Báo cáo khoa học: Protein glycation in Saccharomyces cerevisiae Argpyrimidine formation and methylglyoxal catabolism ppt

... We investigated methylglyoxal metabolism and protein glycation in Saccharo- myces cerevisiae. Using a specific antibody against argpyrimidine, a marker of protein glycation by methylglyoxal, we ... cells [21]. Protein glycation by methylglyoxal in yeast, monitored by argpyrimidine formation in proteins, was evaluated in a set of null mutant yeast strains for ge...

Ngày tải lên: 30/03/2014, 20:20

... CaM-binding domains, and contains at least one conserved potential CaM-binding motif [46]. Additionally, the binding of CaM at the C-terminus and the binding of NADPH at the extreme N-terminus ... domains are degenerate 34-amino acid repeats forming anti-parallel a-helices known to be involved in an array of protein protein interactions (generally with non-TPR proteins) [41]. Both dom...

Ngày tải lên: 07/03/2014, 03:20

... short- period-sustained oscillation of Saccharomyces cerevisiae. Yeast 12, 673–682. 8 Satroutdinov AD, Kuriyama H & Kobayashi H (1992) Oscillatory metabolism of Saccharomyces cerevisiae in continuous ... tre- halose in whole yeast cells. Anal Biochem 248, 186–188. 34 Hans MA, Heinzle E & Wittmann C (2003) Free intra- cellular amino acid pools during autonomous oscilla- tio...

Ngày tải lên: 30/03/2014, 16:20

... their role in precursor protein recognition and receptor protein function. Toc159 was first identified by chemical cross-linking at both the reversible and stable binding stages of prepro- tein import ... preproteins. AtToc12 (12) exposes a J-domain (J) into the intermembrane space and has a role in anchoring Hsp70, thereby assisting in the transfer of prepro- teins to the trans...

Ngày tải lên: 07/03/2014, 03:20

... for proteinaceous insertion machinery. Other single- spanning proteins have also been suggested to use this membrane integration route, including the photosys- tem II subunits, PsbW and PsbX. In ... undergoes conformational rearrange- ment during active protein transport, with the amphi- pathic helix and C-terminal tail interacting only in response to conditions leading to protein...

Ngày tải lên: 07/03/2014, 03:20

... mitochondrial and chloroplastic targeting signals in terms of arginine and serine con- tent (i.e. not as high as in mitochondrial targeting signals) and may be slightly enriched in hydrophobic residues. In ... raises interesting questions for inter-organelle communication. A greater understanding of the process of dual targeting may provide useful insights into the targeting of...

Ngày tải lên: 07/03/2014, 03:20

... ª 2007 FEBS Nucleosome positioning in relation to nucleosome spacing and DNA sequence-specific binding of a protein Rama-Haritha Pusarla*, Vinesh Vinayachandran* and Purnima Bhargava Centre for ... the nucleosome-spacing effects on the bulk chromatin. The distance between protein- binding sites decides nucleosome positioning Positioning of five nucleosomes in a single register as a r...

Ngày tải lên: 16/03/2014, 10:20

... strain in glucose med- ium (Figs 10A and S4), indicating that, although these proteins show sequence similarity in the C-terminal portion, divergences in the remaining sequence of the protein ... two-hybrid assays Fusion proteins with either lexA DNA-binding domain (BD -protein) or Gal4p transcription activation domain (AD -protein) were expressed in the host strain L40 [39], whic...

Ngày tải lên: 22/03/2014, 21:21

... were washed off and bound preproteins eluted with 250 mm imidazole containing buffer. In every case, precursor protein was detected in the imid- azole eluate, indicating a direct interaction with Toc34DTM. ... Toc75 preprotein contains a bipar- tite targeting signal. The N-terminal domain is respon- sible for chloroplast targeting and translocation initiation. Translocation is halte...

Ngày tải lên: 23/03/2014, 07:20

... reaction. Abbreviations APPBP1, APP binding protein 1; CtBP, C-terminus binding protein; CTD, C-terminal domain; E1, activating enzyme; E2, conjugating enzyme; E3, ligase; HECT, homologous to E6AP C-terminus; IR, internal ... in Nedd8, and Arg72 in ubiquitin (highlighted in yellow in Fig. 2C). Matching E1 residues are Thr149 in uba2, Arg190 in uba3 and Gln608 in uba1 (hig...

Ngày tải lên: 30/03/2014, 04:20