Báo cáo khoa học: Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling Delivered on 24 October 2002 at the 28th FEBS Meeting in Istanbul potx

... THE SIR HANS KREBS LECTURE Fidelity and spatio-temporal control in MAP kinase (ERKs) signalling Delivered on 24 October 2002 at the 28th FEBS Meeting in Istanbul Jacques Pouysse´gur and ... by the serine/threonine kinase, C-TAK1 thatphosphorylates KSR1 at a site that confers 14-3-3 binding,thus sequestering the KSR1 complex in the cytoplasm in the absence of stimulation [22]. In ... extinc-tion during the S-phase. These features are exquisitely con-trolled by the temporal induction of the MAP kinase phosphatases, MKP1–3. MKP1 and 2 induction is strictlycontrolled by the...

Tài liệu Báo cáo khoa học: Models and mechanisms of O-O bond activation by cytochrome P450 A critical assessment of the potential role of multiple active intermediates in oxidative catalysis doc

... radical and cationicspecies hinted at the interference of cationic r earrangements,predicting the hydroxylation to occur via an insertionreaction in place o f abstraction and recombination [9]. The former ... spin-paired low-spin ensemble and a nonspin-paired high-spin manifold, with state crossing being asso-ciated with either an insertion or a radicalar pathway,permitting the formation of cationic ... [102] there is lack of a conservedthreonine near the putative O2-binding site. This opens the possibility that threonine mutagenesis rather induces acompensatory change in the organized, H-bonded...

Tài liệu Báo cáo khoa học: Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani pdf

... linear in terms of both incubation time (up to90 min) and protein concentration (0.5–5 lgprotein;datanot shown). Maximum enzyme activity (Vmax)atsteady-state conditions and saturation concentrations ... cent inhibitory concentrations (IC50)were determined from the inhibition curves.Ó FEBS 2003 L. donovani adenylate kinase (Eur. J. Biochem. 270) 4341ResultsCloning and characterization of the AKgene in L. ... binding of the substrate is shown in the top-mostportion of the protein. ATP may bind to the glycine loop on the left ﬂap portion (Fig. 8B) and AMP may bind to the AMP ﬂap shown on the right...

Báo cáo khoa học: Folding and turnover of human iron regulatory protein 1 depend on its subcellular localization pdf

... present in all strains, inde-pendent of the origin of the cloned cDNA and whether the enzymes are naturally located in mitochondria.However, the data do not demonstrate the proper tar-geting of the ... many other proteins in each fraction, includingthose rich in IRP1. To gain insight into the possibilityof coprecipitation of proteins with IRP1 and to assesspossible nonspeciﬁc binding, attempts ... aremetalloproteins containing a [4Fe)4S] cluster thatbinds citrate or isocitrate and acts as a Lewis acid toisomerize these substrates. Aconitases catalyse onereaction of the citric acid cycle and they...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... obtainedfrom the overexpression host is approximately 20%holoenzyme. After reconstitution with FAD the enzyme contains 0.92 ﬂavin per subunit based on the ratio of protein concentration to ﬂavin ... of the ﬂavinisoalloxazine ring with the C4 position of the nicotina-mide ring located 3.49 A˚from N5 of the isoalloxazinering. The active site cysteine thiolate is 3.48 A˚fromN5 on the ... sonicating for3 · 30 s on ice, followed by reconstitution with FAD(1.0 mm )at8 5°C for 10 min. E. coli proteins that precipi-tated during the heat treatment were removed by centrifu-ging in...

Báo cáo khoa học: Structure and function of N-acetylglucosamine kinase Identiﬁcation of two active site cysteines pptx

... subdomains. Localizationof the substrate binding pockets is analogous to that ofhexokinase, with the ATP pocket in the adenosine sub-domain and the sugar pocket on the opposite side within the b-sheet ... localizationof the cysteine residues, a three-dimensional model ofglucokinase was used [23]. Based on the sequence align-ments of glucokinase and GlcNAc kinase and the commonATP-binding domain, ... directly involved in binding the tworelevant functional groups of the kinase reaction, the c-phosphate of ATP and the hydroxyl group at C6 ofGlcNAc, or they may even be involved in the catalyticmechanism.DISCUSSIONThe...

Báo cáo khoa học: How a lipid mediates tumour suppression Delivered on 29 June 2010 at the 35th FEBS Congress in Gothenburg, Sweden pdf

... revealedanother important PtdIns3P effector in endosomaltethering and fusion, namely EEA1, a protein thatcontains a Rab5 binding domain and a FYVE domain at its C-terminus, and a distinct Rab5 binding ... domain-containing proteins and some45 PX domain-containing proteins that presumablymediate most (but not all) of the downstream functionsof PtdIns3P [29]. Additional PtdIns3P-binding proteinsthat ... [30,31], and certain variant pleckstrin homology domains suchas the GLUE (GRAM-like ubiquitin-binding in EAP45) domain [32–34].Intracellular localization of PtdIns3P The identiﬁcation of PtdIns3P-binding...

Báo cáo khoa học: Pharmacology of vascular endothelium Delivered on 27 June 2004 at the 29th FEBS Congress in Warsaw pptx

... ACE-I and statins might be explained by their stimulatoryeffect on the endothelial generation of PGI2 and NO•.There are other propositions concerning the mechan-ism of endothelial actions ... hydrophilicpravastatin) at a concentration of 30 lm mobilize freecytoplasmic calcium [Ca2+]ito 30–50% of thatinduced by Bk at a concentration of 10 nm. In the case of simvastatin and lovastatin, this ... endothelial dysfunction in patients withatherosclerosis [8]. The great expectations for the therapeutic use of antioxidants in patients with athero-sclerosis need to be re-examined.Endothelium...

Báo cáo khoa học: A decade of Cdc14 – a personal perspective Delivered on 9 July 2007 at the 32nd FEBS Congress in Vienna, Austria pdf

... thisquestion. In particular, I was interested in determining the role of CDKs in the regulation of mitotic Clb cyclindegradation, by examining the consequences of CDKinactivation on mitotic Clb cyclin ... Clb-CDKs induced to phosphory-late the inhibitor? Interestingly, the FEAR networkcomponents Esp1 and Slk19 do not promote activationof the kinase but rather inactivate the protein phos-phatase ... provided insight intohow Bub2–Bfa1 activity is regulated by the spindleposition checkpoint. The protein kinase Kin4 phospho-rylates Bfa1. This precludes phosphorylation and henceinactivation of the...

Báo cáo khoa học: Diversity of human U2AF splicing factors Based on the EMBO Lecture delivered on 7 July 2005 at the 30th FEBS Congress in Budapest pptx

... premature coding sequence termination ifit contained an in- frame stop codon within an internal exon. For the predicted patterns of splicing, there is redundance in the number of accessions shown ... genera-tion of new U2AF-related proteins. The similarity and differences between the U2AF-related proteins implythat they have evolved distinct functions in relation to the control of gene expression ... speciﬁcities, and identifying the genes that they control. Ultimately, understanding the function of the diverse U2AF proteins will require that their roles in shaping human development and physiology...

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