Báo cáo khoa học: Demethylation of radiolabelled dextromethorphan in rat microsomes and intact hepatocytes Kinetics and sensitivity to cytochrome P450 2D inhibitors pot

... Accumulation in rat hepatocytes and hepatic protein binding of quinine and quinidine. Accumulation of quinine and quinidine (5 l M ) in rat hepatocytes is expressed as the steady state ratio (C/M) ... 3777 Demethylation of radiolabelled dextromethorphan in rat microsomes and intact hepatocytes Kinetics and sensitivity to cytochrome P450 2...

Ngày tải lên: 31/03/2014, 07:20

... rapidly and the propensity to err rapidly increases after 16 hours of sustained wakefulness, a finding reflected in the twofold increase in interns’ attentional failures after they had been working ... frequency of extended-duration work shifts, but on the duration of consecutive work hours during such shifts. Research from laboratory and industrial settings suggests that perf...

Ngày tải lên: 25/10/2012, 10:45

... shown that interactions between KCs and endotoxin comprise the initiating event lead- ing to hepatotoxicity in liver injury, including endotox- emia and ischemia ⁄ reperfusion injury [14]. In our studies, ... KCs [25]. The expression and activity of CYP2E1 were downregulated in a rat hepatoma cell line after the administration of proinflammatory cytokines, leading to a lo...

Ngày tải lên: 14/02/2014, 18:20

... Exposure of a variety of cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proin- flammatory cytokines, inhibition of insulin signaling, induction ... translocation, indicating the involvement of Bax activation in stearate-induced mitochondrial destabilization and apoptosis. In order to further evaluate specific m...

Ngày tải lên: 14/02/2014, 22:20

... used the in situ rat liver model system following toxin adminis- tration to rats and cell-free hepatic endosomes to relate the endosomal processing of internalized ETA to toxin cytotoxicity in a ... time activation of the intrinsic apoptotic pathway with ETA cytotoxicity in a physiological state. Results In vivo endocytosis and metabolic fate of ETA in rat liver...

Ngày tải lên: 18/02/2014, 04:20

... Cer is incorporated, hydrolyzed to sphingosine and then recycled into intracellular Cer itself, and that accumu- lation of Cer contributes to the induction of apoptosis [20]. However, the signaling ... cultured human keratinocytes [14]. In this study, we examined the interaction between CerK and PPARb, and its role in regulating keratino- cyte survival. We report that in...

Ngày tải lên: 18/02/2014, 18:20

... exam- ine the roles of the conserved arginines, Arg322 (P2) and Arg323 (P1) of AAA-1, and Ar g747 (P1) of AAA- 2, and determined that the arginines of TClpB are not involved in nucleotide binding ... of the arginines in ATP binding and hydrolysis in each module. The arginine mutations did not affect nucleotide binding to the mutated AAA+ module (Fig. 2; Table 1) but...

Ngày tải lên: 06/03/2014, 00:20

... group of the tissue inhibitor of metalloproteinases-2 NH 2 -ter- minal cysteine has been used to demonstrate the key role of this amino group in the inhibitory effect of tissue inhibitor of metalloproteinases-2 ... from jararhagin disin- tegrin) is essential for binding to the I domain of a 2 integrins [24]. Similarly, members of the ‘lysine–threo- nine–serine (KTS)–disint...

Ngày tải lên: 07/03/2014, 06:20

... De-O-acylation of lipid A fractionsledtodecayofspeciesZandYandresultedin increase of signals corresponding to ions of species X (data not shown). The total decrease of mass due to de-O- acylation of phosphorylated ... linkages to the backbone glucosamines. Lipid A is responsible for the endotoxic properties of lipopolysaccharide. The structure of lipid A seems to be esse...

Ngày tải lên: 07/03/2014, 15:20

... McLaughlin S & Smith SO (2003) Binding of Peptides with Basic and Aromatic Residues to Bilayer Membranes: Phenylalanine in the myristoylated Alanine-rich C kinase substrate effector domain penetrates ... Interactions con- trolling the membrane binding of basic protein domains: phenylalanine and the attachment of the myristoylated alanine-rich C-kinase substrate protein to...

Ngày tải lên: 07/03/2014, 17:20