Báo cáo khoa học: A dimer of the FeS cluster biosynthesis protein IscA from cyanobacteria binds a [2Fe2S] cluster between two protomers and transfers it to [2Fe2S] and [4Fe4S] apo proteins ppt

... PCC6803byPCRusingtheprimers PRiscA11 (5¢-GGAATTCCATATGAGCCAAGCCACC GCTACC-3¢)andPRiscA12(5¢-GATCTAAGCTTAAA CCCCAAAGGATTTACC-3¢). The resulting 376-bp frag- ment was cleaved with NdeIandHindIII and cloned into the ... spectra, indicating that the FeS cluster was in a diamagnetic state. Addition of dithionite to the protein solution did not lead to the appearance of...

Ngày tải lên: 31/03/2014, 01:20

... in high-molecular-weight GTPases such as dynamin and the antiviral protein, Mx. Because of similarities in the molecular architecture of GBPs and members of the dynamin family, GBPs are classified as a part ... enterica, and a positive regulation of pyroptosis to defend against infection by S. enterica and possibly other bacterial pathogens [15]. Although no other...

Ngày tải lên: 15/03/2014, 10:20

... s at 55 °Cand30sat72°C, using two primers 5¢-GGAATTCCATATGGGAGTCAAGACTGA GAA CAAC-3¢ and 5¢-CCGCTCGAGTCAACCTCCCGTCT G-3¢. The DNA products were checked on 1.5% agarose gels stained with e thidium ... other hand, the yeast SMT3 (SUMO) protein is 40–45% identical to human SUMO proteins in amino acid sequence a nd the human SUMO proteins have an insertion between the stra...

Ngày tải lên: 16/03/2014, 18:20

... and harvested af ter 3 days. Part of the cells was usedastotalcelllysate(T),whilethe other part was fractionated into a soluble fraction (S) and a nuclear fraction (N). Fractions were analyzed by ... isothiocy- anate (FITC)-conjugated swine anti-rabbit IgG, FITC- conjugated rabbit a nti-human IgG, FITC-conjugated r abbit anti-mouse IgG, and tetramethylrhodamine isothiocyanate...

Ngày tải lên: 23/03/2014, 13:20

... Integra 700 autoanalyzer (Roche, Swit- zerland). Enzyme activity in the medium was calculated as percentage of the total intracellular and extracellular LDH activity. Toxicity of the iron preparations ... spectrophotometry (AAS) (Hitachi). The iron content of ferritin was calculated from the iron concentra- tion in the supernatant and the amount of ferritin within...

Ngày tải lên: 31/03/2014, 07:20

... GTA GGT TGA TTT CAT GTC GAA TG-3¢; additional XbaI site underlined) and OB 3 (5¢-AAA AGA ATT CTT AGA AGT CCC AGT CAT CGT C-3¢; additional EcoRI site underlined). The amplified PCR fragment (Taq ... of the tyrosyl- and manganese-containing small subunit. Here, the reaction was started by addition of CDP. Data are given as the mean of triplicates. Inhibitory effects of the...

Ngày tải lên: 15/02/2014, 01:20

... Based on surface analysis, we propose a rigid domain association for the N-terminal SEA domain with the back site of the proteinase domain. Abbreviations HAI, hepatocyte growth factor activator ... extracellular matrix, it seems more likely that the SEA domain functions by orienting the active site cleft of DESC1 toward plasma and ⁄ or extracellular spaces and away from...

Ngày tải lên: 19/02/2014, 00:20

... suggestive of a catalytic water molecule which has been replaced by the acetate moiety. The protein coordinates zinc with the N d atom of His12, one of the O d atoms of Asp15 and the N e atom of His113. His113 ... the active site into a cavity with a depth of 12 A ˚ and its diameter varies from 8 A ˚ at the bottom to 12 A ˚ near the edge (Fig....

Ngày tải lên: 19/02/2014, 00:20

... siRNA ZF5 (5¢-GAGGAAGCAUGA GAAACUCUU-3¢ and 5¢-GAGUUUCUCAUGCUUCCU CUU-3¢) matched bases 945–963; and the third siRNA ZF5 (5¢-GGUCCUGAACUACAUGUACUU-3¢ and 5¢-GUAC AUGUAGUUCAGGACCUU-3¢) matched bases ... Primer software developed by V. Prutkovsky and O. Sokur of the Institute of Influenza, Ministry of Health of the Russian Federation. The first pair of primers (5¢-GGCCTT...

Ngày tải lên: 07/03/2014, 05:20

... adult hypophosphatasia and (e) odonto hypophosphatasia [1–4]. Perinatal and infantile forms of hypophosphata- sia are severe and are usually transmitted as a recessive trait, whereas the other three forms of hypophosphata- sia ... transport and resultant degradation of TNSALP (V40 6A) in the endoplasmic reticulum, as the majority of newly synthesized TNSALP (V40 6A) wa...

Ngày tải lên: 07/03/2014, 05:20