... procedure. The finding that the effect of CoASH depends on the presence of ATP (althoughnot of other NTPs such as GTP and UTP; notshown) suggests that it is indirectly mediated via the formation of ... because of the presence of saccharo-1,4-lactone, an inhibitor of b-glucuronidase, in the sampleswhere b-glucuronides had to be determined, the amount of b-glucuronidase and the incubation time with ... 20%, despite the presence of ATP-Mg. It is shown below that this is due to loss of the inhibitory effect of ATP-Mg, consequent to the removal of a heat-stable cofactor present in the high-speed...