Báo cáo khoa học: Selection of peptides inhibiting a b-lactamase-like activity docx

... forward primer AB 348, 5¢-TTAGCAAAACCTC ATACAGAA-3¢, and the backward primer AB 349, 5¢-GATGCTGTCTTTCGCTGCTGAG-3¢,wereusedfor DNA amplification. The M13 forward primer, 5¢-ATTCACCTCGAAAGCAAGCTG-3¢,wasusedfor sequencing. Table ... Designing new antibiotic molecules is a flourishing activity of medical research nowadays. The in vitro selection of peptides able to inhibit b-lactamase...

Ngày tải lên: 31/03/2014, 01:20

... MINIREVIEW Selection of stably folded proteins by phage-display with proteolysis Yawen Bai and Hanqiao Feng Laboratory of Biochemistry, National Cancer Institute, Bethesda, MD, USA To facilitate the ... several rounds of protease digestion and amplification of the library, variants with high thermodynamic stability were enriched over those that have low thermodynamic stability. A s...

Ngày tải lên: 19/02/2014, 12:20

... NaOH and 1 m NaCl. The data were ana- lyzed using biaevaluation software with appropriate sub- traction methods, and the bivalent analyte model was used to account for avidity effects associated ... ranging from the low-nanmolar to mid-nanomolar range. This spectrum of affinities emphasizes the advantages of employing bivalent IgG display, as it facilitates the selection of both modera...

Ngày tải lên: 22/03/2014, 21:21

... Tamamura H, Imai M, Ishihara T, Masuda M, Funakoshi H, Oyake H, Murakami T, Arakaki R, Nakashima H, Otaka A et al. (1998) Pharmacophore identification of a chemokine receptor (CXCR4) antag- onist, ... isolated at the highest frequency acted as an antagonist (IC 50 =23lm) and displayed an affinity of 5.6 lm. A discrepancy in the potency of clone 3 in phage format (nanomolar range ac...

Ngày tải lên: 28/03/2014, 22:20

... Methyl a- d-glucopyranoside was used as a glucose analog that is not a substrate of hexokinase in a glucose assay system. The data were analyzed on the basis of the Michaelis–Menten equation; the maximum activity ... enzymatic activity of the acceptor reaction for the nigerooligo- saccharide with a degree of polymerization of 2–6 and methyl a- D-gluco- pyranoside as...

Ngày tải lên: 14/02/2014, 22:20

... Pharmacology, University of Calgary, Alberta, Canada 2 School of Biomedical Sciences, University of Newcastle, Callaghan, NSW, Australia Long-range signaling Biological organs display coordinated ... membrane depolarization activating or advancing the phase of other Ca 2+ stores. The electrical and chemical transduction pathways are as depicted in Fig. 3. The key mechanisms are as fol...

Ngày tải lên: 16/02/2014, 09:20

... MINIREVIEW Synchronization of Ca 2+ oscillations: a capacitative (AC) electrical coupling model in neuroepithelium Masayuki Yamashita Department of Physiology I, Nara Medical University, Kashihara, Japan Structural ... packed in the basal layer (Fig. 1C). The voltage fluctuations of the Ca 2+ store will induce ACs, which can pass the series capaci- tance of the ONM and the plasma mem...

Ngày tải lên: 16/02/2014, 09:20

... presence of ATP, with concomitant generation of ETA and ETA -A fragments. Incubation in the absence of ATP revealed a small amount of degrada- tion for intact ETA, whereas no degradation was observed ... postinjection) AB Fig. 1. Kinetics of appearance of ETA in hepatic plasma membranes and endosomes after toxin administration. Rat hepatic plasma mem- brane (A) and endosomal fr...

Ngày tải lên: 18/02/2014, 04:20

... 2. Steady-state signalling characteristics of a covalent-modification cycle for equal catalytic activity of kinase and phosphatase (A C), for 10-fold higher catalytic activity of the kinase (D–F), ... fraction of the target is no longer accessible to other kinases and phosphatases. Available data about phosphatase concentrations sug- gest that they are also likely to be of the...

Ngày tải lên: 19/02/2014, 07:20

... polymerase a- primase complex during meiosis in Coprinus cinereus Satoshi Namekawa, Fumika Hamada, Tomoyuki Sawado†, Satomi Ishii, Takayuki Nara‡, Takashi Ishizaki, Takashi Ohuchi, Takao Arai and ... Hatanaka, M., Kimura, S., Oshige, M., Tsuya, Y., Mizushina, Y., Sawado, T., Aoyagi, N., Matsumoto, T., Hashi- moto, J. & Sakaguchi, K. (1998) Purification and characterization of a 100 kD...

Ngày tải lên: 20/02/2014, 11:20