Báo cáo khoa học: Abortive translation caused by peptidyl-tRNA drop-off at NGG codons in the early coding region of mRNA potx

... prone to a codon dependent peptidyl-tRNA drop-off at the very begin- ning of the coding region in mRNA. In the case of NGG codons located close to the initiation codon such instability appears to ... associated with NGG in early coding reading in mRNA is the result of peptidyl-tRNA drop-off from the ribosome during translation in E. col...

Ngày tải lên: 30/03/2014, 20:20

... the apparent dissociation constant by Eqn (1). By assuming that the sample is saturated with Tb 3+ at the starting point of the competition, the Ca 2+ -binding affinity is further obtained using ... facilitate oligomerization, it is challenging to differ- entiate which region contributes to the oligomerization process. To overcome the limitations of investigating the...

Ngày tải lên: 07/03/2014, 00:20

... also increased (Fig. 1G,H). Taken together, these data AcLDL 1 2 Visfatin/cyclophilin mRNAVisfatin/cyclophilin mRNA Visfatin/cyclophilin mRNA CD36/cyclophilin mRNA FABP4/cyclophilin mRNA Visfatin/cyclophilin ... dampened in the presence of visfatin small interfering RNA or by the visfatin-specific inhibitor FK866. Taken together, our results suggest that PPARc regulates the...

Ngày tải lên: 06/03/2014, 22:21

... 2008). A rule has the form category := pattern and creates new annotation of the type category on any part of a text matching pattern. A rule for the annotation of passive clauses in the UAM CorpusTool ... annotation decisions that exceed a certain inter-rater agreement threshold. This annotation -by- query approach of querying, assessing, evaluating and annotating allows mu...

Ngày tải lên: 16/03/2014, 20:20

... precision using linguistic patterns, whereas surface patterns from Web frequency information contribute greatly to the coverage of relation extraction. • The combination of these patterns produces a ... clustering for relation cluster- ing. The dependency pattern has the properties of being more accurate, but the Web context has the advantage of containing much more redund...

Ngày tải lên: 23/03/2014, 16:21

... determined by following separately the strong signals of the aromatic residues of equine lysozyme and the oleic acid protons at 1.15 p.p.m. The diffu- sion coefficients determined by following the ... and the aromatic residue resonances of equine lysozyme in the region of 6.5–7.5 p.p.m., as shown in Fig. 6B. This indicates intermolecular binding between lysozy...

Ngày tải lên: 30/03/2014, 01:20

... the amino terminus of the pro- tein. Using this method, we showed that asparagine 26 represents the amino terminus of P40. Knowing, that the first amino acids of the processed P40 and P90 is the ... at its N-terminus instead of a free amino group [32]. Therefore, the asparagine in position 26 was labeled within the intact protein at its a-amino group and represent...

Ngày tải lên: 19/02/2014, 18:20

... string of terms and a structural representation is defined as a mapping between elements of the set of substrings of the string and elements of the set of subtrees of the tree : such a relation ... also note that the nodes in the TREE are simply concepts in the structural representation and thus the interpretation is independent of any grammar that defi...

Ngày tải lên: 22/02/2014, 10:20

... cleavage of dinucleoside polyphosphate molecules modified in their ADP moieties. In addition, none of the oxymethylene analogs investigated inhibited the hydrolysis of Ap 4 A catalyzed by the E. ... ab,a¢b¢-disubstituted analogs of Ap 4 A (e.g. ApCHFppCHFpA was cleaved at 3% of the rate of AppppA) [22]. They constitute a symmet- rical mode of cleavage of 5 by...

Ngày tải lên: 16/03/2014, 04:20

... of the side chain d-guanidino group of this residue are involved in H-bonding as follows: Ne with the O atom of the main chain CO group of the TrpB240; Ng 1 shares H bonding with O atom of the ... substrates docked in the active site of penicillin acylase by AM1 calculations. Amino acid residues of the enzyme are at their X-ray coordinates [19]. Substrates...

Ngày tải lên: 16/03/2014, 16:20