Báo cáo khoa học: Thioredoxin reductase from the malaria mosquito Anopheles gambiae Comparisons with the orthologous enzymes of Plasmodium falciparum and the human host pdf

... reductase from the malaria mosquito Anopheles gambiae Comparisons with the orthologous enzymes of Plasmodium falciparum and the human host Holger Bauer1, Stephan Gromer1, Andrea Urbani2, Martina ... AAB35418), and from the malaria parasite Plasmodium falciparum (PfTrxR-1, CAA60574). The enzyme of Anopheles shares69%, 52%, and 45% sequence identity with the TrxRs of D. melanogaster, humans, and ... (Figs 3 and 4). The reducing equivalentsﬂow from the nicotinamide of NADPH via the ﬂavin and the pair Cys57/Cys62 to the redox centre Cys500’/Cys501’ of the other subunit, and hence to the disulﬁde...

Báo cáo khoa học: LmbE proteins from Bacillus cereus are de-N-acetylases with broad substrate speciﬁcity and are highly similar to proteins in Bacillus anthracis pot

... interaction between the b8-strand of one monomer and the b6- and b7-strands of the other monomer. (B) Surface representation of the enzyme and view of the active site from outside. The active site ... underlined H and D residues in the ﬁrst motif and the last H of the second motif are zincbinding ligands. Moreover, it has been proposed forother members of the family that the underlined H and the ... may driveacetate out of the active site after the end of the reac-tion. The positioning of a negatively charged residue(E140) could hamper the release of the acetate from the active site. This...

Báo cáo khoa học: Methylcitrate synthase from Aspergillus fumigatus Propionyl-CoA affects polyketide synthesis, growth and morphology of conidia ppt

... P10). The mean value of the diameter of inhibition zones and the deviation of three independent zones isgiven. D from mean gives the difference of the inhibition zones of a single strain from the ... case of the pksP mutant regardless of the car-bon sources the spores derived from. Interestingly, the wild type slightly altered the appearance of the surface of conidia in the presence of propionate ... different degree of disruption of the mycelium and some loss of the acyl-CoA during the puriﬁcationprocedure. Anyhow, an approval of the procedure with known concentrations of acetyl-CoA and propionyl-CoA...

Báo cáo khoa học: "Smaller Alignment Models for Better Translations: Unsupervised Word Alignment with the 0" potx

... (1) The parameters of these models are the distortionprobabilities d(aj| aj−1, j) and the translation prob-abilities t( fj| eaj). The three models diﬀer in theirestimation of d, but the ... the same t. For further details of these models, the reader is referred to the original papers describ-ing them (Brown et al., 1993; Vogel et al., 1996).Let θ stand for all the parameters of ... Chinese-English, and Urdu-English from the NIST Open MT Evaluation, and the Czech-English translation from the Work-shop on Machine Translation (WMT) shared task.We measured the accuracy of word alignments...

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... the ﬁrsthypothesis and therefore a direct involvement of PHI inelectron transfer. This conclusion is consistent with the verylow degree of identity between the N-terminal sequence of PHI and ... nm.N-terminal sequence The ﬁrst 11 aminoacids at the N-terminus of PHI (sequence:SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two otherPHs. The N-terminal ... atk ¼ 200 nm (the absorption region of the peptide bond)reﬂects the occurrence of a transition between 35° and 55 °C.PHI does not interact with phenol The emission spectrum of the protein at...

Báo cáo khoa học: "Speech Technology: from Research to the Industry of Human-Machine Communication" potx

...

Báo cáo khoa học: "Deep dependencies from context-free statistical parsers: correcting the surface dependency approximation" pptx

... daughters; the marking of certainnodes as dislocated; or the relocation of dislocatednodes to other positions within the tree. The content and ordering of phases is consistent with the syntac-tic theory ... compared with the work of Johnson (2002) and Dienes and Dubey(2003a) on WSJ. Valid comparisons exist for the insertion of uncoindexed empty nodes (COMP and ARB-SUBJ), identiﬁcation of control and ... secondwas the ambiguity that some matrix S-initial NPsare actually dependents of the VP head (in thesecases, NEGRA annotates the ﬁnite verb as the head of S and the non-ﬁnite verb as the head of VP)....

Báo cáo khoa học: Methylthioadenosine phosphorylase from the archaeon Pyrococcus furiosus docx

... concentration. In the insets a re reported linear replots of the slopes and of the intercept s of plot (A) vs. the r eciprocal of con centr atio ns of MTA. (B) Plo t of the reciproc al of initial velocity ... in the absence ( r)andinthepresenceof50lM(j )and2 00lM(m)ribose1-phospate. In the insets are shown the replot s o f the slopes and the intercep ts vs. the con centration of t he inhibitor. The ... to the re ciprocal of MTA concentration in the absence(r) and presence of 20 lM(j )and5 0lM(m) adenine. The inh ibition co nstants Kis and Kiiestimated from the rep lots o f the slopes and...

Báo cáo khoa học: Aspartate transcarbamylase from the hyperthermophilic archaeonPyrococcus abyssi Insights into cooperative and allosteric mechanisms docx

... kDa) and bovine serum albumin (67 kDa) were deter-mined. A standard curve was made with the logarithm of the molecular mass of the proteins in the y-axis and the elu-tion volumes (Ve) in the ... consequence of the cooperativity toward aspartate and of the orderedbinding of the substrates, ﬁrst CP then aspartate [13].These results raised the possibility that binding of the substrates ... Comparison of the elutionvolumes of the P. abyssi and E. coli ATCases with the standard curve gave an estimate of their molecular mass.Enzyme assaysATCase activity was assayed using the colorimetric...

Báo cáo khoa học: Procarboxypeptidase A from the insect pest Helicoverpa armigera and its derived enzyme potx

... Analysis of the species generated during the activation process. The activation of PCPHa with LysC was performed in the conditions of Fig. 2B with the addition of the carboxypeptidase inhibitor from ... in the meth-ylotrophic yeast Pichia pastoris indicates both the suitability of this organism to host the heterologous expression of thisclass of enzymes [23,24] and the correct folding of the proenzyme. ... Fig. 4B the analysis of the species isolated from the chromatograms in part A of the ﬁgure conﬁrms that the protein expressed in the Pichia pastoris system is glycosyl-ated, and that the glycosylation...

Xem thêm