Báo cáo khoa học: The oxygenase component of phenol hydroxylase from Acinetobacter radioresistens S13 docx

... 629–633. Ó FEBS 2003 A. radioresistens S13 phenol hydroxylase (Eur. J. Biochem. 270) 2253 The oxygenase component of phenol hydroxylase from Acinetobacter radioresistens S13 Sara Divari 1 , Francesca ... wastes, from which the strain was isolated by our group [27]. This paper describes the purification, the characterization and the catalytic properties of...

Ngày tải lên: 31/03/2014, 01:20

... considered to be the branch of engineering that uses computers to do useful things with linguistic signals, but it can also be viewed as an extended test of computational theories of human cognition; ... identify the context-appropriate sense. A statisti- cal approach generally involves the use of word co-occurrence statistics to create a semantic hyperspace where each word,...

Ngày tải lên: 20/02/2014, 18:20

... structure of Anx(Gh1) from cotton emphasizes the high conservation of the unique annexin fold even among the members of the plant subfamily of annexin proteins. The fold is comprised of the arrangement ... either distorted or the access of a cation to the site is blocked by the p resence of a side chain of a b asic residue. I n case of the IIAB s ite (F...

Ngày tải lên: 17/03/2014, 03:20

... feature of the mechanism is the devel- opment of a strong hydrogen bond between the incoming nucleophile and the leaving group in the transition state of the reaction. The phosphate site of Schizophyllum ... How- ever, even in the absence of correction for the influence of molecular volume and hydrophobicity of the rescue reagent, Table 2 shows clearly that t...

Ngày tải lên: 30/03/2014, 04:20

... diphosphate (ThDP) is the deprotonation of the C2 atom of the thiazolium ring (marked by an asterisk). The resulting ylid of ThDP (I) can attack the carbon atom of the carbonyl group of the substrate ... omitted in the final model, and the position of the residues adjacent to the loop are labeled. The orientation of the sub- unit is the same as that o...

Ngày tải lên: 30/03/2014, 10:20

... paper reports the isolation and characterization of the regulatory moiety of the multicomponent enzyme phenol hydroxylase from Acinetobacter radioresistens S13 grown on phenol as the only carbon ... alkene monooxygenase from Nocardia corallina [8]. In phenol hydroxylase of A. radioresistens S13, the third component is needed for the overall enzyme ac...

Ngày tải lên: 21/02/2014, 00:20

... characterized the contribution of these assay components by omitting them individually. In the absence of ferredoxin the reaction rate decreased to 46.5% of the complete reaction while the absence of ascorbate ... that the residue numbers in the figure do not correspond exactly to those in the table because of the absence of the chloroplast transit peptide seque...

Ngày tải lên: 30/03/2014, 11:20

... intravenous lines). The observers were not involved in the daily care of the patients, and ICU physicians were not aware of this part of the observation. As a consequence, the clinical relevance of the predefined ... for other infections, and the absence of radiological signs of pulmonary congestion might have resulted in another fluid therapy regimen. We did not sco...

Ngày tải lên: 25/10/2012, 10:39

... together with the E2F site [20,21]. The DRS ⁄ CHR in the B-Myb pro- moter deviates most from other CHR sequences with its two-nucleotide exchange from the CHR consensus (Fig. 2). Changing the ... turns of the DNA double helix yields binding of the two or three NF-Y complexes on the same side of the DNA. Conservation of spacing is required for optimal pro- moter a...

Ngày tải lên: 16/02/2014, 09:20

... helix 4 of the NTD of a neighboring subunit in the hexamer. Additional contacts involve helix 11 of the CTD and the C-terminal end of helix 7 of the NTD of the neigh- boring subunit. The NTD–CTD ... of the precise residues and interac- tions involved in the NTD–CTD interface [46]. Most of these involve side chains from helix 8 of the CTD which pack agai...

Ngày tải lên: 18/02/2014, 06:20