Báo cáo Y học: Diffusion through channel derivatives of the Escherichia coli FhuA transport protein doc

... rapid opening and closing of the FhuAD5–160 channel or, less likely, rapid membrane entry and exit of the protein. The instability of the channels formed by FhuAD5–160 may have several causes. It ... and depending on their orientation may modulate the movement of ions through the channel. Furthermore, the b-barrel of FhuAD5–160 may be less rigid than is suggeste...

Ngày tải lên: 31/03/2014, 08:20

... previously shown that at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases considerably, but only transiently [2,18]. Similarly, we observed that the steady-state ... digested with the same enzymes. The resulting plasmid (pET28yQ) was isolated from E. coli HB101 and the identity of the ybdQ insert was confirmed by DNA sequenc...

Ngày tải lên: 22/02/2014, 07:20

... commonly complicated by hyperglycaemia caused by mediators of stress and inflammation. Severity of disease is the main risk factor for development of hyperglycaemia, but not all severely ill ... for tendency to hyperglycaemia that are the root of hospital acquired hyperglycaemia and in the long term lead to development of diabetes. Although the incidence of hospital acqui...

Ngày tải lên: 25/10/2012, 10:02

... experimental example of a nonglycosylated triplet at position Asn70 [48]. The majority of the peroxidases carry one or two putative glycans. Seven appear to be nonglycosylated. Therefore, the high number of glycans ... and others [56]. The targeting features of the propeptide of barley lectin have been analyzed by amino acid substitutions [57]. The unique extension of...

Ngày tải lên: 21/02/2014, 01:21

... shown that the CMP activation of the reverse of synthesis is by a different mechanism from base exchange [20]. Further analysis of the products released from PtdIns by the Arabidopsis enzyme will ... as a source of enzyme. The transformation of E. coli, a host naturally devoid of PtdIns synthase with a single cDNA ensured that the recombinant protein was the only...

Ngày tải lên: 22/02/2014, 04:20

... parallel the effect of the absence of other homologous regulatory subunits of oxygenase complexes, like T4MOD [33] and component B of methane monooxygenases [38,39]. In these cases the absence of the ... shown) that based on the expected molecular size of the polypep- tides, all the proteins of interest were present in the soluble fraction of the induced cell...

Ngày tải lên: 17/03/2014, 10:20

... judged by the ratio of the integral intensities of the signals in the major and minor series, the average degree of glycosylation with Glc II is  75%. Comparison of the 13 C NMR spectra of the initial ... spectrum of the O-deacetylated polysaccharide compared to those at d 4.70 and 4.62 in the spectrum of the initial polysaccharide (a deshielding effect of...

Ngày tải lên: 17/03/2014, 17:20

... yeast Cdc6p is phosphorylated and then rapidly destroyed b y ubiquitin-mediated protein degradation. The regulated destruction of Cdc6p effectively prevents the binding of Mcm proteins, and therefore ... importantly in th e present context, we note that the synth esis of hCdc6p c ontinues through m ost of the cell cycle. Synthesis of hCdc6p during G1 phase is necessary...

Ngày tải lên: 17/03/2014, 23:20

... hydrolases (EC 3.1.1.3) that catalyze the hydrolysis of triacylglycerols to free fatty acids and glycerol. They resemble esterases in catalytic activity, but differ in that their substrates are water-insoluble ... factors [3] and their biotechnological potential. Lipases have proven to be versatile enzymes in nonaqueous solvent systems in which they catalyze the synthesis of a variety...

Ngày tải lên: 23/03/2014, 21:20

... to the theoretical molecu- lar mass of the protein and to the protein synthesized in vitro in rabbit reticulocyte lysates. Interestingly, the levels of receptor protein vary considerably throughout ... splicing of the two 5¢ exons of the gene (see below). The 3¢ end of the sequence is not supported by the presence of a classical consensus polyadenylation si...

Ngày tải lên: 23/03/2014, 21:20