Báo cáo khoa học: Transglutaminase from Streptomyces mobaraensis is activated by an endogenous metalloprotease pot

... Transglutaminase from Streptomyces mobaraensis is activated by an endogenous metalloprotease J. Zotzel, P. Keller and H L. Fuchsbauer Fachbereich Chemie- ... Fachhochschule Darmstadt, Germany Streptomyces mobaraensis secretes a Ca 2+ -independent transglutaminase (TGase) that is activated by removing an N-terminal peptide from a precursor protein during ......

Ngày tải lên: 31/03/2014, 07:20

... (2003) Transglu- taminase from Streptomyces mobaraensis is activated by an endogenous metalloprotease. Eur. J. Biochem. 270, 3214–3222. 2. Nicieza, R.G., Huergo, J., Connolly, B.A. & Sanchez, ... pNA, p-nitroanilide; SM, Streptomyces mobaraensis; SSI, Streptomyces subtilisin inhibitor; TAMEP, transglutaminase- activating metalloprotease; TAP, tripeptidyl aminopepti...

Ngày tải lên: 31/03/2014, 07:20

... examples from the mean. 4.2 Mean reciprocal rank A bug report triaging agent is most likely to be used in a semi-automatic workflow, where a hu- man triager is presented with a ranked list of possible ... many sparse features, and is especially useful in learning from text from fast evolving data. The features such as unigram and bigram counts that we rely on are notoriously sparse...

Ngày tải lên: 24/03/2014, 03:20

... translations of the English noun channel are listed in Table 1. To illustrate, if the sense of an occurrence of the noun channel is “a path over which electrical signals can pass”, then this ... translation candidates: Sometimes, multiple and distinct Chi- nese translations appear in the aligned Chinese sentence. For example, for an English occurrence channel, both “频道” (sense...

Ngày tải lên: 08/03/2014, 04:22

... dipeptide anserine is transported by human proton-coupled peptide transporters Stefanie Geissler 1 , Madlen Zwarg 1 , Ilka Knu ¨ tter 1 , Fritz Markwardt 2 and Matthias Brandsch 1 1 Membrane Transport ... experiments show that anserine and car- nosine are transported by hPEPT1 in an active, elec- trogenic manner by an H + symport. As PEPT1 is the predominant transport system for...

Ngày tải lên: 15/03/2014, 09:20

... apoptosis, cell survival and migration [22,23]. The animal Shaker superfamily comprises the so-called voltage -activated K + channel (Kv), Kv long QT, small-conductance cal- cium -activated K + channel, ... downstream events uncovered in animal cells may also have an equivalent in plant cells. Ca 2+ plays an important role as an intracellular signal in both plants and animals, includi...

Ngày tải lên: 22/03/2014, 21:21

... reasonable and testable hypothesis. This hypothesis was verified by combining glycogene microarray analysis, chromatographic glycan profiling and lectin binding [9]. Influencing N- and O-glycan galactosylation ... profiles in pancreatic adeno- carcinoma compared with normal pancreatic tissue and tissue affected by pancreatitis as detected by two-dimen- sional gel electrophoresis and mass sp...

Ngày tải lên: 29/03/2014, 21:20

... products. 7-ACA is produced from CPC by the action of D -amino-acid oxidase (step A) and a glutaryl acylase (step B). 7-ADCA is produced from penicillin G by a chemical ring expansion (step C) and the action ... glutaryl acylase and mutants Plasmids containing wild-type and desired mutated acylase genes were isolated (Plasmid Midi Kit, Qiagen Germany) and transformed to E. coli DH...

Ngày tải lên: 31/03/2014, 09:20

... for many decades, the more recent introduction of statistical methods in lan- gauge is affecting research practises in the human- ities at yet another scale. An important explana- tion for this ... for surprise content and the organisation of this dual annotation process can be improved, but in principle joining forces between NLP tech- nologies and the capacity of human annotators is a...

Ngày tải lên: 31/03/2014, 20:20

... Hole, MA, USA). We thank Andrew Gannon for help with the C. intestinalis dissection. Gene synthesis and analysis The ci0100132088 gene from the urochordate C. intestinalis is now identified in ... of AChE1 and AChE2 are from the ESTHER database [23] to distinguish the AChE described in the present study (AChE1) and another sequence proposed to be an AChE from C. intestinalis (GenBank...

Ngày tải lên: 18/02/2014, 17:20