Báo cáo khoa học: Accessory proteins functioning selectively and pleiotropically in the biosynthesis of [NiFe] hydrogenases in Thiocapsa roseopersicina docx

... accessory proteins and assembly (Eur. J. Biochem. 270) 2227 Accessory proteins functioning selectively and pleiotropically in the biosynthesis of [NiFe] hydrogenases in Thiocapsa roseopersicina Gergely ... protein, likely to be involved in the processing of the HupL subunit [15]. It is plausible toassume that HynD is involved in the maturation of the HynL protein. Indeed, in the strain harboring the ... T. roseopersicina and to understand their physio-logical roles. The determination of the speciﬁcity of the accessory proteins is a challenging exercise in this micro-organism because of the...

Báo cáo khoa học: Cyclosporin A-induced oxidative stress is not the consequence of an increase in mitochondrial membrane potential doc

... staining of P-glycoproteinCytospins of GLC4 and GLC4 ⁄ P-glycoprotein cells wereincubated for 1 min with hematoxylin for staining of nuclei. To assess the localization of P-glycoprotein expres-sion ... Center Groningen, University of Groningen, the Netherlands3 Department of Medical Oncology, University Medical Center Groningen, University of Groningen, the NetherlandsKeywordscyclosporin A; mitochondria; ... that neithermitochondria, inhibition of calcineurin nor blockage of P-glycoprotein play a role in the comparable induction of ROS production by CsA and its analogs.We found that CsA induces...

Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx

... attack of the L-hOrn3a-amino group onto l-haOrn4. Takinginto account that the synthetases involved in the bio-synthesis of the DKP-inheriting toxins thaxtomin and fumitremorgin also contain ... pathogens, the interest in the structural and functional characterization of these compounds isgrowing and may result in the synthesis of speciﬁcinhibitors based on the structure of the pathogensiderophore ... protein (etcE) and proteins for siderophore export and uptake (etcCFGK).A bioinformatic overview of the encoded proteins and the corresponding functions is provided in Table S1. The amino acid...

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... by the second gene in the las operon (Fig. 1). In order to obtain strains with increased PK activity anadditional copy of the pyk gene was recombined into the TP901-1 attachment site using the ... to result in equalamounts of formate and acetyl-CoA and the resultingacetyl-CoA is then metabolized into equal amount of ethanol and acetate to maintain the redox balance.Discussion In this ... for example, in order to save energy and protein synthesizing capa-city. This would be preferable to placing all the genesinvolved in the pathway in the same operon; the cellcan then respond...

Tài liệu Báo cáo khoa học: "Learning to Find Translations and Transliterations on the Web" doc

... 3.1.4 Generating distance feature. In the final stage of preparing training data, we add the distance, i.e. number of words, between a Chinese token feature and the English term in question, ... training and testing. We obtained a total of 155,310 article pairs, from which we then randomly selected 13,150 and 2,181 titles as seeds to obtain the training and test data. Since we are using ... submitting the given phrase as query to a search engine to retrieve snippets, and generate features for each tokens in the same way as done in the training phase. We then use the trained model...

Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt

... preparation. Of particular importance is the ﬁnding that exogenous cADPR failed to increase the spontaneous release of ATP in the absence of CD38. In other words, the presence of CD38 is mandatoryfor the ... suggesting that the two adenine nucleotides may work in synergy to maintain the restingtone of the bladder. In conclusion, extracellular cADPR enhances the spontaneous release of ATP in the bladder ... facilitatingeffects of cADPR. In contrast, the inhibitors of intra-cellular cADPR receptors 8-Br-cADPR (80 lm) and ryanodine (50 lm for 45 min) abolished the enhancingeffect of cADPR (Fig. 6). Therefore,...

Báo cáo khoa học: Mycobacterium tuberculosis possesses a functional enzyme for the synthesis of vitamin C, L-gulono-1,4-lactone dehydrogenase doc

... biosynthesis of colla-gen and other hydroxyproline ⁄ hydroxylysine-containing proteins ⁄ peptides; synthesis of secondary metabolites,hormones and cytokines [1]; oxidative protein folding and endoplasmic ... Escherichia coli. The recombinantprotein contained an engineered enterokinase cleavagesite in the junction between the GST tag and the Rv1771 sequence. Upon 3 h of induction of the pDEST15_Rv1771 ... protein band, thus pointing to the absence of a covalently bound ﬂavin moiety in the recombinant product. Moreover, as in the case of the plant l-galactono-1,4-lactone dehydrogenase [12],the...

Báo cáo khoa học: Novel c-carboxyglutamic acid-containing peptides from the venom of Conus textile docx

... 14 Da massincrease consistent with methylation of the side chaincarboxyl group of the N-terminal Asp residue conﬁrm-ing amidation of the C-terminus. Partial methylation of the internal peptide ... Gla(3)–TxVI:conﬁrmation of the presence of BrTrp. Product ionmass spectrum of the doubly charged ion at m ⁄ z ¼660.18. The isotopic distribution of the b2ion (inset)indicates the presence of bromine. The MS ... C-terminus.Cloning of cDNAs encoding the Gla(1)–TxVI,Gla(2)–TxVI/B and Gla(3)–TxVI precursors The isolated 580 bp cDNA encoding the Gla(1)–TxVIprecursor includes the 5¢- and 3¢-UTR and contains anORF of...

Báo cáo khoa học: Examining multiprotein signaling complexes from all angles The use of complementary techniques to characterize complex formation at the adapter protein, linker for activation of T cells pdf

... methods. These approachesoffer the opportunity to probe the basic mechanism of protein–protein interactions in great detail. First, the afﬁnity of the LAT-binding proteins, PLC-c1, Grb2 and Gads, ... for the lack of in vivo binding of Gads tophosphorylated LAT tyrosine 132) and the formation of multiprotein signaling complexes (the reason for the lack of detectable in vivo association of ... givendetailed information on the binding of speciﬁc SH2domain-containing signaling proteins to individualphosphorylated LAT tyrosines. They have also provi-ded substantial information on the composition...

Báo cáo khoa học: A Kazal prolyl endopeptidase inhibitor isolated from the skin of Phyllomedusa sauvagii pdf

... mMdithiothreitol, in a ﬁnal volume of % 350 lL, and by incubating the resulting solutions for 1 hat 37 °C in the dark. After reduction, 1 lLof4-vinylpyri-dine was added, and the incubation was continued ... bradykinins, caeruleins, bombesins, sauv-agine, opioid peptides (dermorphins and deltorphins),antimicrobial peptides (dermaseptins and adenoregulin), and tryptophylins [7,9–12]. Interest in these natural ... active against trypsin by replacingits P4,P5,andP6residues with the corresponding residues of acrosin inhibitor and proline at P1with lysine (Fig. 2). Thus,not only is the sequence of PSKP-1...

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