Báo cáo khoa học: Reduction of S-nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry pptx
... Reduction of S -nitrosoglutathione by human alcohol dehydrogenase 3 is an irreversible reaction as analysed by electrospray mass spectrometry Jesper J. Hedberg*, ... conditions. Ó FEBS 20 03 GSNO reduction by human alcohol dehydrogenase 3 (Eur. J. Biochem. 270) 1251 determined by MS/MS. The MS/MS spectra of the peaks at m/z 33 7, [GSNO]H + ,...
Ngày tải lên: 31/03/2014, 07:20
... Genomics, Department of Biochemistry, University of Wisconsin- Madison, 433 Babcock Drive, Madison, WI 537 06 134 4, USA Fax: +1 608 262 37 59 Tel: +1 608 2 63 934 9 E-mail: markley@nmrfam.wisc.edu (Received ... Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25, 33 89 34 02. 23 Falk A, Ek B & Rask L (1995) Characterization...
Ngày tải lên: 18/02/2014, 14:20
... This fits their localization around the central part of glycolysis. The importance ranking matches the polar phase plane plot analysis above, and the conclusions of Madsen et al. [31 ]. This is ... system has been thoroughly characterized in terms of both biochemistry and dynamics [16– 23] , and this has led to a number of mathematical models of this system [4,24 30 ]. Our 20D mode...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Inhibitor of nuclear factor-kappaB alpha derepresses hypoxia-inducible factor-1 during moderate hypoxia by sequestering factor inhibiting hypoxia-inducible factor from hypoxia-inducible factor 1a ppt
... was knocked down, Asn8 03 hydroxylation was enhanced, and this was attenuated by FIH knockdown (Fig. 4A, right panel). On the other hand, in 0.5% oxygen, Asn8 03 was negli- gibly hydroxylated, and ... RNAs were isolated from HEK2 93 cells by Trizol reagent (Invitrogen). RNA was quantified by measuring absorbance at 260 nm. RNAs (1 lg) were reverse tran- scribed at 48 °C, and the resul...
Ngày tải lên: 29/03/2014, 23:20
Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... Q4: 3. 82% Q4: 9 .37 % Q3: 93. 40% Q3: 85.00% Q3: 90.48% Q3: 92.44% Q4: 2.89% Q4: 9.94% Q4: 3. 90% Q4: 2.81% Q1: 3. 80% Q1: 0 .34 % Q1: 0.48%Q2: 3. 76% Gate: R1 Q1: 0.54% Q2: 3. 17% Q2: 31 .49% Q1: 0. 43% Q2: ... cells Michalis D. Mantzaris 1 , Epameinondas V. Tsianos 2 and Dimitrios Galaris 1 1 Laboratory of Biological Chemistry, University of Ioannina Medical School, Greece 2 Fir...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus ppt
... cyto- plasm of Picrophilus. The kinetics of NADPH degradation was followed by measuring the rate of decrease of its absorbance at 34 0 nm over the pH range 3. 6–7.0 and at 40, 60 and 80 °C. The measured half-life ... albumin as a standard. Assay for glucose dehydrogenase activity and enzyme kinetics Glucose dehydrogenase activity was assayed spectrophoto- metrically by mea...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Expression of cholinesterases in human kidney and its variation in renal cell carcinoma types pdf
... leukae- mias and ovarian cancer [16,26] support a role for cholinesterases in carcinogenesis. This notion is given weight by the aberrant expression and structural changes of acetylcholinesterase and ... [27], the role of acetyl- cholinesterase in apoptosis [ 23] , and the downregula- tion of cholinesterases in metastasized lymph nodes [29], as well as in colorectal [30 ] and lu...
Ngày tải lên: 06/03/2014, 22:21
Báo cáo khoa học: Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor doc
... P450reductase (P450red, EC 1.6.2.4). (Received 4 November 2002, revised 23 January 20 03, accepted 10 February 20 03) Eur. J. Biochem. 270, 134 9– 135 5 (20 03) Ó FEBS 20 03 doi:10.1046/j.1 432 -1 033 .20 03. 035 08.x pheromonal ... Assessment of porcine and human 16-ene-synthase, a third activity of P450c17, in the formation of an androstenol precursor Role of recombinant cyt...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo khoa học: Binding of berberine to human telomeric quadruplex – spectroscopic, calorimetric and molecular modeling studies pot
... Biochemistry 31 , 39 47 39 55. 44 Record MT Jr, Ha JH & Fisher MA (1991) Analysis of equilibrium and kinetic measurements to determine thermodynamic origins of stability and specificity and mechanism of ... sys- tem was heated from 0 to 30 0 K at constant volume, and this was followed by equilibration for 25 ps at a constant temperature of 30 0 K and a pressure of 1 atm. Th...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Characterization of mucin-type core-1 b1-3 galactosyltransferase homologous enzymes in Drosophila melanogaster pptx
... 30 Gal(b1-O)pNP 38 44 30 37 34 Glc(a1-O)pNP 22 62 28 31 28 Man(a1-O)pNP 29 205 22 59 29 Fuc(a1-O)pNP 31 58 23 40 31 Xyl(a1-O)pNP 35 64 24 37 31 a Anti-FLAG-beads bound lysate of Sf9 cells. b The D. melanogaster b1 -3 ... diglycosyl- ated peptide MUC5AC -3 ⁄ 13 and CG 139 04-1 was more active toward MUC5AC- 13 and MUC5AC -3 ⁄ 13 Table 1. Monosaccharide acceptor specifici...
Ngày tải lên: 23/03/2014, 15:20