Báo cáo khoa học: The histidine-phosphocarrier protein of Streptomyces coelicolor folds by a partially folded species at low pH ppt

... as a two-state process without the accumulation of intermediates at neutral and moderately basic conditions (pH 7–9) at 25 °C. The stability of the protein, at pH 7.5 and 25 °C, as obtained by chemical ... phosphoenolpyruvate phosphotransferase system (PTS) catalyzes the uptake and phosphorylation of carbo- hydrates in most bacterial species, via a cascade of...

Ngày tải lên: 31/03/2014, 01:20

... as a part of Gag, as an unassembled protein, and as an independent protein forming the mature capsid. Remarkably, the CA poly- peptide appears to have evolved an extraordinary con- formational ... mediated by water molecules, and a substantial hydrophobic central area is absent. All these features differentiate the NTD– NTD interface from the CTD dimerization interface and...

Ngày tải lên: 18/02/2014, 06:20

... quantitative value of the affinity constant of the CAC1–CTD complex was determined by fluorescence (using an anthraniloyl-labeled peptide), affinity chro- matography and isothermal titration calorimetry. ... the assembly-competent mutants E18 7A and N18 3A: the conformations of the two assembly-incompetent mutants (Y16 9A and L21 1A) in the absence of CAI are the same a...

Ngày tải lên: 18/02/2014, 06:20

... incorpo- rated into virions and their ability to undergo aminoa- cylation [11]. Because the aminoacylation defect of these tRNA variants was primarily in the K m parame- ter, it was suggested that binding ... viral infectivity makes it a potential therapeutic target. CyPA is shaped like a b-barrel formed by eight anti- parallel beta strands with two alpha helices that cap the to...

Ngày tải lên: 07/03/2014, 00:20

... exhib- ited acid phosphatase activity at almost the same level as the C-terminal domain alone. These results indicate that RNase H and acid phosphatase activities of the full length SCO2299 protein ... fructose-2,6-bisphosphatase or other acid phosphatases. This suggests that its function might not be the same as the CobC generating a- ribazole from a- ribazole-5¢-phosphate...

Ngày tải lên: 19/02/2014, 18:20

... been at- tacked by statistical methods. Because probabilistic approaches solve PP at- tachment as a natural subtask of parsing anyhow, the obvious application of a PP attacher is to in- tegrate ... syntax analysis, the di- chotomy manifests itself in the existence of learnt and handwritten grammars of natural languages. A great many formalisms have been advanced that...

Ngày tải lên: 17/03/2014, 04:20

... )1 and +1, is incorporated into the carbonium cation intermediate. Tyr374 in the substrate binding state catches a water molecule at a distance of 2.6 A ˚ ,andthiswaterisalso captured by two catalytic ... of Kunkel et al. [17]. To construct the Y37 4A mutant, the following oligonucleotide was used as a mutagenic primer: 5¢-GATCACACTCTCGCG AAACA AATAATTCATCACCG-3¢. Th...

Ngày tải lên: 23/03/2014, 12:20

... Oita, Japan Many natural catalytic antibodies have been discov- ered in the last decade. The first natural catalytic anti- body was isolated from the serum of an asthma patient [1], and this antibody ... FEBS because of its low concentration. At 4 h of incubation (lane 2), significant changes of the band pattern were observed. The bands of partially dissociated urease...

Ngày tải lên: 07/03/2014, 21:20

... phosphorylated (lane 3). After boiling, IIA Crr -phosphate became dephosphorylated indicating a heat-labile aminoacyl phosphorylation of IIA Crr (lane 5) as occurs by histidine phosphorylation. When ... glutamate. Is IIA Crr phosphorylated by HPr? In vitro phosphorylation assays were performed to demon- strate phosphoenolpyruvate-dependent phosphorylation of IIA Crr in the presenc...

Ngày tải lên: 17/03/2014, 23:20

... artifi- cial substrate to assess the kinase activity and GST alone was used as a negative control. The top panel shows the kinase assay visualized by autoradiography and the bottom panel shows the ... Yasuda T (2002) Activation of AtMEK1, an Ara- bidopsis mitogen-activated protein kinase kinase, in vitro and in vivo: analysis of active mutants expressed in E. coli and gener...

Ngày tải lên: 14/02/2014, 19:20