Báo cáo khoa học: Transient increase of the labile iron pool in HepG2 cells by intravenous iron preparations Brigitte Sturm, Hans Goldenberg and Barbara Scheiber-Mojdehkar doc

... Transient increase of the labile iron pool in HepG2 cells by intravenous iron preparations Brigitte Sturm, Hans Goldenberg and Barbara Scheiber-Mojdehkar Department of Medical Chemistry, ... The initiation of translation of ferritin by an increase in the labile iron pool (LIP) and the subsequent incorporation of labile i...

Ngày tải lên: 31/03/2014, 07:20

... interactions of papain with loop L6 of chagasin and loop L2 of cysta- tin B. (C) Zoom -in view of the interactions of papain with loop L4 of chagasin and the N-terminal segment of cystatin B. (D) Zoom -in ... are interpreted as further improving the binding efficiency to papain and cathep- sin L versus the other enzymes, with the hydrogen bond between D99 in l...

Ngày tải lên: 16/03/2014, 04:20

... process, the ENA ⁄ VASP family proteins bind to both the barbed end and sides of actin filaments [15–18]. These actin-binding activities are conserved in the EVH2 domain [19,20]. On the other hand, the ... domain binds to proteins containing the consensus sequence D ⁄ E FPPPPXD ⁄ E [21–23]. The Pro-rich domain also binds to profilin and proteins containing the SH3 and...

Ngày tải lên: 23/03/2014, 04:20

... interactions between the subunits and are involved in cofactor binding, substrate binding and catalysis (Fig. 1). On the other hand, the mutations did not affect either the capability to bind the cofactor or the ... conditions indicated that the stability of the cluster is essential for the correct quaternary assembly of the enzyme and is increased by the b...

Ngày tải lên: 30/03/2014, 02:20

... IscU. The NifU protein from Azotobacter vinelandii consists of three domains. The N-terminal domain is very similar in sequence and size to IscU, the central domain is similar to a protein carrying ... to change the codons of the Cys residues to Ala. The presence of the mutations were verified by digestion with the enzymes cleaving the introduced restriction sites...

Ngày tải lên: 31/03/2014, 01:20

... which is composed of N-terminal A ⁄ B domain, the DNA binding C domain, the D domain (hinge region), ligand binding E domain, and C-ter- minal F domain. EcR heterodimerizes with other Keywords ChIP ... domain and VP16 activation domain. Various combinations of the EcR ligand binding domain, VP16 activation domain and GAL4 DNA binding domain were constructed (Fig. 1A) and tes...

Ngày tải lên: 16/03/2014, 12:20

... also examined the relative depth of peptide insertion into negative and zwitterionic lipid bilayers by comparing the dose–response curves of the colorimetric transitions induced by the peptides within ... within the vesicles promotes deeper insertion of melittin and KAL, but does not seem to affect the strong interfacial binding of magainin. For example, at peptide...

Ngày tải lên: 30/03/2014, 20:20

... an independent metal-binding site at the interface of the two domains, which consists of four side chains: two (His31 and His79) from the N-terminal domain and two (Asp165 and His169) from the ... residues involved in the binding of the metal cofactor. ApeSODs in different forms were superimposed with the least square fit of Ca atoms of the residues in th...

Ngày tải lên: 14/02/2014, 22:20

... [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo assembly of the metallocofactor of C. ammoniagenes R2F is ... samples. The rad- ical concentration was calculated by determining the area of the radical signal in the absorption spectrum by cubic spline interpolat...

Ngày tải lên: 15/02/2014, 01:20

... the ACT1 domain is inserted into the ACT2 domain; and (c) two ACT domains, each from a different chain, interact to form an effec- tor-binding unit. The effector-binding unit of the ACT domain ... model. Both ACT domains forming an effector-binding unit of the front of the dimer are indicated. Site 1 and site 2 of the effector-binding unit on the front are indicate...

Ngày tải lên: 18/02/2014, 08:20