Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
Báo cáo khoa học: Kinetic studies on endo-b-galactosidase by a novel colorimetric assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity pptx
... Consecutive additions of Glc-NAc and Gal to Galb1-4GlcNAcb-pNP by b3GnT and b4GalT. (B)Consecutive additions of GlcNAc and Gal to Galb1-4Glcb-pNP by b3GnT and b-D-galactosidase or b4GalT. (C) N- acetylglucosaminy-lation ... mg) based on the acceptor from 3 and UDP-Gal.1Hand13C-NMR data of 2 and 4 are summarized inTable 1.Preparation of GlcNAcb1-3Galb1-4GlcNAcb-pNP (5) and its positional isomer GlcNAcb1-3Galb1-4GlcNAcb-pNP ... assay and synthesis of N -acetyllactosamine-repeating oligosaccharide b-glycosides using its transglycosylation activity Takeomi Murata, Takeshi Hattori, Satoshi Amarume, Akiko Koichi and Taichi...
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Tài liệu Báo cáo khoa học: Efficient RNA ligation by reverse-joined hairpin ribozymes and engineering of twin ribozymes consisting of conventional and reverse-joined hairpin ribozyme units ppt
... is not as efficient as its tandem-configured relative [16], its successful construction and the demonstration of its functionality supports the idea of creating novel RNA catalysts by rational ... functional RNA, for example, sig-nalling aptamers and aptazymes [8–10].Work in our laboratory has focused on the rationaldesign of functional RNA, in particular on thedevelopment of hairpin-derived ... enhancedcoaxial stacking of the two domains in the relevantvariant of the hinged conventional hairpin ribozymeleading to enrichment of an extended inactive confor-mation [34,35]. Because of...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf
... amounts of Akazara scallop TnC. Lanes h and m, ATnI-52K; lanesi and n, ATnI-19K; lanes j and o, ATnI1)128; lanes k and p, ATnI130)252; lanes l and q, ATnI232)292; lane r, Akazara scallop ... then run on SDS ⁄ PAGE. Lanes a and d, inthe absence of both TnC and Ca2+; lanes b and e, in the presence of TnC and the absence of Ca2+; lanes c and f, in the presence of bothTnC and Ca2+. ... known to interact with TnC inan antiparallel manner such that the regulatory and Fig. 6. Ca2+-regulation of actomyosin-tropo-myosin Mg-ATPase by rabbit (A and C) and Akazara scallop (B and...
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf
... hand, the determined catalytic constants are apparentones, with varying relative contributions of individual rateconstants and their discussion is not straightforward.An important detail of ... biocatalysts often requires transformations of substrates not encountered in nature under reaction condi-tions differing from physiological ones. Protein designaimed at rational optimization and/ or ... screening of protein engineered PAfor new enzymatic transformations, and analysis of kineticsCorrespondence to N. Stambolieva or J. Kaneti, Institute of OrganicChemistry, 1113 So a, Bulgaria....
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Báo cáo khoa học: Mechanistic studies on bovine cytosolic 5¢-nucleotidase II, an enzyme belonging to the HAD superfamily doc
... recombinant cN-II assay. Accordingly, an alter-ation o f t his r atio for a mutant was considered as beingcaused either by an alteration of the Kmvalue for one of thetwo s ubstrates or by a variation ... thrombin cleavage site.The p ro tein concentration w as determined according toBradford [30], using BSA as a standard. The molarconcentration of the enzymes was determined by using thecalculated ... studies on bovine cytosolic 5¢-nucleotidase II, an enzymebelonging to the HAD superfamilySimone Allegrini1,*, Andrea Scaloni2,*, Maria Giovanna Careddu1, Giovanna Cuccu3, Chiara D’Ambrosio2,Rossana...
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Báo cáo khoa học: NMR studies on the interaction of sugars with the C-terminal domain of an R-type lectin from the earthworm Lumbricus terrestris pot
... Hirabayashi21 National Food Research Institute, National Agriculture and Food Research Organization (NARO), Tsukuba, Japan2 Research Center for Medical Glycoscience, National Institute of Advanced Industrial ... theconfiguration at the hemiacetal carbon of galactosemay affect the dissociation constants. By contrast, because chemical-shift changes uponthe addition of sugars at subdomain c were in the fastexchange ... C-terminal domain of EW29 at pH 6.1 and 298K in the sugar-freestate. Cross-peaks are labeled based on ananalysis of through-bond connectivities. Theside chains of NH2resonances of aspara-gines...
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Tài liệu Báo cáo khoa học: "Three BioNLP Tools Powered by a Biological Lexicon" doc
... percentage of the terminological words and derivational relations (such as the word retroregulate and the derivational relation retroregulate → retroregulation) in our lexicon that are also ... …”IL/NPIL-2/NN-BIOMED-/-2/CDmediated/VVDIL-2-mediated/UNKNOWNIL/NP2/CDIL-2/NN-BIOMEDＢｉｏＬｅｘｉｃｏｎmediated/VVDmediate/VVPmediate/VV of/ INmediated/VVN-/--/-mediated/VVNdictionary-based tagging of/ INFig. 3 BLTagger example coverage020406080100verbnounadjectiveadverbnominalizationadjetivial adverbalTerminologies Derivational relations ... found in WorNet and the Specialist Lexicion. Since WordNet is not targeted at the biology domain, many biological terms and derivational relations are not included. Because the Specialist...
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Báo cáo khoa học: "Machine Aided Error-Correction Environment for Korean Morphological Analysis and Part-of-Speech Tagging" pptx
... '~(saram)/ncn+da'un/ncpa', '2 ,-7, (hag'gyo)/ncn+da'un/ncpa'. Correction rule can apply to the many kinds of word phrase; while manual log is concerned about only one instance ... transformation rules dealing with agglutinative characteristics of Korean, and also extends the tagger by using specific transforma- tion rule considering the lexical information of mistagged ... Machine Aided Error-Correction Environment for Korean Morphological Analysis and Part -of- Speech Tagging Junsik Park, Jung-Goo Kang, Wook Hur and Key-Sun Choi Center for Artificial Intelligence...
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Báo cáo khoa học: Molecular mechanisms regulating molting in a crustacean Halagowder Devaraj and Ayithan Natarajan ppt
... because of its nonseasonal availability and easy main-tenance. They were obtained from the coastal region of Kovalam, Chennai, India. They were maintained in thelaboratory at a temperature of 28 ... Molecular mechanisms regulating molting in a crustaceanHalagowder Devaraj and Ayithan NatarajanUnit of Biochemistry, Department of Zoology, University of Madras, Tamil Nadu, IndiaPeriodic shedding ... indicus. These are the firstdata showing an association between the Gas7 signal-transduction process and regulation of the molt cycle and provides an alternative molecularintervention mechanism...
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Báo cáo khoa học: Alpha 1-antichymotrypsin/SerpinA3 is a novel target of orphan nuclear receptor Nur77 potx
... (transactivation function 1,AF-1), a DNA-binding domain, and a ligand-bindingdomain. Nur77 has no specific ligand discovered hith-erto. The crystal structure of the ligand-bindingdomain of Nurr1, another ... SerpinA3 (1 : 50 dilution in NaCl/Pi)containing 3% BSA at 4 °C overnight, washed abundantlyin NaCl/Pi, and incubated for 1 h with the TRITC-conju-gated secondary antibody. The final concentration(10 ... plasmids wereconfirmed by DNA sequencing by Beijing Nuosai GeneCompany (Beijing, China).Transient transfection and luciferase assay Cells were transiently transfected with plasmids by using Lipofectamine...
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