Báo cáo khoa học: Insights into the design of a hybrid syste

Báo cáo khoa học: Insights into the design of a hybrid system between Anabaena ferredoxin-NADP+ reductase and bovine adrenodoxin pot

... heterologous system that consists of cyanobacterial FNR and adrenal bovine Adx. Anabaena PCC 7119 FNR contains a noncovalently bound FAD group and its main physiological function is the transfer of two ... shown that ferredoxin- NADP + reductase from Anabaena and adrenodoxin from bovine adrenal glands are able to form optimal complexes for thermodynamically favoure...

Ngày tải lên : 31/03/2014, 07:20

10
400
0

Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt

... illustrates the quality of the experimental data (Fig. 2B). Reconstruction of the overall shape of the PLDa2 from the X-ray scattering data was achieved by the ab initio modeling program dammin ... plot of the data from (A) in the range 0–100 m M Ca 2+ . (C) Modiﬁed Scatchard plot of the data from (B). DA corresponds to the change in relative activity. The PLDa...

Ngày tải lên : 19/02/2014, 02:20

11
750
0

Tài liệu Báo cáo khoa học: Insights into the interaction of human arginase II with substrate and manganese ions by site-directed mutagenesis and kinetic studies Alteration of substrate speciﬁcity by replacement of Asn149 with Asp docx

... fully active state. In contrast, the arginase activity of the Asn149Asp variant was practically undetectable, both before and after the incubation with the manganese ions. Fully active His120Asn and ... 7.5, and then assayed for arginase activity in the absence of added Mn 2+ (open bars) and after full activation with the metal ion and assay in the presence of ad...

Ngày tải lên : 20/02/2014, 02:21

9
651
0

Báo cáo khoa học: Insights into the activation of brain serine racemase by the multi-PDZ domain glutamate receptor interacting protein, divalent cations and ATP ppt

... coli and the puriﬁcation of two larger fragments of GRIP and incubated the puriﬁed proteins with SR to analyse changes in activity. A hexa-His tag was introduced at the N-terminal end and a FLAG ... and mediation of neuronal migration. Proc Natl Acad Sci USA 102, 2105–2110. 13 Fujii K, Maeda K, Hikida T, Mustafa AK, Balkissoon R, Xia J, Yamada T, Ozeki Y, Kawahara R, O...

Ngày tải lên : 30/03/2014, 03:20

11
286
0

Báo cáo khoa học: Investigations into the ability of an oblique a-helical template to provide the basis for design of an antimicrobial anionic amphiphilic peptide pot

... 389–395. 43 Sospedra P, Espina M, Gomara MJ, Alsina MA, Haro I & Mestres C (2001) Study at the air ⁄ water interface of a hepatitis A N-acetylated and C-amidated synthetic peptide (AcVP3 (110–121)-NH 2 ). ... peptide characteristics and the com- position of its target membrane are important determinants of its efﬁcacy of action. Abbreviations a- AMP, a- helical antim...

Ngày tải lên : 07/03/2014, 12:20

12
688
0

Báo cáo khoa học: Insights into substrate and product trafﬁc in the Drosophila melanogaster acetylcholinesterase active site gorge by enlarging a back channel ppt

... FEBS to an allosteric interaction between PAS and CAS [8,21,25], to a lower efﬁciency of the alternative route compared to the main entrance, or to a partial overlap of the side chain of propidium and ... involving the hydroxyl of Tyr442 are indicated by a yellow dash. A B Fig. 2. View of the back channel from the active site gorge of wild- type DmAChE (A...

Ngày tải lên : 07/03/2014, 05:20

6
524
0

Tài liệu Báo cáo khoa học: Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulﬁde bond formation and their contribution to venom complexity pdf

... (1994) Structural interaction of natural and synthetic inhibitors with the venom metalloproteinase atrolysin C (form d). Proc Natl Acad Sci USA 91, 8447–8451. 20 Kumasaka T, Yamamoto M, Moriyama H, Tanaka ... FEBS 24 Watanabe L, Shannon JD, Valente RH, Rucavado A, Alape-Giron A, Kamiguti AS, Theakston RD, Fox JW, Gutierrez JM & Arni RK (2003) Amino acid sequence and crystal str...

Ngày tải lên : 18/02/2014, 18:20

15
476
0

Tài liệu Báo cáo khoa học: nsights into the reaction mechanism of glycosyl hydrolase family 49 Site-directed mutagenesis and substrate preference of isopullulanase doc

... CTGACCTtcTGGCATAAC ACCGGtGAAATC AgeI W32F CCTGGTtcCATAAC ACCGGtGAAATC AgeI W240F GGTGCT gAGCTCAAGTGTGACTTtcGTCTAC SacI W402F CCGGTG GTcGAcTTTGGTTtcACGCCC SalI E273Q ACGTACTGCTgTCCGGAA AGtACtCCATGGCCGC ScaI D353N ... ACGTACTGCTgTCCGGAA AGtACtCCATGGCCGC ScaI D353N TCCAATCCGTtAGTC TGgCCaTAGAACGCGC MscI E356Q GGAGAAT GGTgCCAGGGTACATTTcCAATCCGTCA KpnI D372N AATACAT CTTaAGGCCGTCGTtGTCGGTGTGG A II...

Ngày tải lên : 19/02/2014, 16:20

8
551
0

Tài liệu Báo cáo khoa học: "Insights into Non-projectivity in Hindi" docx

... us__lekhakiiy__asmitaa__ko ham sagarv prakaashak__ke−saamane rakhakar baat__karate_ _the b) a) _ROOT_ isake__baad vah jamaan__shaah aur−phir 1795__meM shaah__shujaa ko milaa this after it Jaman Shah and then ... then socialism was its solid base b) _ROOT_ gorkii yadi is__naye__saahity__ke__srishtikartaa the to samaajavaad isakaa Thos aadhaar thaa a) _ROOT_ yadi rupayoM kii zaruurat t...

Ngày tải lên : 20/02/2014, 09:20

8
506
0

Tài liệu Báo cáo Y học: Insights into the reaction mechanism of Escherichia coli agmatinase by site-directed mutagenesis and molecular modelling ppt

... 5¢-GCCCACACC AAT ACCTATGCG-3¢) and the 3¢ complementary primer 5¢-AT TAATGGCATGCTTTACCCGT-3¢.UsingthePCR products of agmatinase with the GﬁAandCﬁT substi- tutions in the coding and noncoding strands, ... Scheme of the binuclear manganese cluster and the localization of the side-chains of Asp153 and Asn153 in the modelled structures of wild-type and D153N muta...

Ngày tải lên : 21/02/2014, 01:21

5
475
0