... previouslyshown that at the beginning of the stationary phase the rate of synthesis of heat-shock proteins increases considerably,but only transiently [2,18]. Similarly, we observed that the steady-state ... digested with the same enzymes. The resulting plasmid (pET28yQ) was isolated from E. coli HB101 and the identity of the ybdQ insert was confirmed byDNA sequencing. Finally, E. coli BL21(DE3)pLysS wastransformed ... at the molecular level. All the E. coli members of the UspA familyare encoded by monocystronic genes dispersed throughout the chromosome, but unlike many other stress-relatedE. coli genes, their...