Báo cáo khoa học: Accessory proteins functioning selectively and pleiotropically in the biosynthesis of [NiFe] hydrogenases in Thiocapsa roseopersicina docx

... accessory proteins and assembly (Eur. J. Biochem. 270) 2227 Accessory proteins functioning selectively and pleiotropically in the biosynthesis of [NiFe] hydrogenases in Thiocapsa roseopersicina Gergely ... protein, likely to be involved in the processing of the HupL subunit [15]. It is plausible to assume that HynD is involved in the maturatio...

Ngày tải lên: 31/03/2014, 01:20

... staining of P-glycoprotein Cytospins of GLC4 and GLC4 ⁄ P-glycoprotein cells were incubated for 1 min with hematoxylin for staining of nuclei. To assess the localization of P-glycoprotein expres- sion ... Center Groningen, University of Groningen, the Netherlands 3 Department of Medical Oncology, University Medical Center Groningen, University of Groningen, the Netherlan...

Ngày tải lên: 30/03/2014, 08:20

... attack of the L-hOrn 3 a-amino group onto l-haOrn 4 . Taking into account that the synthetases involved in the bio- synthesis of the DKP-inheriting toxins thaxtomin and fumitremorgin also contain ... pathogens, the interest in the structural and functional characterization of these compounds is growing and may result in the synthesis of specific inhibitors bas...

Ngày tải lên: 16/02/2014, 09:20

... by the second gene in the las operon (Fig. 1). In order to obtain strains with increased PK activity an additional copy of the pyk gene was recombined into the TP901-1 attachment site using the ... to result in equal amounts of formate and acetyl-CoA and the resulting acetyl-CoA is then metabolized into equal amount of ethanol and acetate to maintain the redox...

Ngày tải lên: 19/02/2014, 17:20

... 3.1.4 Generating distance feature. In the final stage of preparing training data, we add the distance, i.e. number of words, between a Chinese token feature and the English term in question, ... training and testing. We obtained a total of 155,310 article pairs, from which we then randomly selected 13,150 and 2,181 titles as seeds to obtain the training and te...

Ngày tải lên: 19/02/2014, 19:20

... preparation. Of particular importance is the finding that exogenous cADPR failed to increase the spontaneous release of ATP in the absence of CD38. In other words, the presence of CD38 is mandatory for the ... suggesting that the two adenine nucleotides may work in synergy to maintain the resting tone of the bladder. In conclusion, extracellular cADPR enhances...

Ngày tải lên: 05/03/2014, 23:20

... biosynthesis of colla- gen and other hydroxyproline ⁄ hydroxylysine-containing proteins ⁄ peptides; synthesis of secondary metabolites, hormones and cytokines [1]; oxidative protein folding and endoplasmic ... Escherichia coli. The recombinant protein contained an engineered enterokinase cleavage site in the junction between the GST tag and the Rv1771 sequence. Upon...

Ngày tải lên: 07/03/2014, 12:20

... 14 Da mass increase consistent with methylation of the side chain carboxyl group of the N-terminal Asp residue confirm- ing amidation of the C-terminus. Partial methylation of the internal peptide ... Gla(3)–TxVI: confirmation of the presence of BrTrp. Product ion mass spectrum of the doubly charged ion at m ⁄ z ¼ 660.18. The isotopic distribution of the b 2 ion...

Ngày tải lên: 23/03/2014, 11:20

... methods. These approaches offer the opportunity to probe the basic mechanism of protein–protein interactions in great detail. First, the affinity of the LAT-binding proteins, PLC-c1, Grb2 and Gads, ... for the lack of in vivo binding of Gads to phosphorylated LAT tyrosine 132) and the formation of multiprotein signaling complexes (the reason for the lack of...

Ngày tải lên: 23/03/2014, 15:21

... m M dithiothreitol, in a final volume of % 350 lL, and by incubating the resulting solutions for 1 h at 37 °C in the dark. After reduction, 1 lLof4-vinylpyri- dine was added, and the incubation was continued ... bradykinins, caeruleins, bombesins, sauv- agine, opioid peptides (dermorphins and deltorphins), antimicrobial peptides (dermaseptins and adenoregulin), and trypto...

Ngày tải lên: 30/03/2014, 13:20