Báo cáo khoa học: ˚ Crystal structure at 3 A of mistletoe le

Báo cáo khoa học: ˚ Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose pdf

... identically conserved asparagine (Asn47, Asn256), which donates a hydrogen bond to 3- OH of galactose, also donates a hydrogen bond to the main- chain O of valine (Val24, Val 236 ), while the main-chain ... structure at 3 A ˚ of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose Hideaki Niwa 1 , Alexander...

Ngày tải lên : 31/03/2014, 01:20

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Báo cáo khoa học: Crystal structure and enzymatic properties of a bacterial family 19 chitinase reveal differences from plant enzymes pdf

... chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six subsites [26 ,30 ]. However, the catalytic domains of ChiG, ChiC and most ... Research, Inc., Waltham, MA, USA). The ampliﬁcation protocol consisted of an initial denaturation cycle of 30 s at 98 °C, followed by 30 cycles of 10 s at 98 °C, 30 s at...

Ngày tải lên : 07/03/2014, 11:20

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Báo cáo khoa học: Crystal structure and solution characterization of the activation domain of human methionine synthase doc

... Sequencing of the resulting vector, pACT, revealed two missense mutations that resulted in conversion of an Asp at position 9 63 to a Glu and a Lys to an Arg at position at 1071. (At this stage we cannot ... hydrated molecular mass of hMS activation domain was determined by MALLS coupled to gel-ﬁltra- tion chromatography. Approximately 50 lg of both wild- type and mutant...

Ngày tải lên : 23/03/2014, 09:21

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Tài liệu Báo cáo khoa học: Crystal structure of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-D-glyceric acid Selective inhibition by structure-based design docx

... Oliva, G., Castellano, E.E., Garratt, R.C. & Craivich, A. (1998) Set-up and experimental parameters of the protein crystallography beamline at the Brazilian National Synchrotron Laboratory. ... Processing of X-ray diﬀrac- tion data collected in oscillation mode. Methods Enzymol. 276, 30 7 32 6. 38 . Navaza, J. (1994) AMoRe: an automated package for molecular replacement. Acta Cry...

Ngày tải lên : 20/02/2014, 02:21

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Tài liệu Báo cáo khoa học: Crystal structure of thiamindiphosphate-dependent indolepyruvate decarboxylase from Enterobacter cloacae, an enzyme involved in the biosynthesis of the plant hormone indole-3-acetic acid doc

... calculation of low resolution models of E. cloacae PDC (EcPDC) from small angle X-ray solution scattering data [21] had indicated that the quaternary structure of IPDC is more similar to that of ... interface is with 34 14 A ˚ 2 comparable to that of pyruvamide-activated ScPDC [29]. In ZmPDC, the interaction area is larger ( 438 7 A ˚ 2 ),whereasitissmallerin Fig....

Ngày tải lên : 20/02/2014, 11:20

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Báo cáo khoa học: Crystal structure of salt-tolerant glutaminase from Micrococcus luteus K-3 in the presence and absence of its product L-glutamate and its activator Tris pdf

... 747 32 Chantawannakul P, Yoshimune K, Shirakihara Y, Shiratori A, Wakayama M & Moriguchi M (20 03) Crys- tallization and preliminary X-ray crystallographic stud- ies of salt-tolerant glutaminase ... Protein Crystallography, No.26 SERC Daresbury Laboratory, Warrington, UK. 34 Navaza J (1994) AMoRe: an automated package for molecular replacement. Acta Crystallogr A 50, 157–1 63. 35...

Ngày tải lên : 06/03/2014, 09:22

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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

... concentrations. Database Structural data are available in the Protein Data Bank database under the accession numbers 3PPS and 2VDZ Structured digital abstract l laccase binds to laccase by x-ray crystallography ... Journal compilation ª 2011 FEBS 2295 Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases Juha P. Kallio...

Ngày tải lên : 14/02/2014, 18:20

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Tài liệu Báo cáo khoa học: Crystal structure of importin-a bound to a peptide bearing the nuclear localisation signal from chloride intracellular channel protein 4 ppt

... importin -a nuclear import pathway, provided that CLIC4 can undergo a conformational rearrangement that exposes the NLS in an extended conformation. Database Structural data are available in ... the mean B factor (excluding water molecules and peptide atoms) and r j is the standard deviation of B factors. The normalized B factors have a zero mean and unit variance. All atoms that sat...

Ngày tải lên : 14/02/2014, 19:20

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Tài liệu Báo cáo khoa học: Crystal structure of the cambialistic superoxide dismutase from Aeropyrum pernix K1 – insights into the enzyme mechanism and stability pdf

... tyrosine 34 to structure and catalysis. Biochemistry 48, 34 17 34 24. 31 Nakamura T, Matsumura H, Inoue T, Kai Y, Uegaki K, Hagihara Y, Ataka M & Ishikawa K (2005) Crystallization and preliminary ... hyper-thermophilic crenarchaeon, Aeropyrum pernix K1. DNA Res 6, 83 101. 11 Nakamura T, Yamamoto T, Inoue T, Matsumura H, Kobayashi A, Hagihara Y, Uegaki K, Ataka M, Kai Y & Ishikaw...

Ngày tải lên : 14/02/2014, 22:20

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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc

... substrate-free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚ . Distinct conformational changes ... phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two...

Ngày tải lên : 16/02/2014, 09:20

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