Báo cáo khoa học: ˚ Crystal structure at 3 A of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose pdf

... identically conserved asparagine (Asn47, Asn256), which donates a hydrogen bond to 3- OH of galactose, also donates a hydrogen bond to the main- chain O of valine (Val24, Val 236 ), while the main-chain ... structure at 3 A ˚ of mistletoe lectin I, a dimeric type-II ribosome-inactivating protein, complexed with galactose Hideaki Niwa 1 , Alexander...

Ngày tải lên: 31/03/2014, 01:20

... chitinases have catalytic domains that are at least as large as those of the plant enzymes and that may contain at least six subsites [26 ,30 ]. However, the catalytic domains of ChiG, ChiC and most ... Research, Inc., Waltham, MA, USA). The amplification protocol consisted of an initial denaturation cycle of 30 s at 98 °C, followed by 30 cycles of 10 s at 98 °C, 30 s at...

Ngày tải lên: 07/03/2014, 11:20

... Sequencing of the resulting vector, pACT, revealed two missense mutations that resulted in conversion of an Asp at position 9 63 to a Glu and a Lys to an Arg at posi- tion at 1071. (At this stage we cannot ... hydrated molecular mass of hMS activation domain was determined by MALLS coupled to gel-filtra- tion chromatography. Approximately 50 lg of both wild- type and mutant...

Ngày tải lên: 23/03/2014, 09:21

... Oliva, G., Castellano, E.E., Garratt, R.C. & Craivich, A. (1998) Set-up and experimental parameters of the protein crystallography beamline at the Brazilian National Synchrotron Laboratory. ... Processing of X-ray diffrac- tion data collected in oscillation mode. Methods Enzymol. 276, 30 7 32 6. 38 . Navaza, J. (1994) AMoRe: an automated package for molecular replacement. Acta Cry...

Ngày tải lên: 20/02/2014, 02:21

... calculation of low resolution models of E. cloacae PDC (EcPDC) from small angle X-ray solution scattering data [21] had indicated that the quaternary structure of IPDC is more similar to that of ... interface is with 34 14 A ˚ 2 comparable to that of pyruvamide-activated ScPDC [29]. In ZmPDC, the interaction area is larger ( 438 7 A ˚ 2 ),whereasitissmallerin Fig....

Ngày tải lên: 20/02/2014, 11:20

... 747 32 Chantawannakul P, Yoshimune K, Shirakihara Y, Shiratori A, Wakayama M & Moriguchi M (20 03) Crys- tallization and preliminary X-ray crystallographic stud- ies of salt-tolerant glutaminase ... Protein Crystallography, No.26 SERC Daresbury Laboratory, Warrington, UK. 34 Navaza J (1994) AMoRe: an automated package for molecular replacement. Acta Crystallogr A 50, 157–1 63. 35...

Ngày tải lên: 06/03/2014, 09:22

... concentrations. Database Structural data are available in the Protein Data Bank database under the accession numbers 3PPS and 2VDZ Structured digital abstract l laccase binds to laccase by x-ray crystallography ... Journal compilation ª 2011 FEBS 2295 Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases Juha P. Kallio...

Ngày tải lên: 14/02/2014, 18:20

... importin -a nuclear import pathway, provided that CLIC4 can undergo a conforma- tional rearrangement that exposes the NLS in an extended conformation. Database Structural data are available in ... the mean B factor (excluding water molecules and peptide atoms) and r j is the standard deviation of B factors. The normalized B factors have a zero mean and unit variance. All atoms that sat...

Ngày tải lên: 14/02/2014, 19:20

... tyrosine 34 to structure and catalysis. Biochemistry 48, 34 17 34 24. 31 Nakamura T, Matsumura H, Inoue T, Kai Y, Uegaki K, Hagihara Y, Ataka M & Ishikawa K (2005) Crystallization and preliminary ... hyper-thermophilic crenarchaeon, Aeropyrum pernix K1. DNA Res 6, 83 101. 11 Nakamura T, Yamamoto T, Inoue T, Matsumura H, Kobayashi A, Hagihara Y, Uegaki K, Ataka M, Kai Y & Ishikaw...

Ngày tải lên: 14/02/2014, 22:20

... substrate-free AppA the C a atoms are 2.41 A ˚ apart, whereas for the substrate-free PhyK and the substrate-loaded AppA the averaged distance is only 1.87 A ˚ . Distinct conformational changes ... phytate with pH optima in the acidic range. They consist of two domains, a large a ⁄ b domain and a small a domain with the catalytic site at the interface of the two...

Ngày tải lên: 16/02/2014, 09:20