Báo cáo khoa học: The heterogeneity of mast cell tryptase from human lung and skin Differences in size, charge and substrate affinity ppt

... differing values of K m and k cat . Fig. 9. pH profile of human skin and lung tryptase in the presence and absence of heparin. (j) skin tryptase, no heparin (h) skin tryptase + 100 lgÆmL )1 heparin ... (A–C) mast cells purified from skin tissue (SMC1, 6 and 10), and (D) a preparation of purified skin tryptase (ST2). Fig. 3. Lectin binding to lung...

Ngày tải lên: 31/03/2014, 07:20

... regulating the cGMP affinity of the PDE11A4 tandem GAF domain and thus allosterically affect PDE11 activity. The role of the N-terminus of the PDE11A4 GAF tandem domain The N-termini that precede the ... the tandem GAF domain of PDE10, and by cGMP when linked to the tandem GAF domain of PDE2, )5or)11. The bio- chemical properties of signalling by human t...

Ngày tải lên: 16/03/2014, 06:20

... Gly144, and the amide group of Gly143 is hydrogen-bonded to the distal ligand of the heme iron (the residue numbers of the three glycines are the same in rat HO-1 and in human HO-1). The Gly139–Gly143 ... solution. Conformation of the distal helix As in the previously reported s tructures of o ther heme– HOs, the heme of heme–Syn HO-1 is sandwiched b...

Ngày tải lên: 23/03/2014, 13:20

... of differing lines in contrast with others such as Met20, which remain single peaks. Interestingly, these line-broadend residues are located around the retinal indicating heterogeneity in this region. ... important differences between the bR- and O-like states [17]. The most notable differ- ences are in the extracellular half of the protein and in the loop regi...

Ngày tải lên: 23/03/2014, 13:20

... types (Hc1 and Hc2) with the exception of PmaHc1. An insertion of nine amino acids in PamHc2 starting at amino acid 435 is unique to subunit types 2. On the amino acid level, the hemo- cyanin subunits ... means of two Cu + ions, each of which is coordinated by three histidines in two distinct binding sites. Some hemocyanins assemble into large oligomers of up to 8 · 6 su...

Ngày tải lên: 18/02/2014, 08:20

... no in uence of the additive on the chaperone activity of aB-crystallin (i.e. the effect of the additive on the aggregation of the target protein is inde- pendent and different from that of the ... thought to be determined by a number of fac- tors, including the rate of unfolding, the amino acid sequence of the protein, the experimental conditions an...

Ngày tải lên: 18/02/2014, 16:20

... nucleosomes in the main peak, indicates that crosslinking is almost complete in the middle of the fibre, but some of the end-nucleo- somes are not crosslinked to the rest and therefore must originate from ... trinucleosomes in the products. The mononucleosomes evidently come from the ends of the fibre because of the corresponding decrease of the avera...

Ngày tải lên: 18/02/2014, 18:20

... binding affinity was observed in the case of binding of the M.HhaI with the X + CG ⁄ CGM duplex containing the (+)-cis-B[a]P- N 2 -dG adduct on the 5¢-side of the target dC residue. The binding of ... CGM and G CX + ⁄ CGM), 5-methylcytosine was introduced into one of the strands of the recognition site instead of the target cytosine. The melting curves...

Ngày tải lên: 19/02/2014, 00:20

... proteins involved in the trafficking of the receptor, including c-Cbl, could account for its intracellular retention. By contrast, TDI mutations in the ECD or disruption of the termination codon induced ... mutations affecting the receptor in at least two different ways. Conversion of Lys650 into methionine in the TK2 domain induces hyperphosphorylation and marked i...

Ngày tải lên: 19/02/2014, 00:20

... disrupted, and this gives rise to an increase in K m . Also, the polarity is changed, and the increase in the size of the side chain may create steric hindrance for the substrate, making the base ... transition state and holding E52 in position during catalysis; and (b) investigate the amino acid residues involved in sub- strate binding (Y70 and Q81). The...

Ngày tải lên: 19/02/2014, 02:20