Báo cáo Y học: Modulation of inositol 1,4,5-triphosphate concentration by prolyl endopeptidase inhibition ppt

... Kerenyi, T.D. (1971) Leucylglycinamide released from oxytocin by human uterine enzyme. Science 173, 827–829. 35. Shinoda, M., Miyazaki, A. & Toide, K. (1999) Effect of a novel prolyl endopeptidase ... 9H-fluorenyl-9-ylmethyl N-[2-(2-cyano-1-pyrrolidinyl)-1-methyl-2-oxoethyl]carbamate; NHMec, 7-(4-methyl)coumarylamide; PEP, prolyl endopeptidase. Enzyme: prolyl endopeptidase...

Ngày tải lên: 31/03/2014, 08:20

... Kinetics of inhibition of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase by the novel HIV-1-specific nucleoside analogue [2¢,5¢-bis-O-(tert-butyldimethylsilyl)-beta-D-ribofurano- syl]-3¢-spiro-5¢-(4¢-amino-1¢,2¢-oxathiole-2¢,2¢-dioxide) ... of HIV-1 PR Thr-Val-Ser-Tyr-Glu-Leu-OH 12 Kinetic analysis [20] Palmitoyl-Thr-Val-Ser-Tyr-Glu-Leu-OH 0.5 Kinetic analysis [20]...

Ngày tải lên: 08/03/2014, 09:20

... subsequently desiccated. 2 Coupling of the Fmoc-C2-glycine-tert-butyl ester with the Reppe anhydride: 2 mmol of the Fmoc-C2-glycine-tert-butyl ester 9 and 4 mmol N,N-diisopropylethylamine ... acetic ether were used at the ratio of 2:1. The purity was estimated by use of thin-layer chromatography. Cleavage of the tert-butyl ester. 2 mmol tert-butyl pro- tected PNA building...

Ngày tải lên: 26/10/2012, 08:57

... The importance of hydrophobic interactions in the aggrega- tion of a-synuclein has recently been reinforced by agi- tation studies which have clearly shown the formation of amyloid-type of aggregates only ... of aggregates formed in case of a-synuclein alone and in the presence of MPTP ⁄ MPP + is different. Abbreviations MPP, 1-methyl-4-phenylpyridinum; MPTP, 1-methyl-4-phenyl...

Ngày tải lên: 14/02/2014, 19:20

... mixtures of methyl glycosides were analyzed on GLC [14,15]. The absolute configurations of the monosac- charides were determined by GLC analysis of the trimeth- ylsilylated (–)-2-butyl glycosides ... neutralization and removal of boric acid by coevaporation with methanol, the mixture of partially methylated alditols was acetylated with acetic anhydride (3 h, 120 °C), and analyzed...

Ngày tải lên: 08/03/2014, 09:20

... Neuropharmacology, Laboratory of Neurochemistry, Rome, Italy 4 Laboratory of Pathophysiology of Integrative Autonomic Systems, IRCCS Neurological Institute C. Mondino Foundation and University Centre ... levels of the endocannabinoid anandamide, and the activities of the synthesizing and hydrolyzing enzymes, i.e. N-acylphosphatidyletha- nolamine-hydrolyzing phospholipase D and fatty...

Ngày tải lên: 23/03/2014, 07:20

... Thr70, the sites most profoundly affected by rapamycin, may be regulated by nutrients through the mTOR pathway. REGULATION OF 4E-BP1 BY AMINO ACIDS In a number of kinds of mammalian cells, amino ... the control of eIF4E (by 4E-BP1) for the activation of protein synthesis by amino acids [92]. In isolated rat heart cells (ventricular myocytes), protein synthesis is acutely ac...

Ngày tải lên: 23/03/2014, 21:20

... was stopped by the addition of 250 m M EDTA. The extent of phospholipid hydrolysis was analysed by TLC. RESULTS Construction of a covalent OMPLA dimer The absence of cysteines in wild-type OMPLA ... Whereas wild-type OM- PLA activity displayed high sensitivity towards the type and concentration of detergent, enzymatic activity of the cova- lent OMPLA dimer was insensitive to...

Ngày tải lên: 24/03/2014, 03:21

... mV) and the maximum concentration of semiquinone stabilized by the wild-type enzyme ([SQ] ¼ 22.4% as determined by photoreduction experiments) are known. Thus, by simultaneously solving this Nernst ... as only the methyl groups of the FNR dimethylbenzene ring, proposed to be the entry point of electrons, are exposed to the solvent [20]. Replacement of E301 by Ala increases t...

Ngày tải lên: 08/03/2014, 23:20

... detected by chemiluminescence using a standard ECL kit and developed Hyperfilm ECL film. They were quantified by scanning photodensitometry using ImageQuant Software 3.3 (Molecular Dynamics, Sunnyvale, ... specificity of the antibody to UCP3 was tested using UCP3KO muscle mitochondria, which were described by Gong et al. [7] and generously provided by Dr Mary Ellen Harper (University o...

Ngày tải lên: 24/03/2014, 04:21