coli cells undergo a transition from a rapid growth phase to a stationary phase, which is accompanied by a variety of physiological changes that affect gene expression, the structure and ... 142–144 amino-acids long; they are acidic and presumably located in the cytoplasm like UspA. Members of this family share a strikingly similar hydro- pathy proﬁle...

Ngày tải lên : 22/02/2014, 07:20

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Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt

... proliferation assay We next analyzed the ability of recombinant SSA to stimulate human T-cells. All SSA preparations yielded Fig. 1. SDS/PAGE and immunoblotting analysis of SSA. (A) 12.5% SDS/PAGE of ... several strains of Staphylococcus aureus and Streptococcus pyogenes are structurally and immunologically the best c haracterized to date [6], although the crystal structures of...

Ngày tải lên : 07/03/2014, 16:20

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Báo cáo Y học: Puriﬁcation and characterization of novel kininogens from spotted wolfﬁsh and Atlantic cod pdf

... biantennary and triantennary N-glycans that are terminated by a2 ,3-linked sialic acids, a high number of which are O-acetylated. About 1/3 of the glycans carry sulfate at N-acetylglucosamine units of ... water and analysed by MALDI-TOF MS. Phosphatase treatment Dry N-glycan samples were dissolved in 50 m M ammonium bicarbonate, and 1 U of calf intestinal alkaline phosphatase...

Ngày tải lên : 08/03/2014, 23:20

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