Báo cáo khoa học: Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis doc

... 2003 Tracking interactions that stabilize the dimer structure of starch phosphorylase from Corynebacterium callunae Roles of Arg234 and Arg242 revealed by sequence analysis and site-directed mutagenesis Richard ... structure. The results have revealed clearly that Arg234 and Arg242 of the TOWER interface region of StP partially destabilize the dimer structure of the unligated enzyme so that loss of theseresidues ... 773–783.12.Griessler,R.,D’Auria,S.,Tanfani,F.&Nidetzky,B.(2000)Thermal denaturation pathway of starch phosphorylase from Corynebacterium callunae: oxyanion binding provides the glue that eﬃciently stabilizes the dimer structure of the protein....

Báo cáo khoa học: "Tracking Initiative in Collaborative Dialogue Interactions" pptx

... represents the impact of the piece of evidence on the subsets of O. A bpa assigns a number in the range [0,1] to each subset of O such that the numbers sum to 1. The number assigned to the subset ... without cues. Furthermore, substantial improve- ment is gained by the use of counters since they prevent the effect of the "exceptions of the rules" from accu- mulating and resulting ... that while in the majority of turns, the task and dialogue initiatives are held by the same agent, in approximately 1/4 of the turns, the agents' behavior can be better accounted forby...

Tài liệu Báo cáo khoa học: Paradoxical interactions between modiﬁers and elastase-2 Patricia Schenker and Antonio Baici docx

... in the Ki by DS, and no effect on Ki by Ch6S (Table 2). The various effects of sulfated polysaccharides on inhibi-tion of elastase-2 by eglin c and by the tetrapeptidederived from his sequence ... in the absence orpresence of effectors, not to determine absolute values from their dependence on the concentration of eglin c and a1-PI. The effects of inhibition by a1-PI and eglin c by ... with the modiﬁers separately and then in various combinations of concentrations. In the case of the sulfated polysaccharides, the effectormolecules are constituents of the same sample, and their...

Báo cáo khoa học: Two CYP17 genes in the South African Angora goat (Capra hircus) – the identiﬁcation of three genotypes that differ in copy number and steroidogenic output pptx

... the steroidoutput of the adrenal. The discovery that the twoCYP17 isoforms are two genes and that the genotypesdiffer not only by the genes present, but also by the copy number, suggests that ... to identify the distribution of the two CYP17 allelesin the South African Angora population. These data revealed that the twoCYP17 isoforms were not the product of two alleles of the same gene, ... the target and reference genes. The PCR efﬁciencies were calculated from the slopes of the standard curves generated by light-cyclerÒ software (version 3.5) over two orders of magni-tude, and...

Báo cáo khoa học: Differential interactions of decorin and decorin mutants with type I and type VI collagens pptx

... indicate that the banded ﬁbrilforming collagens are traversed speciﬁcally near their ÔdÕbands, within the gap region of the collagen ﬁbrils, by the ﬁlamentous beaded structures of the type ... without the addition of complexes were performed. For the analysis of interactions between proteoglycans and collagens, the sensograms were corrected by a modiﬁcation of the method of Roden and Myszka ... composition of the collagen used in the experi-ments. Quantitative analysis of the SDS gel electrophoresesindicated that 63% of the type VI collagen contained the long chain and 37% the s hort...

Báo cáo khoa học: Hepatocyte growth factor activator (HGFA): molecular structure and interactions with HGFA inhibitor-1 (HAI-1) doc

... [4]. Other key attributes of the catalytically competent structure are the ‘oxyanionhole’ formed by the amide nitrogens of Ser195 and Gly193, and substrate-binding subsites (S1, S2, S3, and S4), ... chains of the catalytic triad residues (Asp102, His57, and Ser195) are indicated (yellowfor ‘open’; magenta for ‘closed’), as is that of the 220-loop residues 215 and 216. The P1 Arg from the KQLR-cmk ... widespread and forms part of the biological regulation of enzymeactivity. Examples of regulation also include Na+effects on thrombin [10], the PDZ domain of DegS[13], and interactions between the...

Báo cáo khoa học: Netropsin interactions in the minor groove of d(GGCCAATTGG) studied by a combination of resolution enhancement and ab initio calculations pot

... calculations The netropsin amidinium end The contact area of the Nt amidinium end (NAE) wasinvestigated by evaluating the interaction energies and hydrogen positions of the end fragment and the bases of ... of van der Waals’ interactions between the drug and the ﬂoor and walls of the minor groove [21,22]. Electrostatic interactions between the negatively charged minor groove and the positively charged ... the c-angle of G1 and C3 (trans; )171.3° and )173.3°, respectively), which is a consequence of triplex formation and the c-angle of G21 [(+)-ac,98.2°] due to the end-standing O5¢. The nucleosides...

Báo cáo khoa học: Metabolic pathway that produces essential fatty acids from polymethylene-interrupted polyunsaturated fatty acids in animal cells pot

... by cleavage between the methylthio-substituted carbons.They clearly showed that the two species of monoeno-ates formed by the partial hydrogenation of the unknown fatty acid were 16:1 D-7 and ... acid methyl esters pre-pared from the triacylglycerol fraction of the cells were analyzed by GC. The number at the top of the peak on the chromatogram cor-responds to the peak number in Table 1. ... 5D). Theseresults clearly show that peroxisomes are required for the formation of 16:2 D-7,10 from sciadonic acid, and that 16:2 D-7,10 is essential for the biosynthesis of linoleic acid. The...

Báo cáo khoa học: PSI1 is responsible for the stearic acid enrichment that is characteristic of phosphatidylinositol in yeast pdf

... stopped by the addition of 2 mL of chloroform ⁄ meth-anol (2 : 1, v ⁄ v) and 500 lL of water. The formed productswere extracted and analyzed as described above.Statistical analysis The level of ... in the de novo synthesis of a given phospholipid, includingPI. Hence, the rate of synthesis and the amount of CDP-DAG molecules that were used as substrate forPI synthesis appeared to be the ... directacylation of stearic acid at the sn-1 position of lysoPI and excluding the possibility of a transacylation mech-anism from the sn-2 to the sn-1 position of PI. We fur-ther carried out...

Báo cáo khoa học: Protein interactions in the sumoylation cascade – lessons from X-ray structures pdf

... likely that ubc9 and the UFD of uba2 interact at the a1 region. (b) The region below the a1 helix consists mainly of the N-terminal exten-sion of a1 and loops between the b2 and b3 strands and the ... E1 is composed of APPBP1 (APPbinding protein 1) and uba3. The domain structures of Aos1 and APPBP1 resemble the N-termini, and those of uba2 and uba3 resemble the C-termini, of the ubiqu-itin ... only the human SAP domain has been analyzed at the structural level. It is formed by a four-helical bundle and is thought to bind DNA [40]. The structures of the SP-RING domain and of the CTDs, the...

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