Báo cáo khoa học: Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? ppt

... pyruvate ratio is a very important factor for the determination of the degree of phosphorylation of EIIA as the PTS reaction network works in a reversible manner. Therefore, in our repre- sentation, ... sites for every transcription factor; this number varies for every gene. For example, Nishio et al. [15] take into account that the mlc gene possesses two binding sites for Crp and two for Mlc...

Ngày tải lên: 18/02/2014, 13:20

... expression and purification E. coli Pta and Pta-F1, Pta-F2 and Pta-F3 were produced in E. coli K-12 AG1, E. coli BL21(DE3) or E. coli M15 containing the corresponding expression vectors (p–Pta, pET28–F1, ... mm PEP or 10 mm pyruvate (Fig. 5B). On the other hand, E. coli Pta acetyl phosphate- forming activity was measured in the presence of acti- vators (pyruvate or PEP) and i...

Ngày tải lên: 06/03/2014, 11:20

... proteins are responsible for EPEC entry into host cells. Monolayers were infected with EPEC for the indicated times and stained for ZO-1 (red) and bacteria (green) to assess bacterial entry. Yellow areas ... 2008 The Authors Journal compilation ª 2008 FEBS and reoviruses are known to initiate infection via TJs [10]. Microorganisms utilize lipid rafts in order to exert their effects on ho...

Ngày tải lên: 16/03/2014, 04:20

... 0.34 III ProRS e – II LysRS f – III a Value for Saccharomyces carlsbergensis, no data available for E. coli; b value for Lupinus luteus and bovine, no data available for E. coli; c value for Paracoccus ... the k cI repressor, which may repress transcription by E. coli RNAP at 30 °C. The optimized concentrations of T7 or E. coli RNAP used in the present work were found to be suitable...

Ngày tải lên: 16/03/2014, 18:20

... host cells. Abbreviations ANS, 8-anilinonaphthalene-1-sulfonate; EHEC, enterohaemorrhagic Escherichia coli; EPEC, enteropathogenic Escherichia coli; HSQC, heteronuclear single quantum correlation; ... form a conduit for translocation of bacterial effector proteins into host cells [23]. It has been suggested that EspA filaments attach to host cells via EspB ⁄ D pore complexes and th...

Ngày tải lên: 16/03/2014, 18:20

... purification Wild-type and mutant forms of recombinant E. coli CTPS were expressed in and purified from E. coli strain BL21(DE3) cells transformed with either mutant or wild- type plasmid pET15b-CTPS1 ... posi- tions of the mismatches are underlined. Potential mutant plasmids were isolated and used to transform competent DH5a cells. These cells were used for plasmid maintenance and...

Ngày tải lên: 23/03/2014, 17:22

... LT-I receptors in parental HT29 cells and that these non-GM1 receptors may account for  50% of the cyclic AMP response elicited by the toxin in these cells [16]. The results from this work indicate ... Comparison of the glycolipid-binding specificities of cholera toxin and porcine Escherichia coli heat-labile enterotoxin: identification of a receptor-active non-gang- lioside glycolipid...

Ngày tải lên: 30/03/2014, 10:20

... Are UV-induced nonculturable Escherichia coli K-12 cells alive or dead? Andrea Villarino 1,2 , Marie-Noe¨ lle Rager 3 , Patrick A. ... (Sigma). Cells that exceeded at least twice the mean length of cells before DVC were scored as elongated. The proportion of DVC positive cells was corrected by the proportion of elongated cells ... of nonculturable cells is often...

Ngày tải lên: 31/03/2014, 01:20

... different perspectives and one newswire cor- pus that covers various topics, as summarized in Table 1. No stemming algorithms is performed; no stopwords are removed. Corpus Subset |D| ¯ |d| V bitterlemons Palestinian ... selected for discus- sion (e.g., “Disengagement: unilateral or coordi- nated?”), and a Palestinian editor and an Israeli editor each contribute one article addressing the...

Ngày tải lên: 23/03/2014, 18:20

... differ- ent metal or redox states are shown. Cysteine residues involved in copper binding or disulfide bond formation are shown as yellow sticks. Histidine Cu(I) ligands in Sco1 and Sco2 are shown as ... transporter or two uni- directional transporters [75]. Once copper is stored in the matrix, integral membrane proteins with soluble domains that localize to both the matrix and the...

Ngày tải lên: 14/02/2014, 18:20