Báo cáo khoa học: Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling Delivered on 24 October 2002 at the 28th FEBS Meeting in Istanbul potx

... THE SIR HANS KREBS LECTURE Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling Delivered on 24 October 2002 at the 28th FEBS Meeting in Istanbul Jacques Pouysse ´ gur and ... by the serine/threonine kinase, C-TAK1 that phosphorylates KSR1 at a site that confers 14-3-3 binding, thus sequestering the KSR1 complex in the...

Ngày tải lên: 31/03/2014, 07:20

... radical and cationic species hinted at the interference of cationic r earrangements, predicting the hydroxylation to occur via an insertion reaction in place o f abstraction and recombination [9]. The former ... spin-paired low-spin ensemble and a nonspin- paired high-spin manifold, with state crossing being asso- ciated with either an insertion or a radicalar pathway, permitt...

Ngày tải lên: 19/02/2014, 16:20

... linear in terms of both incubation time (up to 90 min) and protein concentration (0.5–5 lgprotein;data not shown). Maximum enzyme activity (V max )atsteady- state conditions and saturation concentrations ... cent inhibitory concentrations (IC 50 ) were determined from the inhibition curves. Ó FEBS 2003 L. donovani adenylate kinase (Eur. J. Biochem. 270) 4341 Results Cloning and...

Ngày tải lên: 21/02/2014, 00:20

... present in all strains, inde- pendent of the origin of the cloned cDNA and whether the enzymes are naturally located in mitochondria. However, the data do not demonstrate the proper tar- geting of the ... many other proteins in each fraction, including those rich in IRP1. To gain insight into the possibility of coprecipitation of proteins with IRP1 and to assess poss...

Ngày tải lên: 07/03/2014, 10:20

... obtained from the overexpression host is approximately 20% holoenzyme. After reconstitution with FAD the enzyme contains 0.92 flavin per subunit based on the ratio of protein concentration to flavin ... of the flavin isoalloxazine ring with the C4 position of the nicotina- mide ring located 3.49 A ˚ from N5 of the isoalloxazine ring. The active site cysteine thiolate is 3.48 A...

Ngày tải lên: 07/03/2014, 17:20

... subdomains. Localization of the substrate binding pockets is analogous to that of hexokinase, with the ATP pocket in the adenosine sub- domain and the sugar pocket on the opposite side within the b-sheet ... localization of the cysteine residues, a three-dimensional model of glucokinase was used [23]. Based on the sequence align- ments of glucokinase and GlcNAc kina...

Ngày tải lên: 08/03/2014, 10:20

... revealed another important PtdIns3P effector in endosomal tethering and fusion, namely EEA1, a protein that contains a Rab5 binding domain and a FYVE domain at its C-terminus, and a distinct Rab5 binding ... domain-containing proteins and some 45 PX domain-containing proteins that presumably mediate most (but not all) of the downstream functions of PtdIns3P [29]. Additional PtdI...

Ngày tải lên: 06/03/2014, 01:23

... ACE-I and statins might be explained by their stimulatory effect on the endothelial generation of PGI 2 and NO • . There are other propositions concerning the mechan- ism of endothelial actions ... hydrophilic pravastatin) at a concentration of 30 lm mobilize free cytoplasmic calcium [Ca 2+ ] i to 30–50% of that induced by Bk at a concentration of 10 nm. In the case of simva...

Ngày tải lên: 07/03/2014, 21:20

... this question. In particular, I was interested in determining the role of CDKs in the regulation of mitotic Clb cyclin degradation, by examining the consequences of CDK inactivation on mitotic Clb cyclin ... Clb-CDKs induced to phosphory- late the inhibitor? Interestingly, the FEAR network components Esp1 and Slk19 do not promote activation of the kinase but rather ina...

Ngày tải lên: 23/03/2014, 06:20

... premature coding sequence termination if it contained an in- frame stop codon within an internal exon. For the predicted patterns of splicing, there is redundance in the number of accessions shown ... genera- tion of new U2AF-related proteins. The similarity and differences between the U2AF-related proteins imply that they have evolved distinct functions in relation to the c...

Ngày tải lên: 23/03/2014, 10:20